37 results about "Immunochemistry assay" patented technology

This invention discloses one enzyme immune test method and agent case for 3- methyl quinoline 2-carboxyl acid in immune chemical analysis technique field, which comprises the following steps: processing immune antigen and cover antigen and alloantibody and pre-processing sample and establishing ELISA method; the matched agent case is composed of 3- methyl quinoline 2-carboxyl acid alloantibody covered by MQCA and egg albumin couple and MQCA standard sample; the sample is processed through metaphosphoric acid to release MQCA and MAX and indirectly competition ELISA method.

The invention relates to a preparation method of a benzothiostrobin antigen and antibody and an application thereof, belonging to the technical field of immunochemical analysis. The chemical name of benzothiostrobin is 2-[[(5-methoxy-2-benzothiazole)-thiomethyl]-alpha-(E)-methoxymethylene]methyl phenylacetate. Under an alkaline condition, carboxylic ester in the benzothiostrobin structure is hydrolyzed to synthesize an artificial hapten of which the chemical name is 2-[[(5-methoxy-2-benzothiazole)-thiomethyl]-alpha-(E)-methoxymethylene]phenylacetic acid; the artificial hapten is coupled with bovine serum albumin and ovalbumin respectively to prepared an artificial antigen. The BALB / c female mouse is immunized by the artificial antigen to obtain a specific monoclonal antibody of benzothiostrobin. The antibody does not experience a cross reaction with other compounds. An enzyme linked immunosorbent assay method established by use of the antibody can be applied to quick, sensitive, convenient and cheap detection of benzothiostrobin residues in environment and agricultural products.

The invention relates to a clothianidin antigen, an antibody and an application thereof, and belongs to the technical field of immunochemistry analysis. The clothianidin antigen and the antibody of the invention are specially used for clothianidin specific polyclonal antibody preparation, enzyme-linked immunoassay (ELISA), and high-sensitivity and rapid detection of clothianidin residues in environment and agricultural products. A hapten is synthesized by substituting a chlorine atom on a thiazole ring of clothianidin, has a chemical name of 3-(5-((3-methyl-2-nitroguanidine)-yl methyl)thiazole-2-mercapto)propionic acid, and is coupled with bovine serum albumin and ovalbumin to prepare an antigen and an envelope antigen. Newzealand white rabbit is immunized by the immunizing antigen to obtain the specific polyclonal antibody of clothianidin. The established ELISA linear scope is 1.1 microgram / L-2 mg / L, and the detection limit is 1.1 microgram / L. The hapten synthetic technology of the invention is simple and feasible, high in antibody specificity, and suitable for detection and on-site monitoring of mass samples in environment and agricultural products.

Hydrophilic, chemiluminescent acridinium compounds containing zwitterions are disclosed. These acridinium compounds, when used as chemiluminescent labels in immunochemistry assays and the like, exhibit decreased non-specific binding to solid phases and provide increased assay sensitivity.

The invention belongs to fields of immunochemistry analysis. It discloses an enzyme-uniting immune method and reagent box used in detection of quinoxaline-2-carboxyl acid residues, the method mainly contain medicine reconstruction, the preparation of immunogen, peridium antigen and antibody and pretreatment of sample and building of ELISA detecting method. The reagent box comprises quinoxaline-2-carboxyl acid (QCA) specificity antibody, QCA standard substance, enzyme target-object peridiumed with coupling substance of egg albumin and reaction production of QCA and gamma-aminobutyric acid. The sample is hydrolysed by metaphosphoric acid to discharge QCA, and cleaned by MAX column, derivatized by butyl amine, indirect competition ELISA is adopted in detection. The method and reagent box in the invention has advantages of simpleness, speediness, sensitivity and accuracy, large-lot samples can be detected fast and simultaneously, lowest detecting limit is 0.6 mug / kg.

The invention relates to an artificial antigen and antibody that is used for analyzing sulfa plural residual immunity and the manufacturing method. It uses 4-(4- amino benzene sulfonyl) benzoic acid as hapten and compound artificial antigen with carrier protein and taking animal immunity to make antibody that could be used to rapidly test the residual to sulfa. It has good broad spectrum feather and has great application prospect.

