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Artificial antigen used for immune analysis of sulfanilamide multi kind residue and antibody and its preparation

An immunoassay, artificial antigen technology, applied in analytical materials, material testing products, measuring devices, etc., to achieve the effect of easy popularization, simple synthesis method and good specificity

Inactive Publication Date: 2007-01-31
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, artificial antigens with characteristic structures of sulfonamide polydrugs and specific antibodies against sulfa polydrugs have not been reported yet.

Method used

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  • Artificial antigen used for immune analysis of sulfanilamide multi kind residue and antibody and its preparation
  • Artificial antigen used for immune analysis of sulfanilamide multi kind residue and antibody and its preparation
  • Artificial antigen used for immune analysis of sulfanilamide multi kind residue and antibody and its preparation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1. Hapten Synthesis

[0043] Dissolve 2 mmol of 4-acetylaminobenzenesulfonyl chloride in 5 mL of dry pyridine, N 2 Under protection, 6 mL of a dry pyridine solution containing 2 mmol of 4-aminobenzoic acid was added dropwise, and magnetically stirred. After the dropwise addition, the reaction mixture was refluxed at 90°C for 3 h, and the progress of the reaction was monitored by TLC, followed by the addition of 20 mL of 2 mol·L -1 NaOH, pyridine was removed under reduced pressure. The reaction system was refluxed for another 2 h, and the reaction progress was monitored by TLC, then cooled to 15°C, and the pH value was adjusted to 3.5 with concentrated hydrochloric acid. The product was extracted 3 times with ethyl acetate, 20 mL each time, the organic phases were combined and washed with anhydrous Na 2 SO 4 Dry and remove solvent under reduced pressure. Purify the residue through a 200-300 mesh silica gel column to obtain the target hapten 4-(4-aminobenzenesulfonyl...

Embodiment 2

[0054] 1. The specific method of hapten synthesis is:

[0055] 2.12 mmol of 4-aminobenzoic acid was dissolved in 6 mL of dry pyridine solution, 2.28 mmol of 4-acetylaminobenzenesulfonyl chloride was dissolved in 5 mL of dry pyridine, N 2 Under protection, it was added dropwise into the 4-aminobenzoic acid solution and stirred by magnetic force. After the dropwise addition, the reaction mixture was refluxed at 95°C for 2h, followed by the addition of 2mol L -1 NaOH 25mL, pyridine was removed under reduced pressure. The reaction system was refluxed for another 2.5 hours, cooled to 20°C, and the pH value was adjusted to 4.0 with concentrated hydrochloric acid. The product was extracted with ethyl acetate (3×30 mL), the organic phases were combined and washed with anhydrous Na 2 SO 4 Dry and remove solvent under reduced pressure. The residue was purified through a silica gel column (200-300 mesh) to obtain the target product;

[0056] 2. The specific method of artificial ant...

Embodiment 3

[0064] 1. Hapten Synthesis

[0065] Dissolve 3.5 mmol 4-acetylaminobenzenesulfonyl chloride in 5 mL dry pyridine, N 2 Under protection, 6 mL of a dry pyridine solution containing 3 mmol of 4-aminobenzoic acid was added dropwise, and magnetically stirred. After the dropwise addition, the reaction mixture was refluxed at 85°C for 3 h, and the progress of the reaction was monitored by TLC, followed by the addition of 30 mL of 2 mol L -1 NaOH, pyridine was removed under reduced pressure. The reaction system was refluxed for another 3 h, and the reaction progress was monitored by TLC, then cooled to 25° C., and the pH value was adjusted to 4.5 with concentrated hydrochloric acid. The product was extracted 3 times with ethyl acetate, 40 mL each time, the organic phases were combined and washed with anhydrous Na 2 SO 4 Dry and remove solvent under reduced pressure. Purify the residue through a 200-300 mesh silica gel column to obtain the target hapten 4-(4-aminobenzenesulfonyl)b...

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Abstract

The invention relates to an artificial antigen and antibody that is used for analyzing sulfa plural residual immunity and the manufacturing method. It uses 4-(4- amino benzene sulfonyl) benzoic acid as hapten and compound artificial antigen with carrier protein and taking animal immunity to make antibody that could be used to rapidly test the residual to sulfa. It has good broad spectrum feather and has great application prospect.

Description

technical field [0001] The invention belongs to the technical field of immunochemistry and residual analysis of small molecule compounds (molecular weight less than 1000 Daltons); relates to organic synthesis, immunochemistry, biochemistry and physical and chemical testing techniques; in particular to sulfonamides with the molecular structure of p-aminobenzenesulfonamide Design and synthesis of artificial haptens and artificial antigens of small molecular compounds, and preparation of specific antibodies for immunized animals. Background technique [0002] Sulfonamide antibacterial drugs are a class of small molecular compounds that inhibit nucleic acid synthesis. In the feeding of food-borne animals, they are widely used as veterinary drugs for the prevention and treatment of bacterial infectious diseases. They are currently one of the most widely used and most used veterinary drugs. , Also used as animal feed additive. Studies have shown that sulfonamides can inhibit bone...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/531
Inventor 王硕张燕段振娟张鸿雁
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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