932results about How to "Reduce analysis costs" patented technology

A device for pre-concentration and purification of analytes from biological samples, such as human serum, to be analyzed by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI MS) and methods of use thereof are provided.

A multiple function atmospheric pressure ion source interfaced to a mass spectrometer comprises multiple liquid inlet probes configured such that the sprays from two or more probes intersect in a mixing region. Gas phase sample ions or neutral species generated in the spray of one probe can react with reagent gas ions generated from one or more other probes by such ionization methods as Electrospray, photoionization, corona discharge and glow discharge ionization. Reagent ions may be optimally selected to promote such processes as Atmospheric Pressure Chemical Ionization of neutral sample molecules, or charge reduction or electron transfer dissociation of multiply charged sample ions. Selected neutral reagent species can also be introduced into the mixing region to promote charge reduction of multiply charged sample ions through ion-neutral reactions. Different operating modes can be performed alternately or simultaneously, and can be rapidly turned on and off under manual or software control.

SNAIL provides cost-efficient detection of specific nucleic acids in single cells, and may be combined with flow cytometry to simultaneously analyze large numbers of cells for a plurality of nucleic acids, e.g. at least one, to up to 5, up to 10, up to 15, up to 20 or more transcripts can be simultaneously analyzed, at a rate of up to about 50, 100, 250, 500 or more cells / second. The methods require only two primers for amplification, and may further include a detection primer.

A device is described for pre-concentration and purification of analytes from biological samples (such as human serum, plasma, homogenized solid tissue, etc.) to be analyzed by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI MS) and methods of use thereof are provided.

A multiple function atmospheric pressure ion source interfaced to a mass spectrometer comprises multiple liquid inlet probes configured such that the sprays from two or more probes intersect in a mixing region. Gas phase sample ions or neutral species generated in the spray of one probe can react with reagent gas ions generated from one or more other probes by such ionization methods as Electrospray, photoionization, corona discharge and glow discharge ionization. Reagent ions may be optimally selected to promote such processes as Atmospheric Pressure Chemical Ionization of neutral sample molecules, or charge reduction or electron transfer dissociation of multiply charged sample ions. Selected neutral reagent species can also be introduced into the mixing region to promote charge reduction of multiply charged sample ions through ion-neutral reactions. Different operating modes can be performed alternately or simultaneously, and can be rapidly turned on and off under manual or software control.

The invention discloses a regression test case screening method based on partial multi-coverage, comprising the following steps of: firstly establishing a test requirement coverage matrix according to software system characteristics and a test case library; then aiming at a correction assembly, dividing a test requirement set into a concerned set and an independent set; selecting a regression test set by adopting an HATS (Host Access Transformation Service) algorithm; maintaining the test case library by adopting a multi-coverage category based on risk or operational profile definition test requirements; and screening the test case set by adopting an MHATS algorithm according to the given multi-coverage category. In the invention, redundant test cases can be reserved by adopting the multi-coverage category, therefore, the defect detection capacity of the test case library is maintained while the test case library is simplified; and in addition, the regression test case screening method is oriented to partial coverage while selecting the regression test set, simultaneously avoids from covering irrelative test requirements, and reduces the test execution and analysis costs while reducing the test case quantity.

The system for analyzing multiple samples includes a plurality of portable of sample supports each for accommodating a plurality of samples thereon, and an identification mechanism for identifying each sample location on each of the plurality of sample supports. The mass spectrometer is provided for analyzing each of the plurality of samples when positioned within a sample receiving chamber, and a laser source strikes each sample with a laser pulse to desorb and ionize sample molecules. The support transport mechanism providedprovides for automatically inputting and outputting each of the sample supports from the sample receiving chamber of the mass spectrometer. A vacuum lock chamber receives the sample supports and maintains at least one of the sample supports within a controlled environment while samples on another of the plurality of sample supports are being struck with laser pulses. The computer is provided for recording test data from the mass spectrometer and for controlling the operation of the system.