The method comprises: synthesizing the hapten 2-methyl-3-phenyl benzyl succinic acid ester (for short: LBC) and the 2-methyl-3-phenylbenzene benzoic acid (for short: LBY); coupling the LBc with protein to prepare the artificial immunity antigen and immunity rabbit to the specificity polyclonal antibody of Bifenthrin; coupling the LBy with the protein to prepare the envelope antigen; using the synthesized envelope antigen and specificity antibody to establish the enzyme immunoassay EIA analysis method; the detection range of the Bifenthrin can reach 0.016mg / L, and the linear range of it is 0.01-50mg / kg.

A method for modification of solid substrates with proteins for efficient surface preconcentration of an analyte from multi-component samples before the detection based on desorption-ionization mass spectrometry and immunochemical assays. The claimed subject is a method of modification of surfaces used as substrates for desorption-ionization mass spectrometry and immuno-chemical assays. The method is based on electronebulization (electrospraying) of protein solution, depending on the intended application either enzymes, lectins, or antibodies. The formed charged electrospray is dried in real time by its passing through an evaporation compartment and the resulting beam of desolvated ions impacts onto the surface and binds to it firmly. Such modified surface can be then used for a selective interaction with an affinity partner of the deposited protein, its preconcentration or enzymatic modification followed by an analysis by means of desorption-ionization mass spectrometry or immunochemical assays.

Aspects of the present disclosure relate to a method and / or a device for carrying out chemical, biochemical and / or immunochemical analyses of liquid samples, which are present in a sample store of an automatic analyzer, with the aid of liquid reagents which are present in at least one reagent store of the analyzer. In one example embodiment, the automatic analyzer includes cuvettes, a first pipettor, a device with an optical measurement unit, a device for heterogenous immunoassays, a cuvette washing unit, a needle washing unit, a temperature control unit.

The invention discloses an enzyme immune detecting agent box suit for analyzing the surplus furazolidone and its application in the field of chemo immunology analyzing technology. The agent box is mainly formed by a 3-amido-2-AOZ peculiarity antibody, a AOZ standard solution which comprises AOZ and the ferment standard plate of the egg albumin complex. The sample releases AOZ after alcaine hydrolysis, then the benzaldehyde derivatizes over the night, MAX column purifies, it adopts indirect contest ELISA method to detect the animal structure such as liver and the AOZ surplus in muscle. The core technology comprises: artificial antibody synthesis, antibody preparing, ELISA method establishing, and ELISA agent box adjustment.

The invention relates to analytical chemistry and to quantitative immunochemical analysis, in particular, to a method for immunochemical quantitative detection of various biological toxins by using biological microchips. A biological microchip comprises an ordered array of three-dimensional hydrogel cells on a solid support, which are produced by a method of photo- or chemically induced polymerization and contain immobilized antibodies to various bacterial, plant and animal biotoxins, or biotoxins. The use of microchips makes it possible to analyze a sample simultaneously for the presence of several biotoxins. The proposed method for detecting biotoxins can be used in medicine, in food industry, and in environmental protection.

An improved extractive reagent composition and method for extracting an immunosuppressant drug, such as sirolimus, tacrolimus or cyclosporine, from blood samples while yielding a test sample extract that has low vapor pressure and is compatible with immunoassay components. The inventive reagent composition comprises dimethyl sulfoxide (DMSO), at least one divalent metal salt and water. The sample extracts resulting from use of each of these combinations have low vapor pressure and are compatible with immunochemistry assays.

The invention discloses the preparation of a monoclonal antibody and application thereof, and in particular relates to the preparation of a monoclonal antibody capable of synchronously detecting residues of Sudan I and para red and application thereof, which belong to the technical field of immunochemistry analysis. A complex formed by crosslinking a Sudan red derivative and bovine serum albumin is taken as immune antigen, the immune antigen is used to immunize a Balb / C mouse, sensitized spleen cells and myeloma cells SP2 / 0 of the mouse are fused to prepare hybridoma cells, a monoclonal hybridoma cell strain is obtained through an indirect ELISA screening method and a limited dilution method, and the hybridoma cell strain is injected into an abdominal cavity of the mouse to produce the monoclonal antibody. The invention provides an antigen prepared based on the method and an enzyme-linked immunoassay (ELISA) method that the characteristics of the monoclonal antibody are suitable to qualitatively and quantificationally detect the Sudan I, the para red and the content of the Sudan I and the para red.

