67 results about "Fungal toxin" patented technology

The invention discloses a method for detecting aflatoxin through an immunosensor. The aflatoxin (AFT) is a fungal toxin which has the high carcinogenicity, teratogenicity and toxicity, and accurate and rapid analysis on the aflatoxin is one of most effective means for reducing and avoiding harm of the aflatoxin. A prepared gold-polyaniline-graphene (Au-PANI-GN) composite nanomaterial is represented by adopting an ultraviolet spectrum and a transmission electron microscope and used for constructing the electrochemical immunosensor, the electrochemical immunosensor is represented by adopting a cyclic voltammetry method, an electrochemical AC impedance method and a square wave voltammetry method, and the prepared electrochemical immunosensor detects the aflatorxin B1. Experiment results show that the constructed electrochemical immunosensor has the good electrochemical activity on detection of the aflatoxin, and the concentrations of the aflatoxin and peak currents have the good linear relation, wherein the linear equation of the relation is y=-0.177x+4.28.

The invention discloses a method for measuring the content of mycotoxins in araliaceae plants by liquid chromatography-tandem mass spectrometry, comprising the following steps of: (1) preprocessing an araliaceae plant sample to be measured by a solid-phase extraction column; and (2) measuring the content of one or more mycotoxins in the preprocessed araliaceae plant sample through the liquid chromatography-tandem mass spectrometry. The measurement method is high in sensitivity, good in specificity and good in accuracy, and applied to measurement of multiple component residuals of the mycotoxins in the araliaceae plants.

A microorganism for the biological inactivation or detoxification of mycotoxins, in particular ochratoxins, which is selected from bacteria and / or yeasts, which cleaves the phenylalanine group of the mycotoxins, in particular ochratoxins, as well as a method for biologically inactivating or detoxifying mycotoxins, in particular ochratoxins, in food products and animal feeds by the aid of a microorganism, and the use of the microorganism(s).

Method for hyper-spectral imaging and analysis of a sample of matter, for identifying and characterizing an object of interest therein. Preparing test solution or suspension of the sample, including adding thereto a spectral marker specific to object of interest, such that if object of interest is in test solution or suspension, object of interest becomes a hyper-spectrally active target which is hyper spectrally detectable and identifiable; adding to test solution or suspension a background reducing chemical, for reducing background interfering effects caused by presence of objects of non-interest in test solution or suspension, thereby increasing hyper spectral detectability of hyper spectrally active target in test solution or suspension; generating and collecting hyper-spectral image data and information of test solution or suspension; and, processing and analyzing thereof. Exemplary objects of interest are biological agents—bacteria (Bacillus anthracis), viruses, fungi, toxins, or, chemical agents—nerve agents (sarin, tabun, soman), and chemical poisons.

The present invention relates to a method for detoxification of feed products contaminated by the mycotoxin zearalenone.

The invention discloses an LC (liquid chromatogram)-MS / MS(mass spectrometry / mass spectrometry) method for synchronously detecting 21 fungal toxins in grains. The method comprises the following steps:(1) pre-treatment of a sample; (2) purification; and (3) LC-MS / MS detection. The method is simple and convenient in operation and accurate in qualitative and quantitative analysis, and therefore, themethod can be taken as a reliable method for detecting various fungal toxins in the grains.

Relating to the field of removal of fungal toxins, the invention discloses a compound enzyme, an additive, application thereof and a method for removal of fungal toxins. Specifically, the invention relates to a compound enzyme, which contains amidase and esterase and can remove fungal toxins, especially fumonisins, ochratoxins and T2 toxin, additives containing the compound enzyme and applicationthereof in removal of fungal toxins, especially fumonisins, ochratoxins and T2 toxin, and a method for removal of fungal toxins. According to the technical scheme, combined use of the amidase and esterase can achieve simultaneous removal of ochratoxin A, fumonisins and T2 toxin, the removal efficiency is greatly improved than single use of one enzyme, and vomitoxins, aflatoxins and zearalenone toxin can be removed to certain degree.

The invention discloses engineering bacteria for efficiently degrading two fungal toxins and application. Coding gene of a high-efficiency degradation enzyme of two fungal toxins, i.e., deoxynivalenol (DON) and zearalenone (ZEN), is introduced into the engineering bacteria, so that the engineering bacteria with capacity of efficiently degrading the two toxins, i.e., the DON and the ZEN, is obtained. The engineering bacteria can be directly and effectively applied to diminishing the fungal toxins.