The invention discloses a preparation method and an application of an ionic liquid bonded silica adsorbent. The preparation method takes silica as a carrier, takes 3-aminopropyltriethoxysilane as a coupling agent to synthesize alkylsilane epoxy silica, and reacted with ionic liquid like 1,3-di-n-butyl imidazole hexafluoride phosphate to obtain the ionic liquid bonded silica adsorbent; the ionic liquid is fixed on the solid phase carrier silica through a chemical modification mode, characteristics of the ionic liquid can be maintained, and the stability during the usage process can be substantially enhanced; thereby the analysis cost can be reduced, and the precision of the determination result can be improved. The ionic liquid adsorbent by the chemical modification possesses good adsorption function to the hydrophobic substances such as a heavy metal ion chelate, and is suitable for separation and enrichment of trace hydrophobic compounds in a water phase sample in the fields of environment, food and the like.

The invention discloses a microfluidic concentration gradient droplet generating chip and a microfluidic concentration gradient droplet generating device. In the microfluidic concentration gradient droplet generating chip, a sample decentralizing channel, more than one reagent channel, more than one immiscible phase channel and a droplet reacting and detecting channel are processed on a chip base material processed by a quartz or organic solvent resistance material, a sampling probe integrated with the chip is processed at the inlet end of the sample decentralizing channel, the tail end of the sampling decentralizing channel is sequentially communicated with the outlet ends of the reagent channels and the outlet ends of the immiscible phase channel, and the outlet end of the sample decentralizing channel is communicated with the inlet end of the droplet reacting and detecting channel again. With the adoption of the microfluidic concentration gradient droplet generating device, a droplet array with the concentration gradient scope larger than three orders of magnitude can be formed by injecting a sample once, the consumption of the sample is less, and the generation of concentration gradient is quick. The microfluidic concentration gradient droplet generating device is especially suitable for high throughput screening research.

The invention discloses a near infrared detection method for the contents of the protein components in a peanut, which comprises the steps of: 1) building a calibration set sample spectrum; 2) preprocessing the calibration set sample spectrum; 3) extracting the characteristic information data of the calibration set sample spectrum; 4) building a correcting model; and 5) analyzing a sample to be detected. A near infrared spectrum of the sample to be detected is input into the correcting model after the near infrared spectrum of the sample to be detected is preprocessed and the characteristic information is extracted, and the contents of protein components in a sample nut can be obtained. The near infrared detection method for the contents of the protein components in the peanut provided by the invention has the advantages of being fast in analysis speed, high in analysis efficiency, low in analysis cost, and avoiding the environment pollution caused by any chemical reagent and any pollution etc., so that a reliable basis is provided for peanut quality analysis, peanut quality control and product quality.

The invention provides a detection method for veterinary drug residues. The detection method comprises the following steps of (1) treating a sample: pretreating the sample to be detected, adding an extracting agent, and performing treatment by multiple steps to obtain supernate, wherein filtrate obtained by filtration on the supernate is used for UPLC-MS / MS (ultra performance liquid chromatography-mass spectrum / mass spectrum) measurement; (2) measuring the veterinary drug residues: performing UPLC-MS / MS measurement on the obtained sample under a certain measurement condition. The detection disclosed by the invention has the characteristics of high sensitivity and high specificity and can conform to the detection on the veterinary drug residues.

The invention discloses an emission microscope chip failure analyzing method. The method comprises the following steps of: developing chip test stimulus by utilizing a hardware description language; simulating the chip test stimulus by software, burning the chip test stimulus into an FPGA (Field Programmable Gate Array) substrate after the simulation becomes normal; outputting a plurality of stimulus pattern outputs to a plurality of pins of a chip to be analyzed by the FPGA substrate to ensure that the circuit of the chip to be analyzed enters a failure stimulus mode; and capturing a light-spot through the emission microscope, and performing circuit analysis and failure analysis on the light-spot. The invention also discloses an emission microscope chip failure analyzing system. By adopting the emission microscope chip failure analyzing method and system, multi-channel complex test vector application can be realized, a defect of the emission microscope chip can be positioned, the cost for chip failure analysis can be reduced, and the analyzing efficiency can be improved.

