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Method for preparing fenpropathrin artificial antigen

A technology of artificial antigen and fenothrin, which is applied in the direction of biological testing, material testing products, etc., can solve the problems of low productivity, cumbersome method, poor specificity, etc., and achieves the effect of simple operation and overcoming cumbersomeness.

Inactive Publication Date: 2007-08-15
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the above disadvantages, thereby providing a preparation method of fenpropathrin artificial antigen, which uses carbodiimide to couple fenpropathrin and bovine serum albumin to construct an artificial binding antigen; The method is easy to operate, completes the reaction in one step, overcomes the shortcomings of other methods such as cumbersome, low productivity, and poor specificity, and lays the foundation for the establishment of immunological analysis techniques for fenpropathrin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation method of fenpropathrin artificial antigen of the present invention adopts following processing steps:

[0022] Weigh 50mg of fenpropathrin [Fenpropathrin, (RS)-a-cyano-3-phenoxybenzyl 2,2,3,3-tetramethylcyclopropanecarboxylate], dissolve in 2mL 25% (weight percentage) in ethanol solution, dissolve for 4 hours and then centrifuge (800 type centrifugal precipitator produced by Shanghai Jingke Industrial Co., Ltd.), centrifugal speed: 4000r / min, centrifugation time: 10min, take the supernatant and set aside. Then take 100mg of bovine serum albumin (BSA) and 50mg of carbodiimide (EDC), dissolve them in 5mL and 3mL of sterile water respectively, and slowly add 2mL of fenpromethrin dropwise to the bovine serum albumin-BSA solution while stirring. The acid supernatant and 2 mL of carbodiimide (EDC) solution were reacted in an ice bath at 4°C for 6 h in a dark place, and then the remaining 1 mL of carbodiimide (EDC) solution was added dropwise to the above mixt...

Embodiment 2

[0030] The preparation method of fenpropathrin artificial antigen of the present invention adopts following processing steps:

[0031] Weigh 100mg of fenpropathrin [Fenpropathrin, (RS)-a-cyano-3-phenoxybenzyl 2,2,3,3-tetramethylcyclopropanecarboxylate], dissolve in 3mL of 25% ethanol solution, centrifuged after dissolving for 4 hours, and took the supernatant. Take 200mg of ovalbumin (OVA) and 100mg of carbodiimide (EDC), dissolve them in 8mL and 4mL of sterile water respectively, and slowly add 3mL of fenprochrysanthemic acid dropwise to the ovalbumin (OVA) solution while stirring The supernatant and 3 mL of carbodiimide (EDC) solution were reacted in a dark place in an ice bath at 4°C for 6 hours, and then the remaining 1 mL of carbodiimide (EDC) solution was added dropwise to the mixture, and placed in an ice bath at 4°C. Continue the shading reaction for 24h. After the reaction, centrifuge at 4000r / min for 10min, take the supernatant in a dialysis bag, dialyze in 0.01mol...

Embodiment 3

[0033] The preparation method of fenpropathrin artificial antigen of the present invention adopts following processing steps:

[0034] Weigh 50mg of fenpropathrin [Fenpropathrin, (RS)-a-cyano-3-phenoxybenzyl 2,2,3,3-tetramethylcyclopropanecarboxylate], dissolve in 2mL of 25% ethanol solution, centrifuged after dissolving for 4 hours, and took the supernatant. Take 100mg of bovine serum albumin (BSA) and 100mg of carbodiimide (EDC), dissolve them in 5mL and 3mL of sterile water respectively, and slowly add 2mL of formazan to the bovine serum albumin (BSA) solution while stirring. The supernatant of chrysanthemic acid and 2 mL of carbodiimide (EDC) solution were reacted for 6 hours in an ice bath at 4°C in a dark place, and then the remaining 1 mL of carbodiimide (EDC) solution was added dropwise to the mixture, and kept on ice at 4°C. Continue the shading reaction under the bath for 24h. After the reaction, centrifuge at 4000r / min for 10min, take the supernatant in a dialysis...

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Abstract

This invention relates to one cypermethrin human antigen process method in immune chemical analysis technique field, which comprises chrysanthemumic acid into alcohol liquid for centrifugation and taking for upper clear liquid for use; then taking cow blood serum protein and carbodiimide into sterilized water; then dripping by mixing into the protein liquid with chrysanthemumic acid and carbodiimide liquid for light shield reaction in ice; dripping remaining carbodiimide liquid into the above mixture liquid for continuing shield reaction; through centrifugation to take dialysis through cooling to get human antigen.

Description

technical field [0001] The present invention relates to a kind of preparation method of fenpropathrin artificial antigen, specifically utilize carbodiimide method, make fenpropathrin [Fenpropathrin, (RS)-a-cyano-3-phenoxybenzyl 2 , 2,3,3-Tetramethylcyclopropanecarboxylate] a very important precursor substance in the production process fenchroic acid (2,2,3,3-tetramethylcyclopropanecarboxylic acid) and The invention relates to a method for preparing fenpropathrin artificial antigen through direct cross-linking of carrier molecules (proteins), belonging to the technical field of immunochemical analysis. Background technique [0002] In the field of immunology, in order to make non-immunogenic small molecular substances (often called haptens, Hapten) induce antibodies in animals, it is often necessary to combine this hapten with a certain macromolecular carrier such as protein or polypeptide. After being cross-linked and combined to become an artificial antigen, it is then inj...

Claims

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Application Information

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IPC IPC(8): G01N33/53
Inventor 吴伟杨健瞿建宏刘洪波
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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