The invention discloses a cell electrochemical sensor for analyzing joint toxicity of fungaltoxin, belonging to the fields of analysis and detection of food quality. According to the cell electrochemical sensor, the sensitivity of the sensor is increased by virtue of electroplating gold nanoparticles, an in-vivo physiological living environment of cells is simulated by virtue of laminin, and a three-dimensional model is constructed by virtue of rat tail collagens so as to relatively truly reappear cell morphology; and the constructed cell electrochemical sensor can rapidly and sensitively respond to the influences caused by the fungaltoxin to human hepatoma carcinoma cells, equipment is convenient and rapid and low in production cost, and by combining with an electrochemical resistance value and a joint index method, the type and degree of a joint action of the fungaltoxin can be rapidly judged. The constructed portable cell electrochemical sensor is successfully applied to the toxicity analysis of the fungaltoxin in foods and has good application prospects in the analysis of the joint toxicity of the fungaltoxin.

The invention discloses a method for simultaneously detecting seven fungal toxins in original auxiliary materials of baijiu. According to the method, an improved QuEChERS method is adopted for extraction, further purification is not needed, high performance liquid chromatography is utilized for achieving separation of the fungal toxins, combined quadrupole-electrostatic field orbitrap mass spectrometry (Q-Orbitrab) is achieved, efficient parent ion selectivity and high-resolution accurate molecular weight (The mass number can be accurate to five digits after the decimal point.) are combined, accurate qualitativeness and quantification of the fungal toxins in baijiu and the original auxiliary materials can be achieved, and quantification is conducted through a matrix external standard method. The fungal toxins can be completely and effectively separated within 9 min, the linear relation of the fungal toxins within separate linear response ranges is good, the correlation coefficient R<2> is larger than 0.999, the lowest limit of quantification is lower than 1.0 microgram / L, the lowest detection limit is lower than 0.1 microgram / L, the recovery rate ranges from 70% to 120%, and the relative standard deviation (RSD) ranges from 0.5% to 4%. Compared with the national standard method, the method has the advantages of being rapid, simple, convenient, economical, efficient, safe and the like and can be suitable for separation and quantitative determination of various fungal toxins in baijiu and original auxiliary material samples.

An ozone / oxygen treatment system comprising an ozone generator for generating a predetermined ozone / oxygen mixture; and a treatment chamber connected to the ozone generator for receiving and applying the ozone / oxygen mixture to a predetermined portion of a patient's body, the treatment chamber having variable size and shape for enclosing said predetermined body portion and having a structure enabling the treatment chamber to enclose without touching the body portion. Also disclosed is a sensor disposed in the treatment chamber for sensing at least one of ozone concentration, temperature, humidity and bacterial gases. A control unit receives data from the sensor and automatically maintains the ozone concentration and / or heat or humidity at a predetermined range. Arrangements may be provided for directing the ozone to the body portion to be treated, and / or for directing the ozone to the interior and / or underneath a wound biofilm.

Mycotoxins prevailing in Northern climates have been until now proven difficult to deactivate in animal feed by binding, in contrast to mycotoxins prevailing in the Southern climates. Meanwhile, both types of toxins present a considerable risk factor for agricultural animal health and performance. A method of adsorbing and rendering harmless for animals an expanded range of both Northern and Southern mycotoxins found in contaminated feed is proposed. According to the invention, a combination of modified plant ligno-polysaccharide material and traditional mycotoxin binding components is added to the feed of agricultural and companion animals in an amount sufficient to abate the negative effects of both types of mycotoxins present.

The invention discloses a method for simultaneously detecting multiple fungal toxins in wheat, and concretely relates to a method for detecting vomitoxin (DON), aflatoxin B1 (AFB1) and zearalenone (ZEN) in wheat through immunoaffinity purification-high performance liquid chromatography. The method comprises the following steps: extracting a sample by using an acetonitrile-water solution having a certain ratio, purifying the obtained sample solution by a composite immunoaffinity column, simultaneously detecting the three fungal toxins through adopting the high performance liquid chromatography,preparing a standard working solution to qualitatively and quantitatively detect the three fungal toxins. Compared with the prior art, the method adopting the immunoaffinity chromatography to greatlyimprove the purifying degree of the sample and adopting the high performance liquid chromatography to realize simultaneous detection of multiple toxins has the advantages of operating time saving, high sensitivity, accuracy in quantification and good repeatability.

The invention discloses a method for measuring 8 kinds of mycotoxins of fructus citri medicae. According to the method, the 8 kinds of mycotoxins (ochratoxin A, zearalenone, fumonisin B1, sterigmatocystin, aflatoxin B1, aflatoxin B2, aflatoxin G1 and aflatoxin G2) are measured simultaneously in a system, a Thermo Hypersil GOLD chromatographic column is adopted for separation, and 0.1% formic acidwater-formic acid acetonitrile is used as a mobile phase; an electrospray ionization mode is adopted, and negative and positive ions scan simultaneously. A result shows that according to the method, the relations of the 8 kinds of mycotoxins in the linear range are good; the detection limit is at 0.16-5 ng / mL, the recovery rate at the low, medium and high adding levels is 79.0-112.8%, and an isotope internal standard method is adopted. According to the method, the matrix effect has little influence on the mycotoxin detection result, the accuracy is high, the precision degree is high, the specificity is high, and the method is simple, convenient and fast and can be used for multi-component measurement of mycotoxins in traditional Chinese medicine materials.