The invention relates to a method for detecting physical and chemical indexes of reconstituted tobacco by a near infrared spectrum detection paper making method, which comprises the following steps of: carrying out the detection of the physical and chemical indexes on collected samples one by one by a standard method to obtain quantitative reference data; scanning and collecting spectrums of all the samples, carrying out spectrum preprocessing and eliminating the influence of noise and baseline drift; then corresponding spectrum data to the reference data of all physical and chemical indexes obtained by the standard method one to one and establishing a quantitative model by a partial least square method and mutual verification; carrying out accuracy and reproducibility inspection and then storing the quantitative model in a computer. When the physical and chemical component content of the reconstituted tobacco product samples to be detected needs to be detected, the near infrared spectrum data of the samples to be detected are scanned and collected, and the physical and chemical indexes of the samples to be detected can be analyzed by calling the quantitative model. The method has rapid and accurate detection and low analyzing cost without damaging the analyzing samples and pollution.

The invention discloses a method for measuring the content of mycotoxins in araliaceae plants by liquid chromatography-tandem mass spectrometry, comprising the following steps of: (1) preprocessing an araliaceae plant sample to be measured by a solid-phase extraction column; and (2) measuring the content of one or more mycotoxins in the preprocessed araliaceae plant sample through the liquid chromatography-tandem mass spectrometry. The measurement method is high in sensitivity, good in specificity and good in accuracy, and applied to measurement of multiple component residuals of the mycotoxins in the araliaceae plants.

The invention discloses a method for detecting the content of amino acid in peanuts. The method comprises the following steps of: 1) establishing a calibration set sample spectrum; 2) pretreating the calibration set sample spectrum; 3) extracting characteristic information data from the calibration set sample spectrum; 4) establishing a calibration model; and 5) analyzing a sample to be measured. The content of the amino acid in a peanut sample can be obtained through calculation by pretreating a near infrared spectrum of the sample to be measured and inputting extracted characteristic information into the calibration model. The invention has the advantages that: the method is high in analytical speed and analytical efficiency and low in analytical cost, chemical reagents are not used, environmental pollution is avoided, and the like; and a reliable basis can be provided for analyzing peanut quality and controlling peanut quality and product quality.

The invention relates to an automatic sampling distinguishing chemiluminescent multi-component immunological detection system and an analysis method of the same. An incubation system of the detection system consists of a test tube (1), a stir bar (2), magnetic beads (3) and a constant temperature magnetic stirring apparatus (11); a multi-channel sampling system consists of sampling channels (4), a magnet (12) and a glass tube (13); a solution conveying system consists of a peristaltic pump (14), a connecting pipe (10), a multifunctional syringe valve (15) and a multiposition valve (16); a signal acquisition system consists of a detection channel (9), a plane mirror (17) and a multiplier phototube (18); and the method is to fix various capture antibodies on the functionalized surfaces of the magnetic beads to perform specific reactions with an object to be detected and a tracing antibody so as to form a sandwiched complex, introduce chemiluminescent substrate liquid into the sandwiched complex after collecting and washing off excessive enzyme labeled antibodies by using the magnet, and inject the chemiluminescent substrate liquid into detection channels sequentially to perform detection. The method has the characteristics of simplicity, quickness, good reproducibility, high flexibility, low cost, and the like, and can be applied to the fields of clinical diagnosis, environmental monitoring, food security, and the like.

The invention relates to a convergence chromatography analytical method for residue of herbicides in tobacco and particularly relates to a method for detecting herbicides such as napropamide, alachlor, quizalofop-p-ethyl, diphenamid, metolachlor and clomazone in tobacco by virtue of a UPC<2>. The convergence chromatography analytical method comprises the following steps: firstly, carrying out extraction and primary decontamination on the herbicides contained in tobacco by virtue of a QuRChERS method; secondly, carrying out solid phase extraction purification on an extracting solution by virtue of a Florisil column so as to further reduce the interference of matrixes to a to-be-detected target; thirdly, detecting the herbicides by virtue of an ultra-performance convergence chromatographic instrument, and carrying out quantitative analysis by virtue of an external standard method. According to the convergence chromatography analytical method, all target substances can be analyzed within five minutes by virtue of the ultra-performance convergence chromatographic instrument, and the dosage of an organic solvent is extremely low in the analysis process; the convergence chromatography analytical method has the advantages of high efficiency, sensitivity and environment friendliness.

