103 results about "Egg albumin" patented technology

This invention discloses one enzyme immune test method and agent case for 3- methyl quinoline 2-carboxyl acid in immune chemical analysis technique field, which comprises the following steps: processing immune antigen and cover antigen and alloantibody and pre-processing sample and establishing ELISA method; the matched agent case is composed of 3- methyl quinoline 2-carboxyl acid alloantibody covered by MQCA and egg albumin couple and MQCA standard sample; the sample is processed through metaphosphoric acid to release MQCA and MAX and indirectly competition ELISA method.

A conjugate of norfloxacin is prepared from norfloxacin semi-antigen and the bovine serum albumin (or egg albumin) as the carrier able to generate immunogenicity through coupling. It can be used for preparing the reagent kit for the enzyme-linked immunoassay of norfloxacin.

A nano colloidal golf linked immunoassary for detecting carbofuran includes such steps as coupling the immunized semi-antigen of carbofuran with BSA, synthesizing artificial immunoantigen compound, immunizing animal to prepare specific high-valence BFNH antibody, separating and purifying, linking the nano-class colloidal golf to said specific antibody, solidifying said linked compound, carbofurancoupled by egg albumin and sheep's mouse IgG antibody on a carrier, and for semi-quantitative analysis. Its advantage is high sensitivity and speed.

The invention discloses an enzyme-linked immunoassay kit for acid orange II, which belongs to the technical field of enzyme-linked immunosorbent analysis. Reagents in the kit provided by the invention comprise an enzyme label plate coated by a coupled substance of the acid orange II serving as a coating antigen and a carrier protein, standard solution of the acid orange II, solution of enzyme labeled goat-anti-rabbit antigen, antibody solution of the acid orange II, luminous solution, washing solution, coating solution and closed solution. The carrier protein of the coupled substance is bovine serum albumin or egg albumin with a molecular weight of 6.7 to 6.8 KDa. The coating antigen of the coated enzyme label plate is prepared by coupling the acid orange II and egg albumin, and an artificial immunogen for preparing the angiten is prepared by coupling the acid orange II and the bovine serum albumin. The maximum acid orange II detection range of the kit is 0.1 to 10 ng / mL. The enzyme-linked immunoassay kit of the invention has the characteristics of simplicity, quickness, accuracy and high sensitivity and can play an important role in the acid orange II detection of synthetic non-edible pigment in foods (such as marinated products and cayenne pepper) and drinks.

The invention belongs to the immunity analysi technical field, in particular to a reagent box for qualitatively detecting the content of ochratoxin A in food and the detecting method thereof. The reagent box comprises a test paper strip and reagent, wherein, the test paper strip is of a solid support which takes a joining enveloped with the ochratoxin A and egg-albumin as a detecting area A of the test pater, and takes a goat anti-rabbit secondary antibody as an effective standard area B of the test paper strip. The invention has the advantages of simple operation, high detecting sensitivity, strong specificity, good veracity, low detecting cost, better stability, etc., in addition, the range of application is wider. The invention is mainly used for the qualitative detection of the content of the ochratoxin A in the food and the provision.

The invention relates to the field of production of high-purity egg albumin polypeptide, and especially discloses a method for preparing egg albumin polypeptide from egg albumin powder by an enzymic method. According to the method for preparing egg albumin polypeptide from egg albumin powder by an enzymic method, the egg albumin powder is used as raw material; and the method provided by the invention is characterized by comprising the following steps of: dissolving the egg albumin powder in deionized water, adding a papain solution for hydrolysis, then, heating up the solution to be boiling, preserving heat and deactivating enzyme; executing membrane filtration to collect filtrate from the solution treated by enzyme deactivation; concentrating the filtrate in a vacuum low-temperature triple effect concentrating pan, and then, adding the active carbon, heating up, preserving heat and executing frame filtering, and carrying out spray drying for the filtrate to get the finished product. The method provided by the invention is simple in steps, low in production cost, exhaustive in enzymolysis, short in reaction time, easy to filter, high in peptide yield and capable of greatly improving the technological content and the quality of products, and being used and popularized widely.