The invention relates to a safety evaluation method for analyzing a fungus strain producing no mycotoxin, based on a whole genome sequence. The method belongs to the fields of bio-fermentation technology and food safety. According to the invention, with a bioinformatics method, mycotoxin synthesis and metabolism pathways and key genes are analyzed, wherein PKS, NRPS and PKS-NRPS hybrid metabolic pathways, terpenoid compound metabolic pathways and other metabolic pathways are analyzed. Based on sequenced fungal whole genome data, whether the fungus has the latent mycotoxin producing capacity is analyzed, and the safety of the fungus in food industries in the aspects of fermented products and microbial strains is evaluated. As a recult, rhizopus chinensis CCTCCM201021 basically has no key gene or synthesis capacity for producing currently known mycotoxin, and a fermented product thereof is relatively safe. However, some strains of aspergillus species such as aspergillusniger have part of mycotoxin synthesis key gene clusters, and strain safety needs further analysis and determination. The method provided by the invention has important guiding and application values.

The invention discloses a ZEN (zearalenone) toxin degrading enzyme Oxa of acinetobacter as well as a coding gene and application thereof. An amino acid sequence of the ZEN toxin degrading enzyme Oxa of acinetobacter is as shown in SEQ ID NO.6. A nucleotide sequence of the coding gene A4-Oxa of the ZEN toxin degrading enzyme Oxa of acinetobacter is as shown in SEQ ID NO.5. The ZEN toxin degrading enzyme Oxa of acinetobacter disclosed by the invention has stronger ability of degradation on mycotoxin zearalenone; the Oxa enzyme for prokaryotic recombinant expression can degrade over 30% of ZEN toxins within 12 hours. According to the invention, a non-peroxidase coding sequence, i.e., an A4-Oxa gene of Acinetobacter sp. SMO4-degreaded zearalenone toxin is determined, so that a foundation is laid for researching an active site of the enzyme and site-specific mutagenesis Oxa enzyme by a specific site in future.

The invention relates to the field of microbes and especially relates to a method for degrading zearalenone through aspergillus niger. The method comprises A, spore fluid suspension preparation, B, inorganic salt medium optimization and C, zearalenone degradation. A pure culture obtained through the method can be used for degrading fungaltoxin zearalenone pollutants in various types of feed and has a degradation rate of 99%. The method has simple processes and is convenient for operation. The method provides an effective method for removing fungaltoxin zearalenone in animal feed.

The invention belongs to the technical field of food detection, and discloses a method for detecting seven typical mycotoxins in fruits and vegetables simultaneously. The method includes subjecting to-be-detected fruit and vegetable samples to ultrasonic extraction by formic acid-acetonitrile, performing centrifugation, drying supernatant, adding a formic acid aqueous solution before evenly mixing, performing extraction by an HLB (hydrophile-lipophile balance) solid-phase extraction column, and redissolving extract in a mixed solution of a ammonium acetate aqueous solution and acetonitrile toobtain to-be-tested liquid; preparing a mixed standard solution containing the seven typical mycotoxins, namely AOH, AME, TeA, TEN, DON, PAT and OTA, from the mixed solution of the ammonium acetate aqueous solution and the acetonitrile, acquiring a standard curve through UHPLC-MS / MS detection, detecting the to-be-detected liquid under same conditions, and calculating the contents of the seven typical mycotoxins in the to-be-tested liquid according to the standard curve. The method is applicable to simultaneous determination of four major types of the seven mycotoxins in the fruits and the vegetables, makes up for the defect of singularity of detection targets, and has the advantages of low detection limit and high detection accuracy.

The invention relates to a method for detecting 9 kinds of mycotoxins in a cassia seed medicinal material. The method comprises the following steps: a step 1: preparation of a standard substance stocksolution; a step 2: preparation of a mixed standard substance stock solution; a step 3: preparation of a standard working solution; a step 4: preparation of a tested object solution; a step 5: preparation of a substrate matching standard solution; a step 6: determination.

Disclosed are specific mutants of L3 and transgenic plants that produce them. The plants exhibit increased resistance to fungal toxins that target ribosomal L3 protein. Also disclosed are transgenic plants that co-produce L3 mutant and an RIP protein, and exhibit increased resistance to various fungal toxins and viruses, while reducing toxicity normally associated with production of the RIP. Uses of the L3 mutants in animals are further disclosed.