The invention discloses a method for analyzing a non-conductor material by utilizing a glow discharge mass spectrum, comprising the following concrete steps of: (a), processing the non-conductor material to be analyzed into a strip sample; (b), cleaning and stoving the strip sample; (c), placing metal indium into a silica crucible and heating to a molten state; (d), cladding the surface of the strip sample with a layer of metal indium membrane; (e), cleaning and stoving the strip sample again; and (f), carrying out direct current glow discharge mass spectrum analysis. By utilizing the analysis method provided by the invention, sample grinding and polishing are not needed, the pretreatment is simple and the pollution is avoided; the operation is simple and convenient, the usage amount of the metal indium is greatly decreased and the analysis cost is reduced; the discharge of the sample is similar to that of a conductor sample and is easily stabilized, the stability of a signal is good, the SNR(Signal to Noise Ratio) is ideal, the analysis time is shortened and the analysis efficiency is increased; the method is suitable for the analysis of massive or crystalline materials, the universality is strong and the application range is wide; and the sample has very good discharge stability and analysis repeatability.

The invention discloses a method for identifying human blood albumin products, comprising the following steps: (1) a citric acid buffer solution is prepared: 0.2mol / L of citric acid and 0.2mol / L of sodium citrate are taken and mixed according to the volume proportion of 12.3: 7.7, and each liter of buffer solution is added with 100mg of sodium azide, then the pH is adjusted to 4.2 by using 0.2mol / L of citric acid and 0.2mol / L of sodium citrate; (2) a BCG reagent is prepared: 105mg of bromocresol green is weighed and firstly dissolved in 2.5ml of 0.1mol / L NaOH (water-jacket heating for dissolution enhancement), then the mixture is added with 1000ml of 0.2mol / L citric acid buffer solution with the pH of 4.2, and 1 to 2ml of Tween minus 80 is added; (3) 2ml of BCG reagent is taken to be added with one drop of human serum albumin product which is ready to be detected, and an even shaking is carried out. If the color of the solution turns green from yellow, the product is an authentic product; otherwise, the product is a schlock. The identification method of the invention has low analysis cost, no need of special equipment, stronger specificity and high correct rate of the inspection results; the invention is applicable to the rapid inspection method of human blood albumin at the scene, and meets the needs of the basic pharmaceutical supervision and inspection.

The invention relates to a solid sampling-non-dispersion atomic fluorescence photometer collocating device and an analyzing method. The device comprises a solid sampler, an integrated interface, a sample pipe, a peristaltic pump, a tee joint, a gas-liquid separator, a detector and a computer. The method comprises the following steps of: volatilizing an analyzing element in a volatile halide way and absorbing by absorption liquid by adding an auxiliary heat release agent or an ashing auxiliary agent in the solid sampler. The absorption liquid is introduced into a chemical steam generating system for reacting with potassium borohydride (sodium); and the generated volatile hydride or atomic steam is carried into an instrument atomization area for being detected by atomic fluorescence. The invention directly adopts solid sampling, rapidly realizes the detection of toxic elements, such as arsenic, stibium, selenium, mercury, and the like in the samples, such as geology, environment, food, biology, and the like and has the advantages of higher analyzing speed, low analyzing cost, high detecting sensitivity, high device portability, environmental protection, and the like.

Disclosed is a bio lab-on-a-chip. The bio lab-on-a-chip is provided on a piezoelectric thin film on a substrate, and includes a sensing unit to sense a bio signal and a fluidic control unit which controls a transfer of a microfluid adjacent to the sensing unit. Provided is also a method of fabricating the bio lab-on-a-chip. The method includes the steps of forming a piezoelectric thin film, forming a sensing unit to sense a bio signal of a microfluid on the piezoelectric thin film, and forming a fluidic control unit located adjacent to the sensing unit.