The invention discloses an extracting and purifying method of salviol acid A, which comprises the following steps: adopting salvia miltiorrhizae medicine materical crude slice or powder as raw material; using solvent to extract; condensing to obtain the condensate of salvia miltiorrhizae; obtaining high-purity product through protein adsorption and solvent extracting method; using gelatin or egg albumin to sediment salviol acid A; dissolving the salviol acid A through acetone solution; analyzing; extracting through organic solvent to obtain the high-purity product with receiving rate over 0. 3% and purity over 90%. The invention has advantages of reasonable design, high extraction rate, good purity, stable property, simple technique and low manufacturing cost without column chromatography, which is fit for industrial manufacturing.

The invention discloses bovine lactoferricin-human lysozyme fused protein. The bovine lactoferricin-human lysozyme fused protein is characterized by comprising porcine myofibrillar protein antioxidative peptide, bovine lactoferricin, hen egg white protein antioxidative peptide, porcine muscle myosin antioxidative peptide, flexible peptide and human lysozyme which are connected in series. Through anionic antioxidative peptide derived from fusion expression animals, the positive charge of antimicrobial peptide is neutralized, and inhibition to host bacteria by antimicrobial peptide is reduced; antioxidative peptide is beneficial to the improvement of storage stability of the antimicrobial peptide, the antibacterial activity to escherichia coli (ATCC 25922) by the fused protein is only reduced by 7.7 percent after the fused protein is laid for 20d at 4 DEG C, and the loss of activity to the escherichia coli (ATCC 25922) by the fused protein is only reduced by 15.4 after the fused proteinis laid for 30d.

The invention provides a method for preparing egg albumin peptide with high antioxidant activity. The specific steps are as follows: modifying egg albumin suspension at 85-95 DEG C for 10 minutes, cooling to room temperature, respectively hydrolyzing the egg albumin suspension for 1-6 hours by using alkaline protease and pepsase, performing enzyme deactivation, cooling and centrifuging to extract supernatant; ultrafiltering the supernatant by using a membrane with molecular weight cut-off of 3kDa, and collecting filtrate; carrying out vacuum concentrating and frozen drying to obtain white or faint yellow egg albumin antioxidant peptide rough product; adsorbing the filtrate by using DA201-C macroreticular resin, carrying out gradient eluting by using ethanol, collecting, performing vacuum concentrating and frozen drying on a 75% ethanol elution component to obtain egg albumin peptide with high antioxidant activity. The method provided by the invention has the advantages of high egg albumin utilization rate, simple process, low cost and convenient industrial production; the obtained egg albumin peptide has significant antioxidant activity and is safe and free of toxic side effect.

A process for producing an arginine-rich peptide mixture and the application thereof in cervical cancer therapy is provided. The process includes the following steps: A suspension of walnut meal and egg albumin is pretreated with ultrahigh pressure, and then digested by alkaline proteinase and papain in separated steps with the ultrasonic and microwave-assisted extraction. The peptides of interest are isolated from filtration supernatant obtained after the enzyme digestion by reversed phase high-performance liquid chromatography. By using the peptide mixture as a template, acrylic acid and methyl acrylic acid as functional monomers, triethylene glycol dimethacrylate as cross-linking agent, and isopropylthioxanthone in acetone as a photoinitiator, polymerization is induced by ultraviolet light to form a surface imprinted membrane for isolating and enriching the peptides of interest from the supernatant. The arginine content in the peptide mixture is more than 18%. The arginine-rich peptide mixture is able to strongly suppress the proliferation of human cervical cancer Hela cells. The approach is applicable to reduce the cost of production and speed up the commercialization of large-scale production.

The invention discloses an immunoassay method for a nano-colloidal gold marker of tadalafil and analogs thereof. The determination method comprises the following steps: coupling oximate tadalafil with bovine serum albumin by adopting an activated ester method, synthesizing artificial immunity antigen which is used for immunizing animals, and preparing specific tadalafil and analogs thereof; in addition, coupling the oximate tadalafil with egg albumin to be used for construct an immunity detection method; separating, purifying and labeling the tadalafil and analogs thereof to the nano-colloidal gold, and then respectively curing the tadalafil and analogs detection antibodies thereof and goat-anti-rabbit IgG on a carrier, and adding a detection antigen into a detection solution. According to the method, the lowest detection limit of the tadalafil and analogs thereof legally added into health products and Chinese patent medicines is 10mu g / kg, and the fast detection can be completed within 5 minutes. The method is stable, fast and accurate, and suitable for one-step fast detection on legally added medicines.

