708 results about "Complex protein" patented technology

The invention discloses small molecule polypeptide Ca-chelate of fishbone, which belongs to the field of functional food or additive. The method for preparing the small molecule polypeptide Ca-chelate of the fishbone comprises the following steps: boiling the fishbone at high temperature and high pressure; pulping to obtain the fishbone paste; utilizing the compound protease to execute enzymolysis; centrifuging to obtain the liquid supernatant and the sediment; filtering the liquid supernatant to obtain the small molecule polypeptide liquid of the fishbone; executing the composite acid acidification through citric acid and lactic acid for the sediment so as to obtain the fishbone calcium liquid; mixing the small molecule polypeptide liquid of the fishbone with the fishbone calcium liquid; executing the chelation at 40-50 degrees centigrade while the pH value is 7.5-8.5; and drying to obtain the small molecule polypeptide Ca-chelate of the fishbone. The invention has high Ca-chelate rate which reaches over 90%, and has high biological absorption rate of the calcium which reaches over 70%. The invention has no side effects, has good flavor, can be directly absorbed from intestinal mucosa after being eaten, solves the problems that the solubility of the traditional calcium source is bad, the absorption rate is low and the side effect is large, and solves the problem of producing calculi. In addition, the invention has a simple preparation method and low cost.

The invention provides a theoretical design and technical design of cell-free protein synthesis for DNA replication, transcription and translation coupling, a preparation, a kit and a preparation method. Specifically, with the application of the in-vitro cell-free synthesis system provided by the invention, complex protein can be synthesized, and moreover, DNA and mRNA can be synthesized; and effective, high-throughput and quite convenient protein synthesis can be completed with the use of a DNA template by a minute quantity (nanogram-microgram).

The invention discloses a method and process for extracting animal micro-molecule polypeptides and amino acids by utilizing a complex enzyme. The method comprises the following steps: (1) crushing animal tissues so that the animal tissues can pass through a sieve of 80-200 meshes, and adding water to prepare a suspension with the concentration of 8-30 percent; (2) raising the temperature to be 35-40 DEG C, regulating the pH value to be 8.0-9.0 by using sodium carbonate, adding a complex enzyme I accounting for 0.5-2.0 percent of the animal tissues, performing ultrasonic enzymolysis at 14-16 kHz for 1-2 hours so that the proteins between cells are subjected to full enzymolysis, regulating the pH value to be 6.5-7.5, adding a complex enzyme II accounting for 0.5-3.0 percent of the animal tissues, fully and uniformly stirring, controlling the temperature of the water bath to be 37-45 DEG C, and performing ultrasonic enzymolysis at 15-17 kHz for 1-6 hours; (3) raising the temperature of the enzymolysis solution to be 90-100 DEG C to inactivate the enzyme for 20-30 minutes after the enzymolysis is finished; (4) filtering the solution subjected to enzymolysis through a 0.22mu m microfiltration membrane; and (5) concentrating the filtrate to the relative density of 1:1.0-1:1.2 to prepare the concentrated solution, wherein the complex enzyme II consists of Alcalase, Flavourzyme, Protamex, neutral protease and papain. According to the method, the enzymolysis efficiency can be improved, and the production cost can be reduced.

A system and method for performing rapid, automated and high peak capacity separations of complex protein mixtures through the combination of fluidic and electrical elements on an integrated circuit, utilizing planar thin-film micromachining for both fluidic and electrical components.

The invention provides a protein synthesis system for in-vitro protein synthesis, a kit and a preparation method for a protein through in-vitro synthesis. Specifically, the in-vitro cell-free expression system provided by the invention is capable of extremely efficiently synthesizing proteins and synthesizing complex proteins. Moreover, due to the in-vitro cell-free expression system disclosed bythe invention, the relative light unit value of the activity of the synthesized luciferase is higher than that of the conventional commercial system (such as a rabbit reticulocyte in-vitro expressionsystem) by at least one order of magnitude (more than or equal to 10 times or higher).

