Industrial production method of corn protein polypeptide from corn protein powder by enzymatical process
A corn gluten meal, corn gluten technology, applied in fermentation and other directions, can solve the problems of difficult control of the reaction process and the final product, amino acid damage, difficult to filter and other problems, and achieve the effect of improving scientific and technological content and quality, small molecular weight, and easy filtration.
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[0023] Accompanying drawing is a kind of specific embodiment of the present invention. As shown in Figure 1, this embodiment includes the following steps:
[0024] 1. Raw material requirements
[0025] ③ Corn Gluten Powder: Moisture ≤6.0%, Protein ≥64%, Ash ≤2.0%
[0026] ④Ion-free water: PH7.5-10 conductivity≤50us / cm
[0027] ⑤Enzyme preparation: α high temperature amylase 20000u / g alkaline protease 360000u / g
[0028] Neutral protease 130000u / g
[0029] 2. Put in 1kg of corn gluten powder, add 11500-7500ml of deionized water (sizing concentration 8-12%) after feeding, heat at 95-98°C and soak for 1 hour, and cut through the emulsifying shear at the bottom of the tank. and flow back into the tank.
[0030] 3. Add 15-20ml of 30% calcium hydroxide to adjust the pH of the feed solution to 6.5-7.0 and heat to 95-98°C, add 6.25-8.0ml of 0.625-0.8% α high-temperature amylase, and start enzymatic hydrolysis. 0.5 hours pH6.0-6.4 plus 30% calcium hydroxide 8-15ml pH6.5-7.0, 1.5 h...
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