The invention discloses an ofloxacin coupling compound with general formula (I), which comprises coupling compounds of ofloxacin hapten and bovine serum albumin of carrier substance which can produce immunogen or egg albumin. Thereinto, n stands for molecular number of ofloxacin combined with a bovine serum albumin molecule, the said n is an integer between one and twenty, and BSA stands for bovine serum albumin with 6.6KDa-6.9KDa of molecular weight. The invention also discloses a process for preparing the said coupling compound, which consists of joining ofloxacin and carrier substance which can produce immunogen to obtain the said coupling compound which induces immune system of animals to produce antibody. By immuning white rabbits from New Zealand, the ofloxacin coupling compound of this invention has prepared antiserum with 1:512,000 of potency, of which the lowest check limit is 0.1ppb. The invention is characterized in that it is simple, rapid, specific, exact and so on, which provides a foundation for preparing enzyme immunoassay agent box of ofloxacin.

The invention belongs to fields of immunochemistry analysis. It discloses an enzyme-uniting immune method and reagent box used in detection of quinoxaline-2-carboxyl acid residues, the method mainly contain medicine reconstruction, the preparation of immunogen, peridium antigen and antibody and pretreatment of sample and building of ELISA detecting method. The reagent box comprises quinoxaline-2-carboxyl acid (QCA) specificity antibody, QCA standard substance, enzyme target-object peridiumed with coupling substance of egg albumin and reaction production of QCA and gamma-aminobutyric acid. The sample is hydrolysed by metaphosphoric acid to discharge QCA, and cleaned by MAX column, derivatized by butyl amine, indirect competition ELISA is adopted in detection. The method and reagent box in the invention has advantages of simpleness, speediness, sensitivity and accuracy, large-lot samples can be detected fast and simultaneously, lowest detecting limit is 0.6 mug / kg.

The invention relates to a preparation method of a hollow polyethersulfone fiber ultrafiltration membrane capable of resisting biological pollution. The preparation method comprises the following steps: uniformly dispersing a modified material which is titanium dioxide / chitosan / graphene composite material in an organic solvent through ultrasounds, then adding a polyethersulfone material and an additive, adjusting the temperature of a membrane casting solution, uniformly stirring the solution and then standing the solution to obtain the membrane casting solution; and preparing the hollow fiber ultrafiltration membrane by using a dry-wet phase transformation spinning method, and adjusting the structure and the performance of the ultrafiltration membrane by controlling parameters such as temperatures and composition of a external gel bath and a core solution, working power of a metering pump, flow of the core solution, dragging speed of a membrane filament and the like. The water transmission coefficient of the prepared ultrafiltration membrane filament is greater than 150L.m<-2>.h<-1> under the pressure of 0.1MPa; the interception rate of egg albumin (45000) is greater than or equal to 90%; the flux recovery rate of the ultrafiltration membrane is greater than or equal to 60%; the antibacterial rate of escherichia coli is greater than or equal to 70%; the antibacterial rate of staphylococcus aureus is greater than or equal to 72%; the mechanical resistance intensity is greater than or equal to 4.5MPa. The ultrafiltration membrane prepared by adopting the preparation method is good in capability of resisting biological pollution and mechanical resistance and especially applicable to the fields of desalination pretreatment of seawater, brackish water, biochemical industry, pharmaceuticals and foods.

The invention provides a method for extracting protein from egg white. The method mainly uses a salt precipitation combined with resin adsorption to separate six proteins including ovomucin, ovotransferrin, egg albumin, egg inhibitor, egg mucin and lysozyme from egg white. The method is simple in process and short in extraction cycle and easy to realize industrialization, not only avoids the problem of toxic reagent residues, but also greatly improves production efficiency. The method greatly improves the added value of egg processing, and produces significant economic and social benefits.

