The invention relates to a three-dimensional light stimulus
system comprising a
laser, a neutral color filter, a polaroid sheet, a
plane mirror, four lenses, a space light modulator, a computer, a dichroscope and an objective lens, the stimulus light transmitted by the
laser is incident on the space light modulator through the neutral color filter, the polaroid sheet, the
plane mirror and two lenses in order, the computer is connected with the space light modulator and controls the stimulus light generated incident by the space light modulator to generate a bit phase and to modulate into a stimulus light of
diffraction pattern, the stimulus light is irradiated by the two lenses, the dichroscope and the objective lens to the nearby area of the sample to form the
stimulus pattern of three-dimensional space distribution. The invention has advantages that, a plurality of stimulus points distributed in the three-dimensional space are formed simultaneously, and the position can be changed arbitrarily; the three-dimensional area of any shape can be stimulated. The invention can be applied to the research of the
stimulus response, can combine with the biomedical
observation system, can provide the means for the
biology and
medicine researches, and can be extensively used for the researches of chemical and physical and so on a plurality of academic subjects.