The invention discloses fishbone bioactive polypeptide calcium powder and a preparation method, belonging to the field of functional food or food additives. The preparation method comprises the following steps: freezing fishbone, coarse grinding, fine grinding, adding water, stirring, adding a compound protease to hydrolyze, centrifugating, and respectively collecting the supernatant and the precipitate; ultrafiltering the supernatant by a filter membrane, concentrating the filtrate, and freeze-drying to obtain the fishbone protein polypeptide powder; washing the precipitate, drying and pulverizing; activating by using citric acid, and drying to obtain the soluble calcium; and mixing the fishbone protein polypeptide powder and the soluble calcium to obtain the fishbone bioactive polypeptide calcium powder. The fishbone bioactive polypeptide calcium powder enables the biological absorptivity of calcium to reach above 65%, and meanwhile, maintains the functional characteristics of polypeptide. Hydrolysis is carried out by using the food-grade protease, and thus, the fishbone bioactive polypeptide calcium powder has the advantages of high safety and no side effect. The method of the invention has the advantages of simple and feasible realization, and low cost. The fishbone bioactive polypeptide calcium powder can be used as a food additive or directly eaten as nutritional food.

The invention provides egg white powder with high foamability and a preparation method thereof and relates to reconstruction of whey protein structure and functional properties, belonging to the technical field of biological processing of foodstuffs. According to the invention, on the basis of preliminary work, enzymatic hydrolysis of lipase in advance and cooperative enzymatic hydrolysis of composite protease are utilized for treatment of egg white; total usage amount of lipase and composite protease is less than usage amount of individually used lipase or protease, and however, foamability and foam stability of egg white powder obtained by combined utilization of lipase and composite protease are higher than those of egg white powder obtained by individual utilization of lipase or composite protease; the egg white powder obtained in the invention can meet demands for high-grade products on the market, and the advantages of a simple process and high cost performance are achieved in the invention. According to the invention, the ratio of active usage amount of Aspergillus oryzae protease, papain and trypsin is determined to be 1:1:1; the usage amount and other technological parameters cooperatively allow egg white powder with high foamability to be obtained; egg white powder with high foamability provided in the invention enables the additional output value of eggs to be increased, lays a technical foundation for development and industrial production of special-purpose egg white powder products and increases economic benefits for enterprises.

The invention relates production method of soybean peptide, using the degreasing soya bean waste/ soybean powder as raw material, comprising the following steps: 1 solid fermenting: mixing the water with raw material in proportion by weight, inoculating zymocyte; 2 liquid enzymolysis: mixing the solid culture with water in proportion by weight, adding composite proteinase, carrying out enzymolysis; 3 extinguishing bacterium: catabolite extinguishing bacterium; 4 filter liquor: separating soybean peptide solution from enzymolysis liquid; 5 decolourization; 6 refining; 7 sterilization, and getting the product. The advantages are following: absorbing the advantages of liquid invoice method and liquid enzymolysis method, overcoming the defects of them, reducing the cost, decreasing the content of added enzyme, getting the high hydrolysis rate, and extending the soybean peptide application.

The invention discloses a unique extracting method of maize albumen powder from maize, which comprises the following steps: using composite proteinase to enzymolyze zein; separating; hyperfiltering; condensing; spraying; drying; obtaining the white powder shaped product. The invention simplifies the technique and shortens the manufacturing period with good taste and small molecular weight of hybrid peptide, which can be clinical nutrition, hygienic food, sports food and cosmetics.

The invention belongs to the field of food processing, and relates to a method for hydrolyzing egg-white proteins by various proteases. The method comprises the following steps of: first, heating obtained egg white to degenerate; and then, hydrolyzing egg-white proteins simultaneously or in stages by using two or more of compound protease, neutral protease, alkaline protease and flavourzyme. According to the invention, different enzymes are combined in use and compensate one another in enzyme acting sites, so that the degree of hydrolysis is improved, and the yield of egg-white protein peptide is increased.