The invention relates to a making method of a pork liver sausage. The making method is characterized by needing the following components in parts by mass: 5 parts of pork liver, 3 parts of ground meat, 2 parts of marbled meat, 0.01-0.2 part of table salt, 0.05-0.1 part of white granulated sugar, 0.05-0.1 part of monosodium glutamate, 0.01-0.03 parts of white pepper powder, 0.1 part of ginger powder, 0.1-0.15 part of five spice powder, 0.1-0.2 part of chilli powder, 0.1-0.2 part of chilli flakes, 0.01-0.03 part of onion powder, 0.02 part of dark soy sauce, 0.02-0.04 part of phosphate, 0.011 part of D-sodium erythorbate, 0.00005 part of sodium nitrite, 0.2-0.25 part of modified starch, 0.2-0.3 part of soy isolate protein, 0.05-0.08 part of carrageenan, 0.1-0.3 parts of egg albumin, and 1.8-2.3 parts of ice water. The making method comprises the following specific processes of: 1) salting the pork liver; 2) mincing the ground meat and marbled meat, wherein the ground meat is minced through a 6mm pore plate, and the marbled meat is minced through an 8mm pore plate; 3) mixing the materials; and 4) cooking and filling.

The invention belongs to the field of feed additives, and in particular relates to a pemix containing T4 lysozyme from T4 bacteriophage. The T4 lysozyme from the T4 bacteriophage replaces the traditional animal antibiotics, and the antibacterial growth-promoting feed premix containing the T4 lysozyme is prepared. A method for preparing the premix comprises the following steps of: centrifuging T4 lysozyme basic solution to remove impurities, performing membrane concentration, refining and purifying by using cation exchange resin, preparing protease powder by using a low-temperature spray drying method, screening the protease powder, adding auxiliary materials, and uniformly mixing at a high speed by a mixer to prepare the T4 lysozyme premix. The added auxiliary materials comprise soya beanmeal, bran, wheatmeal, wheat middling, bentonite, CaCO3, and zeolite. Experimental results show that the premix containing the T4 lysozyme has the effect of treating piglet diarrhea, which is obviously superior to that of a premix containing lysozyme from chicken egg albumin; and experimental results for feeding meat chickens and carp show that the premix containing the T4 lysozyme has the weightincreasing effect and feed utilization rate which are slightly superior to those of the premix containing lysozyme from chicken egg albumin.

The invention discloses an enzyme immune detecting agent box suit for analyzing the surplus furazolidone and its application in the field of chemo immunology analyzing technology. The agent box is mainly formed by a 3-amido-2-AOZ peculiarity antibody, a AOZ standard solution which comprises AOZ and the ferment standard plate of the egg albumin complex. The sample releases AOZ after alcaine hydrolysis, then the benzaldehyde derivatizes over the night, MAX column purifies, it adopts indirect contest ELISA method to detect the animal structure such as liver and the AOZ surplus in muscle. The core technology comprises: artificial antibody synthesis, antibody preparing, ELISA method establishing, and ELISA agent box adjustment.

The invention discloses a danofloxacin coupling compound with general formula (I), which comprises coupling compounds of danofloxacin hapten and carrier substance of bovine serum albumin which can produce immunogen or egg albumin. Thereinto, n stands for molecular number of danofloxacin combined with a bovine serum albumin molecule, the said n is an integer between one and twenty, and BSA stands for bovine serum albumin with 6.6KDa-6.9KDa of molecular weight. The invention also discloses a process for preparing the said coupling compound, which consists of joining danofloxacin and carrier substance which can produce immunogen to obtain the said coupling compound which induces immune system of animals to produce antibody. By immuning white rabbits from New Zealand, the danofloxacin coupling compound of this invention has prepared antiserum with 1:256,000 of potency, of which the lowest check limit is 0.01ppb. The invention is characterized in that it is simple, rapid, specific, exact and so on, which provides a foundation for preparing enzyme immunoassay agent box of danofloxacin.

A conjugate of tetracycline is prepared from tetracycline semi-antigen and the bovine serum albumin (or egg albumin) as the carrier able to generate immunogenicity through coupling. It can be used for preparing the reagent kit for the enzyme-linked immunoassay of tetracycline.

