229 results about "Fungal Toxins" patented technology

The invention relates to a method for detecting the content of mycotoxins in wheat. The mycotoxins in the wheat are detected by utilizing high performance liquid chromatography-tandem mass spectrometry, and the mycotoxins related to the detection are deoxynivalenol (DON), 3-acetyl (Ac)-DON, 15-Ac-DON, zearalenone (ZEN) and T-2 respectively. The detection method specifically comprises the following steps of: crushing wheat samples by using a crusher, and obtaining coarse extract by using an extracting solution; obtaining a purified toxin extracting solution by using an amino column; preparing mixed toxin standard liquid with different content; detecting each mycotoxin in the toxin extracting solution by adopting the high performance liquid chromatography-tandem mass spectrometry; and obtaining a regression equation of mycotoxin content relative to a peak area according to detection results of the mixed toxin standard liquid, and calculating the content of the DON, the 3-Ac-DON, the 15-Ac-DON, the ZEN and the T-2 in the wheat sample.

The invention relates to the biotechnology field, in particular to a fumonisin degrading enzyme, a coding gene, a recombinant vector, a cell, an additive and application thereof, and more specificallyrelates to the fumonisin degrading enzyme with a sequence shown as SEQ ID NO:2 or its mutant, the coding gene of the enzyme, the vector and cell containing the coding gene, the additive containing the enzyme and / or cell and / or the fermentation product thereof, application of the enzyme, the coding gene, the vector, the cell or the additive in degradation of fumonisins and / or other fungal toxins,as well as a method for degradation of fumonisin / or other fungal toxins. The enzyme provided by the invention has the advantages of: environmental friendliness, efficient and short time degradation offumonisins, and no production of harmful by-products. The enzyme can tolerate catalysis at high temperature up to 70DEG C, has low requirement for pH value and good stability, and also can degrade vomitoxin and T2 toxin to a certain degree.

The invention discloses expression equipment for expressing exogenous protein by secretion in trichoderma reesei cells. The expression equipment comprises the following elements from 5' to 3': (1) an exo-glucan cellobiose hydrolase II promoter of trichoderma reesei; (2) a secretively-expressed signal peptide; (3) a polyclonal locus sequence; and (4) an exo-glucan cellobiose hydrolase II terminator of the trichoderma reesei. Exogenous genes are inserted into the expression equipment, agrobacterium tumefaciens is converted by a T-deoxyribonucleic acid (DNA) binary vector and is jointed with thetrichoderma reesei to obtain trichoderma reesei genetic engineering bacteria which can express heterologous genes from animals, plants, fungi and the like efficiently by secretion, and a large amountof exogenous protein is obtained from the trichoderma reesei genetic engineering bacteria. The trichoderma reesei has an extensive culture condition, and is suitable for solid culture and liquid submerged fermentation; and mycotoxin and antibiotics cannot be generated under the zymogenic condition, and the generated extracellular protein is easy to separate and purify and low in cost.

The invention provides a bacterial isolate defined by accession number 040408-1 filed with the International Depository Authority of Canada. The bacteria are capable of detoxifying trichothecene mycotoxins. Also provided are compositions comprising the bacteria and methods of preventing or treating food or foodstuffs that are contaminated or susceptible to contamination with trichothecene mycotoxins. Kits are also provided.

The invention relates to immunochromatography technologies, and aims at providing a bigeminy qualitative fungaltoxin colloidal gold immunochromatography test strip and a preparation method. The test strip comprises a black strip-like polyvinyl chloride backboard taken as a base material, wherein the surface of the test strip is successively provided with a sample pad, a colloidal gold antibody marker fixing pad, a nitrocellulose membrane and an adsorption pad along the length direction of the backboard, and the adjacent pad or the membrane are in overlap joint with each other; and the nitrocellulose membrane is provided with two detection lines and one quality control line which have the same width with that of the backboard and are separated from one another, wherein the quality control line is positioned between the two detection lines. According to the test strip provided by the invention, the colloidal gold, the cost of which is relatively low, is adopted as an antibody marker, a competition method principle is adopted to quickly detect fungaltoxin, the nitrocellulose membrane wraps the two detection lines simultaneously, the color depth can be compared by naked eyes, and qualitative detection can be carried out quickly on two kinds of fungaltoxin simultaneously. The chromatography test strip prepared has excellent sensitivity and stability, and is easy, convenient and fast to operate and low in cost, thereby being particularly suitable for rapid diagnosis on site.