The invention discloses a processing method of sea cucumber polypeptide, which is characterized by comprising the following steps: A. pretreatment: cleaning fresh sea cucumber, pulping and homogenizing the sea cucumber to obtain sea cucumber pulp; B. enzymolysis: adding papain and compound protease without adjusting the pH value to form a multienzyme system with the autolytic enzyme contained in the fresh sea cucumber so as to perform enzymolysis, and adding flavourzyme to continue enzymolysis until enzymolysis is finished; C. classification: commonly filtering and micro-filtering, and classifying the sea cucumber polypeptide enzymatic hydrolysate through ultrafiltration classification and gradient dilution; and D. package: drying and packaging the sea cucumber polypeptide in a moisture proof manner. According to the invention, the papain and the compound protease have a synergistic effect in the enzymolysis system to reinforce the enzymolysis effect, the whole enzymolysis process is convenient to operate and is beneficial for the mass scale production demand, and meanwhile, the separation efficiency is improved, classified sea cucumber polypeptide with high purity and quality is obtained, and no organic solvent is added in the sea cucumber polypeptide, so that the safety is high, and a guarantee is provided for developing the sea cucumber polypeptide.

The invention relates to a technology for synchronously preparing walnut oil and walnut peptide by means of microwave-assisted enzymolysis, belonging to the technical field of the food or the health food. The technology comprises the following steps of: removing the coat and the core of the walnut, grinding the walnut into walnut slurry, adding the water to prepare the slurry, hydrolyzing by adding the protease or the composite protease, treating by microwave, and centrifugally separating the oil phase, the peptide water phase and the residual solid phase, wherein the obtained oil phase is the cleaning and bright walnut oil which can meet the requirement of the green food; and further physically refining the oil phase at low temperature to be taken as the raw material oil of the high-class edible oil, the health food oil, the blend oil and the like. The obtained walnut peptide water solution can be directly used for preparing the degreased walnut peptide beverage by means of the enzyme inactivating, or can be used for preparing the active polypeptide product such as the walnut antioxidant peptide by means of the ultra-filtrating for the health food, the food additive, the nutrition reinforcing agent, the cosmetic, the daily chemicals and the like, or can be used for preparing the walnut peptide powder by means of the mist spray drying at low temperature. The technology improves the extraction efficiency of the walnut oil and the walnut peptide, and is simple in equipment, short in period, safe, low in energy consumption, high in use ratio and free of three-waste pollution.

Methods and compositions are described for analyzing complex protein mixtures, such as proteomes, using activity-based probes. In particular, probes that specifically react with and bind to the active form of one or more target proteins are employed. Labeled peptides obtained from the labeled active target proteins can be used in screening and identification procedures, and can be related to the identity, presence, amount, or activity of active members of the desired target protein class. The methods and compositions described herein can be used, for example, to provide diagnostic information concerning pathogenic states, in identifying proteins that may act as therapeutic targets, and in drug discovery.

Provided are protein microarrays, their manufacture, use, and application. Protein microarrays in accordance with the present invention are useful in a variety preoteomic analyses. Various protein arrays in accordance with the present invention may immobilize large arrays of proteins that may be useful for studying protein-protein interactions to improve understanding of disease processes, facilitating drug discovery, or for identifying potential antigens for vaccine development. The protein array elements of the invention are native or modified proteins (e.g., antibodies or fusion proteins). The protein array elements may be attached directly to a organic functionalized mirrored substrate by a binding reaction between functional groups on the substrate (e.g., amine) and protein (e.g., activated carboxylic acid). Techniques for chemical blocking of the arrays are also provided. The invention contemplates spotting of array elements onto solid planar substrates, labeling of complex protein mixtures, and the analysis of protein binding to the array. The invention also enables the enrichment or purification, and subsequent sequencing or structural analysis of proteins that are identified as differential by the array screen. Kits including protein-binding microarrays for proteomic analysis in accordance with the present invention are also provided.