The invention discloses a method for synthesis of a chlorothalonil artificial antigen and belongs to the technical field of biochemical engineering. A chlorothalonil raw drug and 6-aminocaproic acid undergo a reaction to produce hapten CTNH as a hydroxy product, the CTNH is coupled with carrier protein bovine serum albumin (BSA) by a carbodiimide method so that a conjugate CTNH-BSA is obtained and is used as an immunizing antigen, the CTNH is coupled with carrier protein chicken egg albumin (OVA) so that a conjugate CTNH-OVA is obtained and is used as a coating antigen, and conjugate coupling ratios are determined by an ultraviolet method. The method realizes successfully synthesis of the chlorothalonil artificial antigen, has simple and effective synthesis steps, can be completely used for immunization analysis and provides a necessary artificial antigen for later research.

The invention discloses an application of a DGK alpha (diacylglycerol kinase alpha) gene-chitosan nanoparticle as a unique active component in preparing a medicine for treating allergic asthma as well as a preparation method of the DGK alpha gene-chitosan nanoparticle. The invention, aiming at shortcomings that naked DGK alpha plasmid is easy to be damaged by nuclease, acid, alkali and the like in the body, prepares the DGK alpha gene-chitosan nanoparticle through chitosan, so as to effectively protect the DGK alpha plasmid, enhance the activity of the DGK alpha plasmid in the body and promote the DGK alpha gene to develop better function in the body. The DGK alpha, based on a function of induction of T cell anergy, can induce immune tolerance. The DGK alpha gene-chitosan nanoparticle prepared by the invention can inhibit egg albumin-induced airway inflammation of asthmatic mice, reduce serum egg albumin specific IgE level, and is expected to be used for treating asthma and other allergic diseases.

The invention relates to a synthetic method of dimethoxy phosphate ester type pesticide universal hapten, and belongs to the technical field of the biochemical engineering. The synthetic process of the invention comprises three steps of esterification, acylation and hydrolysis. Firstly, para hydroxybenzene propionic acid and carbinol have esterification reaction to make carboxy metho esterified; and then the obtained para hydroxybenzene methyl propionate has reaction with dimethoxy phosphorus oxychloride to obtain dimethoxy phosphate ester; finally the methyl ester formed in the first step is hydrolyzed again, and carboxyl is reduced to obtain O, O-dimethyl-O-(4-propionyloxy phenyl group) phosphate ester, and the phosphate ester is coupled with egg albumin to be the envelope antigen separated out by redundant ELISA of the dimethoxy phosphate ester type pesticide. The substance is used as the envelope antigen during the process of enzyme linking immunity checking of the pesticide after being coupled with the albumen, and the structure has benzene ring, thereby using the difference of envelope antigen and the immunity primary structure to further improve the checking sensitivity and reduce the checking limitation.

The invention provides a colloidal gold quenching quantum dot immunochromatography test strip for detecting zearalenone, comprising a sample pad 1, a nitrocellulose membrane 2, a water-absorbing pad 3 and a PVC backboard, characterized in that, A sample pad 1, a nitrocellulose membrane 2, and a water-absorbing pad 3 are adhered to the PVC backboard in sequence; the nitrocellulose membrane 3 is coated with zearalenone antigen and quantum dot-chicken ovalbumin respectively. The detection line 4 formed by the conjugate and the quality control line 5 formed by the quantum dot-chicken ovalbumin conjugate. The invention also discloses a preparation method of the test strip. The invention has the following prominent advantages: 1. High specificity and good sensitivity; 2. Low detection cost. 3. Easy to operate.

The invention discloses a nougat and a preparation method thereof, comprising the following raw materials in parts by weight: 24-26 parts of oats, 30-32 parts of sorghum, 12-14 parts of maltose, 7-9 parts of butter, and 8 parts of lactose ‑12 parts, 1‑3 parts of mint, 0.6‑0.8 parts of vanillin, 4‑5 parts of egg protein, 5‑7 parts of low-fat milk powder, appropriate amount of water, through the selection and preparation of oats and sorghum, and the preparation of mint leaves , preparation of foaming agent, preparation of inflated sugar base, boiling sugar, mixing, cooling and forming, the nougat is rich in phosphorus, iron, calcium, B vitamins, niacin, folic acid, pantothenic acid and many A kind of amino acid, which has the medical value and health care function of improving blood circulation, lowering blood pressure, lowering cholesterol, preventing and treating colorectal cancer, preventing and treating heart disease, benefiting the spleen and warming the middle, astringing the intestines and stopping diarrhea.

