The invention belongs to fields of
immunochemistry analysis. It discloses an
enzyme-uniting immune method and
reagent box used in detection of
quinoxaline-2-carboxyl acid residues, the method mainly contain
medicine reconstruction, the preparation of
immunogen,
peridium antigen and
antibody and pretreatment of sample and building of ELISA detecting method. The
reagent box comprises
quinoxaline-2-carboxyl acid (QCA) specificity
antibody, QCA standard substance,
enzyme target-object peridiumed with
coupling substance of
egg albumin and reaction production of QCA and gamma-
aminobutyric acid. The sample is hydrolysed by
metaphosphoric acid to
discharge QCA, and cleaned by MAX column, derivatized by butyl amine, indirect competition ELISA is adopted in detection. The method and
reagent box in the invention has advantages of simpleness, speediness, sensitivity and accuracy, large-lot samples can be detected fast and simultaneously, lowest detecting limit is 0.6 mug / kg.