Disclosed herein are instruments, systems and methods for performing automated integrated analysis of both clinical chemistry assay and immunoassay tests on a sample. The system includes a common process subsystem module; a clinical chemistry analyzer module; an immunoassay analyzer module; and a plurality of additional modules. The common process subsystem module is configured to position one or more reaction vessels containing aliquots of the sample for analysis by the clinical chemistry analyzer module, the immunochemistry analyzer module or both analyzer modules. The included immunochemistry analyzer module of the instrument and system is configured to perform multiplex FRET analysis on homogeneous solutions, thereby increasing the flexibility, throughput and robustness of the resultant instrument and systems.

The invention discloses a decamethrin antigen, an antibody and application thereof, which belong to the technical field of immune chemical analysis, and provides a method special for preparing decamethrin specific antibody and detecting enzyme coupled immunity. The method comprises the following steps: synthesizing hapten 1-carboxyl-(3'-phenoxyl phenyl) methyl-3-(2', 2'-dibromo vinyl)-2, 2-dimethyl cyclopropyl carboxylic ester (Med for short) and N-2-(carboxyl propyl) carbamoyl-(3'-phenoxyl phenyl) methyl-3-(2', 2'-dibromo vinyl)-2, 2-dimethyl cyclopropyl carboxylic ester (Di for short); preparing artificial immune antigen by coupling the Di and protein; obtaining specific polyclonal antibody of decamethrin by an immune rabbit; preparing envelope antigen by coupling the Med and the protein; and finally establishing an enzyme coupled immunity analytic method by using the synthesized envelope antigen and the specific antibody, wherein the detection limit of the decamethrin reaches 0.004 milligram per liter, the linearity is between 0.005 and 100 milligrams per kilogram, and the decamethrin antigen has no remarkable cross reaction for most pyrethroid and metabolite thereof.

This invention relates to one cypermethrin human antigen process method in immune chemical analysis technique field, which comprises chrysanthemumic acid into alcohol liquid for centrifugation and taking for upper clear liquid for use; then taking cow blood serum protein and carbodiimide into sterilized water; then dripping by mixing into the protein liquid with chrysanthemumic acid and carbodiimide liquid for light shield reaction in ice; dripping remaining carbodiimide liquid into the above mixture liquid for continuing shield reaction; through centrifugation to take dialysis through cooling to get human antigen.

A method of analyzing a sample for one or more analytes of interest includes: providing an element having a base layer; a layer containing streptavidin; a spreading layer, wherein the streptavidin may or may not be in the spreading layer; providing immunocomponents and labeled immunoreactants which may be in the spreading layer or may be combined with the sample; dispensing the sample, and optionally the immunocomponents and a labeled immunoreactant onto the spreading layer at three or more areas, wherein each center of the three or more areas is substantially equidistance to the center formed by the three or more areas, and wherein each area contacts an adjacent area such that wash fluid flow in any direction will contact sample; washing the sample and label by directing the wash fluid at the center formed by the three or more areas, whereby the wash fluid equally flows over each of the three or more areas; and taking a measurement at each three or more areas to determine the presence or concentration of the one or more analyte. In a preferred embodiment, the element is a dry slide used on a diagnostic analyzer.

The invention relates to haptens, immunogens and secondary immunoreactive agents, to the use thereof for producing wide-spectrum antibodies against quinolone-type antibiotics, to the application thereof to immunochemical analysis techniques, and to a kit enabling the detection of said antibiotics in biological samples from food products of animal origin.

The invention discloses an artificial antigen and antibody for immunoassay of melamine residues and a preparation method thereof, belonging to the immunochemical analysis technology of small-molecular compounds. According to the invention, 4,6-diamino-1,3,5-triazine-2-acetic acid is directly used as a hapten, and the hapten is combined with bovine serum albumin or ovalbumin by an active ester method to obtain a melamine artificial antigen; and an antibody is prepared through animal immunization, wherein the antibody has good specificity to melamine, and a foundation is laid for an immunological detection method for quickly and accurately detecting melamine residues in food.