The invention discloses a preparation method of heavy metal cadmium artificial antigen, which applies 2-S-(4- aminobenzene)-1, 4, 7, 10 tetraazacyclo cyclononane-1, 4, 7, 10-acetate tetrahydrate(p-NH2-Bn-DOTA, DOTA for short) as cheating agent, and couples cadmium ion chelating agent composite with carrier protein bovine serum albumin BSA or egg albumin OVA; thus the artificial antigen is prepared. The method adds the sodium borohydride processing step on the basis of traditional method, and improves the coupling efficiency and antiserum titre. The invention further discloses an application ofDOTA in preparation of the heavy metal cadmium artificial antigen.

The invention belongs to the field of biomedicine, relating to a method for establishing a chronic obstructive pulmonary disease superposition asthma model. By adopting a combined method of Drip endotoxin and smoke in trachea and egg albumin sensitization inspiration, the invention has reliable evaluation on aspects of lung tissue pathology, bronchial reactivity, serum cytokine, hypothalamus-hypophysis-adrenal axis and the like. The result of comparison with the pure chronic obstructive pulmonary disease or asthma model shows that the chronic obstructive pulmonary disease superposition asthma model has the characteristic change of the chronic obstructive pulmonary disease or asthma and weakens or intensifies the charateristic pathologic physiological changes. The invention has the advantages of simple preparation and good reliability and repetitiveness, and meets the disease pathologic physiological characteristics of the chronic obstructive pulmonary disease merger asthma, and can be used for the research of pathogenic mechanisms, prevention and treatment, and the like of the chronic obstructive pulmonary disease merger asthma.

The invention discloses a processing method capable of improving pagrosomus major surimi elasticity and preventing fish protein frozen denaturation. The principle of the processing method comprises that frozen egg white or albumen powder and other food additives are added into pagrosomus major surimi and the mixture is subjected to high speed stirring and atomization. The adopted frozen egg white comprises frozen chicken egg white and frozen duck egg white. The adopted albumen powder comprises chicken albumen powder and duck albumen powder. The processing method realizes two purposes that 1, surimi elasticity is improved, and concretely, egg white has an irreversible coagulation capability and when egg albumin and original fish proteins are partly combined, a cysteine protease inhibitor component in egg white can inhibit fish protein degradation, guarantee fish protein content, and improve a surimi coagulation capability; and 2, a protein chain is shortened through stirring emulsification and atomization treatment, and thus protein aggregation denaturation caused by influences from ice crystals is difficult to occur.

The invention relates to a biological active substance separating and purifying technology, and specifically relates to a method for two-aqueous phase extraction of abalone egg albumin polypeptide concentrated liquor. The method specifically comprises the following steps: prepreparing abalone egg homogenate, separating and purifying after two-aqueous phase continuous extraction, wherein total density of a two-aqueous phase system is 0.06kg / m<3>-0.16kg / m<3>, viscosity is 4mpa / s-16mpa / s, pH value is 6-8, extraction time is 30 minutes-50 minutes, and the feeding proportion of the total amount of the two-aqueous phase system to the abalone egg homogenate is 1: (0.4-0.5). The method disclosed by the invention is short in phase separating time, saves extraction time, saves energy consumption, and recycles distribution base liquor after phase separation, does not have organic solvent residue; moreover, the abalone egg albumin polypeptide concentrated liquor and nutritious foods are widely combined and applied.

The invention discloses a pefloxacin coupling compound with general formula (I), which comprises coupling compounds of pefloxacin hapten and bovine serum albumin of carrier substance which can produce immunogen or egg albumin. Thereinto, n stands for molecular number of pefloxacin combined with a bovine serum albumin molecule, the said n is an integer between one and twenty, and BSA stands for bovine serum albumin with 6.6KDa-6.9KDa of molecular weight. The invention also discloses a process for preparing the said coupling compound, which consists of joining pefloxacin and carrier substance which can produce immunogen to obtain the said coupling compound which induces immune system of animals to produce antibody. By immuning white rabbits from New Zealand, the pefloxacin coupling compound of this invention has prepared antiserum with 1:512,000 of potency, of which the lowest check limit is 0.01ppb. The invention is characterized in that it is simple, rapid, specific, exact and so on, which provides a foundation for preparing enzyme immunoassay agent box of pefloxacin.