The invention relates to an integrated micro-fluidic
chip for screening
drug compatibility, which comprises a fluid flow passage layer, a microvalve
control channel layer and a glass substrate layer, wherein the fluid flow passage layer and the microvalve
control channel layer are thermally bonded through PDMS to form a fluid flow passage unit, the fluid flow passage unit and the glass substrate layer form a whole through
plasma bonding, the fluid flow passage unit comprises two functional areas, namely, a
medicine mixing generation area and an array
cell culture area. The invention further provides a preparation method and an application of the integrated micro-fluidic
chip for screening
drug compatibility; an SU-8 negative
photoresist technology, an AZ-XT50 positive
photoresist technology, a multi-layer PDMS
thermal bonding technology and a
plasma bonding technology are adopted, and the micro-fluidic
chip integrating functions of automatic
medicine mixing and generating,
cell culture, excitation, parallel analysis on compatibility results of different drugs, real-time observation and result detection is prepared, multiple experiment parameters can be obtained through once operation, and the integrated micro-fluidic chip is applied to the research on the high-flux
medicine compatibility relationship.