The invention provides a bacterial isolate defined by accession number 180507-1 filed with the International Depository Authority of Canada. The bacteria are capable of degrading trichothecene mycotoxins. Also provided are compositions comprising the bacteria and methods of preventing or treating food, foodstuffs, crops and harvested crops that are contaminated or susceptible to contamination with trichothecene mycotoxins. Kits are also provided.

The invention discloses a novel method for efficient determination of trace of deoxynivalenol based on surface enhanced Raman scattering. Silver nanoparticles are taken as a substrate of surface enhanced Raman scattering, and by determining the surface enhanced Raman scattering (SERS) spectrum of deoxynivalenol (DON) in grain, the method can be used for detecting whether DON in the grain exceeds a limit or not. According to the method disclosed by the invention, the DON detection limit on the surfaces of corn and kidney bean is 10-6M and the DON detection limit on the surface of oat is 10-4M, much lower than a specified limit standard at present. The method disclosed by the invention can be used for analyzing DON in the grain by virtue of the SERS technology for the first time and has the effects of being rapid, sensitive, accurate and convenient; the method has the advantages that test cost is reduced and on-site detection is realized. Compared with other traditional methods, the SERS technology provided by the invention can be better applied to the detection of various fungal toxins; and the method is a novel testing method having potential application value as well as development and application prospect.

The invention discloses bacillus velezensis Shui-6, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacillus velezensis Shui-6 is CGMCC No. 22256. The invention further discloses a production method of gamma-polyglutamic acid. The production method comprises the following steps: culturing the bacillus velezensis Shui-6; and harvesting gama-polyglutamic acid from the fermentation broth. The invention also discloses application of the bacillus velezensis Shui-6 in inhibition of fungal growth, degradation of fungaltoxin, production of gamma-polyglutamic acid or scientific research. The bacillus velezensis Shui-6 disclosed by the invention can be used for efficiently inhibiting the growth of fungi, efficiently degrading fungaltoxin and highly producing low-molecular-weight gamma-polyglutamic acid, and has a good application prospect in the aspects of developing microbial preparations for inhibiting the growth of fungaltoxin producing bacteria and microbial preparations for highly producing low-molecular-weight gamma-polyglutamic acid.

Fusarium graminearum is a plant pathogen, attacking a wide range of plant species including corn (ear and stalk rot), barley, and wheat (head blight). Fusarium epidemics result in millions of dollars of losses in crop revenues. Fusarium graminearum infection in the cereals reduces both grain yield and quality. Mycotoxins are produced by many fungal Fusarium species and thus the grain becomes contaminated with these mycotoxins, such as the trichothecenes. The major trichothecene produced by F. graminearum is deoxynivalenol (abbreviated as DON, also known as vomitoxin). Trichothecenes are potent protein synthesis inhibitors and are quite toxic to humans and livestock. A yeast gene has been identified which confers upon yeast tolerant of the trichothecene, trichodermin. A corresponding plant gene has been prepared, which has been used to transform plants. These transformed plants have an increased resistance to Fusarium infestation.

The invention discloses a method for inhibiting the growth of Alternaria alternata and the accumulation of mycotoxins in Alternaria alternata. The method is characterized in that a bacteriostatic agent used in the method is natural cinnamaldehyde essential oil which has a significant inhibitory effect on the Alternaria alternaria, with the minimum inhibitory concentration (MIC) being 0.2 MuL / mL. The mycotoxins are Alternariol (AOH) and Alternariol monomethyl ether (AME). After treatment with the cinnamaldehyde essential oil under MIC and 10 x MIC, toxin contents of the AOH and the AME in the Alternaria alternata decrease significantly. Therefore, the cinnamaldehyde essential oil, as a safe and pollution-free natural preservative, can not only control pathogenic bacterium Alternaria alternata after grapes are harvested, but also degrade the mycotoxins in the Alternaria alternata. The method has broad application prospects.

The invention provides a method for detecting multiple fungal toxins in an egg. The method comprises the following steps: step 1, sequentially adding water and an organic extracting agent into a raw and fresh egg sample which is evenly stirred, whirling and then carrying out mechanical oscillation for certain time; step 2, adding a certain amount of salt into the egg sample after the oscillation extraction, whirling, centrifuging to obtain a supernatant, and drying the supernatant; and step 3, redissolving the dried sample with a mobile phase applied to liquid chromatography tandem mass spectrometry, then filtering and carrying out qualitative or quantitative detection on the fungal toxins by virtue of a liquid chromatograph / mass spectrometer. According to the method, few pretreatment steps are carried out on the sample, so that the time consumption of a sample pretreatment phase in a whole food analysis experiment is greatly reduced, the loss of a target compound in an operating process is lowered, system errors are greatly reduced and the extraction efficiency of the target compound is improved; and all adopted reagents are very cheap and are less in usage amount, so that the experiment cost is lowered and the environmental pollution is reduced.