The present invention relates to an immunochromatography time resolution fluorescence kit for synchronization detection of mixed pollution of fumonisin B1 and other four fungaltoxin and application thereof. The immunochromatography time resolution fluorescence kit comprises an immunochromatography time resolution fluorescence test paper strip and a sample reaction bottle containing europium-labeled freeze-dried products of all monoclonal antibodies; the immunochromatography time resolution fluorescence test paper strip includes a PVC substrate, a water absorbent pad, a testing pad and a sample pad which are respectively pasted on one side of the PVC substrate from top to bottom, the testing pad is provided with a nitrocellulose membrane as a base pad, the nitrocellulose membrane is provided with a horizontal quality control line and four detection lines from top to bottom, the four detection lines are respectively coated with bovine serum albumin conjugates of all the fungaltoxin, and a fumonisin B1 monoclonal antibody is secreted by hybridoma cell line Fm7A11 with preservation number of CCTCCNO.C201636. The immunochromatography time resolution fluorescence kit can be used for synchronization detection of content of aflatoxin B1, ochratoxin A, zearalenone and the fumonisin B1, and has the characteristics of simple operation, rapidness and high sensitivity.

The invention provides an intelligent nitrogen-enriched ozone atmosphere-controlling quality-guaranteeing freshness-preserving method and an intelligent nitrogen-enriched ozone atmosphere-controlling quality-guaranteeing freshness-preserving device for foodstuff storage and transportation process. The device consists of a nitrogen and oxygen separation system, an ozone generation system, a temperature and humidity and gas concentration test control system, a direct circulation air supply system, a 360-degree uniform flow atmosphere control air supply system, and an intelligent control system. According to the method and the device, stored foodstuff is subjected to circulation atmosphere control, quality adjustment and freshness preservation or quality preservation drying treatment by utilizing the characteristics of nitrogen and ozone and taking air as a carrier, gas composition and temperature and humidity in a barn are changed to damage the growing environment of pests and molds, and the physiological respiration of the foodstuff is adjusted, so that mold resistance and sterilization, moth resistance and insect disinfestation, quality adjustment and freshness preservation, quality preservation drying and safe detoxication can be realized, and aims of economy and practicability and environment-friendly foodstuff storage are fulfilled. Compared with the conventional foodstuff preservation technology, the method and the device not only have the functions of mold and moth resistance and quality keeping, but also have the functions of quality preservation drying and quality adjustment and freshness preservation. By the method and the device, the functions of keeping quality and preserving freshness of the stored foodstuff with over high water content can be realized, the quality of the stored foodstuff can be improved and water content decrement loss is reduced. The device is environment-friendly, and mycotoxins and organic phosphorus pesticide residues in the foodstuff can be degraded, and the device and the method do not cause secondary pollution to the foodstuff and storage and transportation environment.

The invention discloses a detection method of fungaltoxin DON (deoxynivalenol) and a detection kit. The method comprises steps as follows: DONApt and a single-stranded signal probe ssDNA are hybridized, and a DONApt-ssDNA (single-stranded deoxyribonucleic acid) hybrid chain is formed; a to-be-detected sample is added to the DONApt-ssDNA hybrid chain, and when the to-be-detected sample has DON, DONApt-ssDNA reacts with DON to generate DONApt-DON and release ssDNA; remaining DONApt-ssDNA hybrid chain in the system forms double-stranded DNA through DNA amplification; double-stranded DNA is hydrolyzed into mononucleotide under catalysis of exonuclease and is removed, and ssDNA in the system is reserved; silver ions and a reducing agent are added to the system, and silver ions are reduced to generate near infrared fluorescence silver nano-clusters under induction of ssDNA; finally, the content of DON in the to-be-detected sample is calculated according to the relation between the near infrared fluorescence intensity and the quantity of DON.