The invention discloses a method for carrying out stress analysis on first-class nuclear reactors through using ANSYS software, belonging to the technical field of analysis and design methods for first-class nuclear reactors. The method comprises the following steps: firstly, after carrying out routine design according to a design specification, thus obtaining a design drawing; establishing a geometric model and a finite element model by using a simulation device in an ANSYS; carrying out analysis and calculation on all nuclear parts; extracting a stress analysis result; and carrying out strength assessment according to the specifications stipulated in subsections NB and NF of section III of ASME (American society of mechanical engineers), then obtaining a detailed design report. Compared with the prior art, the method disclosed by the invention summarizes and improves a method for carrying out analysis and design on first-class nuclear reactors through using ANSYS software; compared with an analytical method and a test method, by using the method disclosed by the invention, the design time is greatly shortened, the design expense is reduced, the analysis efficiency is greatly improved, and the analysis cost is reduced; and in the process of analysis, an entity is adopted for modeling a hexahedron unit, thereby improving the calculation accuracy.

The invention discloses a method for detecting three fat-soluble vitamins A, E and K1 in milk powder. The method comprises the following steps: weighing a sample, adding water to dissolve the sample and making a constant volume, placing part of the sample solution obtained after making the constant volume into a centrifugal tube, adding anhydrous ethanol, n-hexane and a saturated sodium chloride solution, uniformly mixing, centrifuging and layering, drying the n-hexane layer with nitrogen, dissolving with methanol to make a constant volume, detecting the dissolved solution and a standard work solution by a liquid chromatography-tandem mass spectrometer, respectively carrying out sample introduction to obtain a working curve with concentrations of vitamin A, vitamin A acetate, vitamin A plamitate, vitamin E, vitamin E acetate and vitamin K1 in the standard solution being the horizontal ordinate and with peak area of the six vitamins being the vertical coordinate; carrying out quantitative calculation on quantitative ion by the use of the working curve to obtain concentrations of the six vitamins in the sample solution to be measured; and obtaining contents of vitamin A, vitamin E and vitamin K1 by conversion. The method of the invention is simple, rapid and accurate, and has high sensitivity.

The invention relates to a continuous lossless whole rock natural gas generation simulation method. The method comprises the following steps of: crushing a hydrocarbon source rock sample into particles and loading into a sample tube of a thermal cracking device; connecting the sample tube and a pyrolyzing furnace of the thermal cracking device to a sample inlet of a chromatograph; regulating the pressure of carrier gas in the thermal cracking device; introducing gas into the thermal cracking device, fully adding liquid nitrogen into a liquid nitrogen cold trap, setting the heating temperature of the pyrolyzing furnace at the preset temperature, increasing the temperature of the thermal cracking device, and keeping the temperature for 50min after the temperature reaches the preset temperature to produce a gas product from the hydrocarbon source rock sample by thermal cracking; removing the liquid nitrogen cold trap and starting the gas chromatograph to analyze the gas product after the temperature of the thermal cracking device is reduced, and obtaining a chromatogram and a gas yield; changing the preset temperature and repeating the steps; and utilizing the gas yields at all the preset temperatures and the corresponding preset temperatures or the vitrinite reflectance of a coal rock sample to make a diagram within a same rectangular coordinate system, and forming a natural gas generation schematic diagram of the hydrocarbon source rock sample in different thermal evolution stages.

The invention discloses a numerical simulation method for a selective laser repair process. The method includes the steps of establishing a three-dimensional geometric model of repaired parts and an area to be repaired and performing finite element mesh division; determining repair route process parameters, wherein floor height number of repair paths, laser optimal power parameters and current pulse width parameters which are required for the repaired area are determined by the morphology of the repaired area; preprocessing parameters of powder materials of the repaired area and establishing heat source loading conditions; defining heat source parameters and using a life-death strain method to obtain a final temperature field; calculating a residual stress field; determining whether or notthe maximum equivalent stress of the repaired area meets the allowable stress of the powder materials according to a calculation result of stress distribution. The numerical simulation method can optimize the repair process parameters, increase the one-time repair rate of the parts, and reduce deformation generated due to thermal stress.

