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Method for preparing fishskin fish-scale collagen protein

A technology of fish scale collagen and collagen peptide, which is applied in the direction of animal/human protein, specific peptide, connective tissue peptide, etc., can solve the problems of process combination, achieve low cost, high yield, and reduce production cost.

Inactive Publication Date: 2015-11-11
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The utilization of collagen first started from terrestrial animals, but the collagen products of terrestrial animals are difficult to meet people's needs. Therefore, in recent years, people have turned their attention to aquatic animals with richer collagen resources, especially freshwater and marine fish
Qin Yuqing et al. studied the optimal process conditions for squid skin collagen extraction (Research on Traditional Chinese Medicine, 2002, 1: 20-21), and Wang Jingfeng et al. (Chinese Patent Application No. 200710013140.2) provided a preparation method of squid skin collagen active peptide , they all prepare collagen or collagen active peptide separately, and do not combine the two preparation processes to achieve more full utilization of raw materials

Method used

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  • Method for preparing fishskin fish-scale collagen protein

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Embodiment 1

[0026] In an embodiment of the present invention, a preparation method of fish skin and fish scale collagen peptides comprises the following steps:

[0027] Step 1: Treatment of fish skin

[0028] Use fish skin as raw material, soak it in NaOH solution containing 0.04mol / L 6 times the weight of the raw material at a soaking temperature of 25°C for 5 hours, remove impurities and fat, rinse with water until neutral, use 0.3 mol / L 12 times the weight of the raw material The mol / L HCl solution was soaked at a soaking temperature of 25°C for 2 hours, rinsed with water until the pH was 4, and then ground to prepare a collagen solution for fish skin.

[0029] Step 2: Treatment of fish scales

[0030] 1) Pretreatment: Put the fish scales into a water tank, add water 15 times the weight of the fish scales, and soak for 3 hours to rehydrate the fish scales.

[0031] 2) Decalcification: send the rehydrated fish scales into an acid-resistant water tank, add 1% hydrochloric acid 14 times...

Embodiment 2

[0042] In the embodiment of the present invention, in the embodiment of the present invention, a preparation method of fish skin and fish scale collagen peptide comprises the following steps:

[0043] Step 1: Treatment of fish skin

[0044] Use fish skin as the raw material, soak in 0.07mol / L NaOH solution 9 times the weight of the raw material at a soaking temperature of 35°C for 6 hours, remove impurities and fat, rinse with water until neutral, use 0.4 mol / L 15 times the weight of the raw material The mol / L HCl solution was soaked at a soaking temperature of 35°C for 4 hours, rinsed with water until the pH was 6, and ground to prepare a collagen solution for fish skin.

[0045] Step 2: Treatment of fish scales

[0046] 1) Pretreatment: Put the fish scales in a water tank, add water 18 times the weight of the fish scales, and soak for 4 hours to rehydrate the fish scales.

[0047]2) Decalcification: send the rehydrated fish scales into an acid-resistant water tank, add 2% ...

Embodiment 3

[0058] In the embodiment of the present invention, in the embodiment of the present invention, a preparation method of fish skin and fish scale collagen peptide comprises the following steps:

[0059] Step 1: Treatment of fish skin

[0060] Use fish skin as raw material, soak it in NaOH solution containing 0.05mol / L 7.5 times the weight of the raw material at a soaking temperature of 30°C for 5.5 hours, remove impurities and fat, rinse with water until neutral, use 13 times the weight of the raw material Soak in 0.35 mol / L HCl solution for 3 hours at a soaking temperature of 30°C, rinse with water until the pH is 5, and grind to prepare a collagen solution for fish skin.

[0061] Step 2: Treatment of fish scales

[0062] 1) Pretreatment: Put the fish scales into a water tank, add water 16.5 times the weight of the fish scales, and soak for 3.5 hours to rehydrate the fish scales.

[0063] 2) Decalcification: send the rehydrated fish scales into an acid-resistant water tank, a...

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Abstract

The invention discloses a method for preparing fishskin fish-scale collagen protein. The method includes the steps of processing fishskin, processing fish scale, preparing collagen protein and the like. The fish scale processing includes the steps of preprocessing, decalcifying, neutralizing and removing immunities, washing, injecting sol, solid and liquid separation, filtering and obtaining a water-soluble collagen solution generated after fish scale is removed. The collagen protein preparing includes the steps of hydrolysis, enzymolysis, collagen protein filtering and separation, enzyme deactivation, decolorization, fishy smell elimination, drying and the like. The ash content in obtained collagen peptide is obviously lower than that of other enzymolysis methods, and no fishy smell exists. According to the method, hydrolysis is conducted on collagen through compound protease and bromelain, the yield of micromolecule collagen peptide is high, and the enzymic preparation is low in cost and free of bitter taste. By means of the method, the production cost of the collagen peptide can be lowered remarkably, the obtained collagen peptide is white and tasteless powder, the ash content is smaller than 0.5%, and peptide fragment with the molecular weight smaller than 3000 Da accounts for over 96% of total nitrogen.

Description

technical field [0001] The invention relates to the field of fish collagen peptide preparation, in particular to a preparation method of fish skin and scale collagen peptide. Background technique [0002] Collagen is a high molecular functional protein. It is the main component of the skin, accounting for 80% of the skin's dermis. It forms a fine elastic network in the skin, which firmly locks in moisture and supports the skin. Collagen is a helical fibrous protein twisted by 3 peptide chains, and it is also the most abundant protein in the human body. It accounts for 30% or more of the total protein in the human body. It is rich in proline, hydroxyproline and other collagen characteristic amino acids needed by the human body. It is an important part of human cells, especially the extracellular matrix of the skin. Therefore, collagen is also the most popular beauty and health care product in the beauty industry in developed countries. . [0003] Active collagen has a va...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/34
Inventor 周春霞洪鹏志杨萍刘唤明
Owner GUANGDONG OCEAN UNIVERSITY
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