Method and process for extracting animal micro-molecule polypeptides and amino acids by utilizing complex enzyme

Abstract

The invention discloses a method and process for extracting animal micro-molecule polypeptides and amino acids by utilizing a complex enzyme. The method comprises the following steps: (1) crushing animal tissues so that the animal tissues can pass through a sieve of 80-200 meshes, and adding water to prepare a suspension with the concentration of 8-30 percent; (2) raising the temperature to be 35-40 DEG C, regulating the pH value to be 8.0-9.0 by using sodium carbonate, adding a complex enzyme I accounting for 0.5-2.0 percent of the animal tissues, performing ultrasonic enzymolysis at 14-16 kHz for 1-2 hours so that the proteins between cells are subjected to full enzymolysis, regulating the pH value to be 6.5-7.5, adding a complex enzyme II accounting for 0.5-3.0 percent of the animal tissues, fully and uniformly stirring, controlling the temperature of the water bath to be 37-45 DEG C, and performing ultrasonic enzymolysis at 15-17 kHz for 1-6 hours; (3) raising the temperature of the enzymolysis solution to be 90-100 DEG C to inactivate the enzyme for 20-30 minutes after the enzymolysis is finished; (4) filtering the solution subjected to enzymolysis through a 0.22mu m microfiltration membrane; and (5) concentrating the filtrate to the relative density of 1:1.0-1:1.2 to prepare the concentrated solution, wherein the complex enzyme II consists of Alcalase, Flavourzyme, Protamex, neutral protease and papain. According to the method, the enzymolysis efficiency can be improved, and the production cost can be reduced.

Description

technical field [0001] The invention belongs to the technical field of protein engineering, in particular to a method and process for extracting animal small molecule polypeptides and amino acids by using compound enzymes. Background technique [0002] Compared with plants, the composition and ratio of various proteins in animals are closer to those of humans, so the protein components in animals have higher nutritional value for humans, especially some small molecular polypeptides and peptides with specific physiological functions in animals. Amino acids (biologically active substances) play an important role in regulating human metabolism. Traditional animal products are mainly processed by physical methods (such as crushing, high temperature, etc.), which not only have low extraction efficiency, but also cause a large amount of active ingredients to be destroyed and lose their biological activity. Although bio-enzyme technology is used in individual processing processes,...

AI Technical Summary

Problems solved by technology

Traditional animal products are mainly processed by physical methods (such as crushing, high temperature, etc.), which not only have low extraction efficiency, but also cause a large amount of active ingredients to be destroyed and lose their biological activity.

Although bio-enzyme hydrolysis technology is used in individual processing processes, a problem has been seriously ignored (the selection of enzymes should match the structure of animal tissues), which makes the degradation effect of enzymes unsatisfactory.