The invention relates to a high-precision
nucleic acid quantitative detection kit for
hepatitis C
virus (HCV) applied to the field of biomedical
clinical diagnosis. The kit comprises a
nucleic acid extraction kit based on a paramagnetic
particle method and an HCV
nucleic acid amplification box, wherein the nucleic acid extraction kit based on the paramagnetic
particle method comprises a splitting combining liquid, a rinsing liquid A, a rinsing liquid B, a rinsing liquid C, an eluting liquid and a
magnetic bead liquid. The HCV nucleic acid amplification box comprises an RT-PCR (Reverse Transcription-
Polymerase Chain Reaction) reaction liquid,
enzyme mixed liquor, an HCV-interior
label, an HCV quantitative reference 1-4, a negative
quality control product, a critical positive
quality control product and a positive
quality control product. The splitting combining liquid in the nucleic acid extraction agent based on the paramagnetic
particle method comprises 0.5-2.5% of lauryl
sodium sulfate, 0.5-2.0ml / 100ml of TritonX-100, 2-6mol / L of guanidinium
isothiocyanate and 1-10mM of EDTA (
Ethylene Diamine Tetraacetic Acid) (PH 7.5). The kit provided by the invention is simple and fast to operate, low in cost, high in coverage of primer and probe
genotype, high in conservative property, strong in specificity, high in detection sensitivity and good in
repeatability, and can better simulate the extraction state of real viruses.