The embodiment of the invention discloses a quick detection system suitable for aviation oil wear particle information. The quick detection system comprises a laser-induced breakdown mechanism, a laser irradiation mechanism, a quartz pipeline, a pump, a hydrodynamic groove, a spectrum collection analysis mechanism and an imaging processing mechanism, wherein the quartz pipeline, the pump and the hydrodynamic groove can form a communicating loop, and a granular test sample can circularly flow in the loop; the laser-induced breakdown mechanism can output high energy laser pulse, and the high energy laser pulse is beam expanded and focused to the quartz pipeline to act on the granular test sample to generate plasma; a spectrum collection device can collect plasma spectrums, and the ingredients and the contents of all the metal elements in the granular test sample can be obtained through a spectrum analyzer; the laser irradiation mechanism can output continuous visible laser, the continuous visible laser is beam expanded to irradiate the granular test sample in the quartz pipeline, speckle images and metal particle shadow images can be formed on a receiving screen, and the speckle images and the metal particle shadow images are processed by a CCD imaging device to obtain the movement velocity information of metal particles and the actual sizes of the particles. The quick detectionsystem disclosed by the embodiment of the invention can quickly achieve change detection on ingredients and contents of the ingredients in large and small wear particles.

The invention provides a monolithic integrated micro-fluorescence analysis system and a manufacturing method thereof. The micro-fluorescence analysis system comprises a silicon substrate, a sample cell, a light emitting device, a silicon detector and an integrated circuit, wherein the sample cell is formed in the silicon substrate or bonded on the surface of the silicon substrate; the light emitting device is formed in a first selective area of the silicon substrate, and one light emitting side of the light emitting device is opposite to the first side of the sample cell; the silicon detector is formed in a second selective area of the silicon substrate, and one light detecting surface of the silicon detector is opposite to the second side of the sample cell; the integrated circuit is formed in the silicon substrate and is used for processing a detection signal, driving an excitation light source, analyzing the signal and controlling signal output. The fluorescence analysis system is processed on a same silicon-based platform by virtue of a micro electro mechanical system (MEMS) processing technology and a complementary metal oxide semiconductor (CMOS) process, thus being extremely small in size and extremely low in manufacturing cost and analysis cost, and a lab on chip (LOC) is truly achieved.

The invention belongs to the technical field of agriculture, and discloses a method for quantifying crop growth potential phenotypic parameters and analyzing yield correlation based on vision, which provides more ideas for agricultural analysis through continuous breakthroughs in image processing, wireless sensors, artificial intelligence and other technologies, and makes traditional manual agriculture and mechanized agriculture develop towards modern intelligence and refinement agriculture. As one of that important analytical subject in the agricultural field, the intelligent and refined agriculture has important significance in the field of plant virtual, yield prediction, garden design and the like. It is of great significance to analyze the characterization parameters and morphologicalcharacteristics in the process of crop growth. On the one hand, the method can predict the relevant parameters of crops, which can guide the allocation of breeding environment; virtual plant growth technology can simulate the plant growth state at any time, so as to reduce the analysis time and reduce the analysis cost.

Provided is a method for sorting a number of samples into an appropriate number of clusters according to their characteristics. Highly-correlated peaks are extracted from mass spectrum data obtained for the samples (S2). Using the extracted peaks, highly-correlated sample pairs are extracted (S3). While removing samples having low degrees of correlation, highly-correlated sample pairs are integrated to form core clusters (S4). Using singular peaks characterizing each core cluster, two or more core clusters are integrated to form clusters (S5-S7). These clusters include mixed clusters in which two or more clusters are mixed. Member determination formulae are created based on the singular peaks of each cluster (S8-S12). All samples, including those which have been excluded from the cluster determination process, are classified into clusters based on the member determination formulae (S14). The member determination formulae can also be used to assign a new sample to one of the cluster.

The invention belongs to the field of analysis and testing technology and specifically discloses a determination method for TOC of solid combustible matters. The method comprises the following steps: placing a solid combustible matter sample with certain mass into a combustor (an oxygen bomb) filled with an excess NaOH solution for complete combustion so as to thoroughly oxidize organic carbon into CO2; allowing the NaOH solution to completely absorb CO2 and converting CO2 into Na2CO3; adjusting a pH value to 8.32 by using a hydrochloric acid solution to convert Na2CO3 to NaHCO3; and measuring carbon content in NaHCO3 by using acid-base titration and calculating the TOC value of the solid combustible matter. The method provided by the invention has the characteristics of clear chemical principles, direct carbon measurement, reliable analysis, accurate analysis results, easy and convenient operation, low analysis cost and environmental protection and is applied to determination of TOC of solid combustible matters with complex composition and to related fields.