Method for detecting three fat-soluble vitamins A, E and K1 in milk powder

Abstract

The invention discloses a method for detecting three fat-soluble vitamins A, E and K1 in milk powder. The method comprises the following steps: weighing a sample, adding water to dissolve the sample and making a constant volume, placing part of the sample solution obtained after making the constant volume into a centrifugal tube, adding anhydrous ethanol, n-hexane and a saturated sodium chloride solution, uniformly mixing, centrifuging and layering, drying the n-hexane layer with nitrogen, dissolving with methanol to make a constant volume, detecting the dissolved solution and a standard work solution by a liquid chromatography-tandem mass spectrometer, respectively carrying out sample introduction to obtain a working curve with concentrations of vitamin A, vitamin A acetate, vitamin A plamitate, vitamin E, vitamin E acetate and vitamin K1 in the standard solution being the horizontal ordinate and with peak area of the six vitamins being the vertical coordinate; carrying out quantitative calculation on quantitative ion by the use of the working curve to obtain concentrations of the six vitamins in the sample solution to be measured; and obtaining contents of vitamin A, vitamin E and vitamin K1 by conversion. The method of the invention is simple, rapid and accurate, and has high sensitivity.

Description

technical field [0001] The invention relates to a method for detecting fat-soluble vitamins, in particular to three kinds of fat-soluble vitamins A, E and K in milk powder 1 detection method. Background technique [0002] Fat-soluble vitamins A, E, K 1 Often added to formula as a nutritional fortifier. Vitamin A, also known as retinol, has the main physiological functions of maintaining vision and bone health, participating in cell division and cell recognition, participating in growth, reproduction and maintaining the integrity of the immune system. Vitamin E, also known as tocopherol, has 8 types in nature: α-, β-, γ-, δ-tocopherol and α-, β-, γ-, δ-tocotrienol, among which α-tocopherol The strongest biological activity. Vitamin K 1 Also known as phytonadione, it controls blood coagulation and is an essential substance for the synthesis of coagulation proteins in the liver. These fat-soluble vitamins are unstable in the external environment and are easily oxidized an...

AI Technical Summary

Problems solved by technology

These fat-soluble vitamins are unstable in the external environment and are easily oxidized and destroyed by light, oxygen, etc.

At present, the determination method of fat-soluble vitamins in formula milk powder is usually: after the sample is processed by enzymolysis and saponification, it is extracted with a non-polar organic solvent (commonly used petroleum ether, n-hexane, ether, etc.) liquid-liquid extraction, and then reversed-phase chromatography Separation and detection by the method, the solvent consumption in the pretreatment process is large, the operation is cumbersome and the measurement results are unstable

[0003] Vitamin A and vitamin E are usually not directly added to formula milk powder, but vitamin A ester and vitamin E ester are added. Therefore, the sample needs to be saponified first, and then the vitamin to be tested is freed before testing. The method is complicated and cumbersome

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