Novel method for efficient determination of trace of deoxynivalenol based on surface enhanced Raman scattering

Abstract

The invention discloses a novel method for efficient determination of trace of deoxynivalenol based on surface enhanced Raman scattering. Silver nanoparticles are taken as a substrate of surface enhanced Raman scattering, and by determining the surface enhanced Raman scattering (SERS) spectrum of deoxynivalenol (DON) in grain, the method can be used for detecting whether DON in the grain exceeds a limit or not. According to the method disclosed by the invention, the DON detection limit on the surfaces of corn and kidney bean is 10-6M and the DON detection limit on the surface of oat is 10-4M, much lower than a specified limit standard at present. The method disclosed by the invention can be used for analyzing DON in the grain by virtue of the SERS technology for the first time and has the effects of being rapid, sensitive, accurate and convenient; the method has the advantages that test cost is reduced and on-site detection is realized. Compared with other traditional methods, the SERS technology provided by the invention can be better applied to the detection of various fungal toxins; and the method is a novel testing method having potential application value as well as development and application prospect.

Description

Technical field [0001] The invention belongs to the field of instrumental analytical chemistry, and specifically relates to a novel and efficient method for determining DON in grains based on surface enhanced Raman scattering. More specifically, it relates to a surface-enhanced Raman scattering (SERS) spectrum that uses silver nanoparticles as a surface-enhanced Raman scattering substrate, and uses a portable Raman system to measure the surface-enhanced Raman scattering (SERS) spectra of DON in corn, kidney beans, oats and other grains. A new and efficient method for detecting trace amounts of DON in grains for the determination of vomitin in grains. Background technique [0002] Mycotoxins are secondary metabolites of fungi in grains, which are produced in the process of field, transportation, processing or storage. Every year, 25% of crops worldwide are contaminated by mycotoxins, which causes serious economic losses and safety risks. The scientific name of vomitin is deoxyni...

AI Technical Summary

Problems solved by technology

[0006] Raman spectroscopy is very sensitive to the symmetric vibration of covalent bonds in non-polar groups. It is insensitive to water and has few spectral overlapping bands, which provides more effective information for the detection of DON in grains. However, ordinary Raman The absorption intensity of the spectrum is not high, resulting in the detection sensitivity not meeting the requirements

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