The invention relates to an artificial antigen and antibody that is used for analyzing sulfa plural residual immunity and the manufacturing method. It uses 4-(4- amino benzene sulfonyl) benzoic acid as hapten and compound artificial antigen with carrier protein and taking animal immunity to make antibody that could be used to rapidly test the residual to sulfa. It has good broad spectrum feather and has great application prospect.

The invention discloses an L-lauramide arginine monohydrochloride ethanol ester artificial antigen and a specific antibody as well as a preparation method and application thereof and belongs to the technical field of immunochemical analysis. The antigen is specially used for preparation of an L-lauramide arginine specific antibody and an enzyme-linked immunosorbent assay method, artificial antigens LAS-BSA and LAS-OVA are synthesized, an immune rabbit obtains an L-lauramide arginine specific polyclonal antibody, and finally an enzyme-linked Immunoassay method is established by utilizing the synthesized coating antigen and the specific antibody. The method has the lowest LAE detection limit of 0.005mg / L, recovery is 82.4-109.8%, the average variation coefficient is 3.2-5.8%, and the method has the characteristics of high accuracy and high sensitivity and accords with residue analysis standard.

Methods and apparatus that mix a plurality of individual capture reagents for the diagnostic assays are described herein. In an embodiment, a system for optically analyzing a patient sample includes an automated immunochemistry analyzer storing a plurality of capture reagents and a plurality of paramagnetic particles, a user interface configured to allow a selection of a combination of two or more of the capture reagents, and a logic implementer configured to cause the automated immunochemistry analyzer to (i) mix together each capture reagent of the combination of two or more of the capture reagents; (ii) bind the mixture of the combination of two or more of the capture reagents to the paramagnetic particles; (iii) bind the patient sample to the bound mixture of the combination of two or more of the capture reagents; and (iv) optically analyze the patient sample.

The invention relates to the technical field of immunochemical analysis and pesticide residue detection, in particular to a hapten of an organophosphorus triazophos pesticide and a preparation method of the hapten. According to the preparation method, 1-phenyl-3-hydroxy-1, 2, 4-triazole serves as a raw material, and the hapten of the organophosphorus triazophos pesticide is synthesized through nitration reaction, substitution reaction, reduction reaction and carboxyl active group introduction reaction. Therefore, the antibody with high specificity and high affinity to organophosphorus pesticides can be prepared.

The invention relates to haptens, immunogens and secondary immunoreactive agents, to the use thereof for producing wide-spectrum antibodies against quinolone-type antibiotics, to the application thereof to immunochemical analysis techniques, and to a kit enabling the detection of said antibiotics in biological samples from food products of animal origin.

The invention discloses trinexapac-ethyl antigens, a specific antibody and uses of the antibody, and belongs to the technical field of immunochemical analysis. The antigens are specially used for preparation of the trinexapac-ethyl specific antibody and an enzyme-linked immunological detection method. The artificial antigens which are TNc-BSA and TNc-OVA are synthesized, a rabbit is immunized to obtain the trinexapac-ethyl specific polyclonal antibody, and finally an enzyme-linked immunosorbent assay method is established by utilizing the synthesized coated antigens and the specific antibody. The lowest trinexapac-ethyl detection limit of the method is 0.005 mg / L, the recovery rate is 85.2-103.1%, and the average coefficient of variability is 2.1-7.9% antibody, thus meeting residue analysis standards.

The invention relates to a preparation method of a benzothiostrobin antigen and antibody and an application thereof, belonging to the technical field of immunochemical analysis. The chemical name of benzothiostrobin is 2-[[(5-methoxy-2-benzothiazole)-thiomethyl]-alpha-(E)-methoxymethylene]methyl phenylacetate. Under an alkaline condition, carboxylic ester in the benzothiostrobin structure is hydrolyzed to synthesize an artificial hapten of which the chemical name is 2-[[(5-methoxy-2-benzothiazole)-thiomethyl]-alpha-(E)-methoxymethylene]phenylacetic acid; the artificial hapten is coupled with bovine serum albumin and ovalbumin respectively to prepared an artificial antigen. The BALB / c female mouse is immunized by the artificial antigen to obtain a specific monoclonal antibody of benzothiostrobin. The antibody does not experience a cross reaction with other compounds. An enzyme linked immunosorbent assay method established by use of the antibody can be applied to quick, sensitive, convenient and cheap detection of benzothiostrobin residues in environment and agricultural products.