64 results about "Trichothecene" patented technology

Sesquiterpene epoxide compounds (trichothecenes) and methods for administering such compounds to achieve apoptotic ablation of internal organs or internal non-malignant cell populations are disclosed.

Sesquiterpene epoxide compounds (trichothecenes) and methods for administering such compounds to chemexfoliate selected epidermal cell populations are disclosed.

The invention discloses a myrothecium roridum A553 trichothecene-resistant self-protection gene mfs1 and an application thereof. The nucleotide sequence of the myrothecium roridum A553 trichothecene-resistant self-protection gene mfs1 is as shown in SEQ ID NO. 1. According to the invention, a transcriptome sequencing result is used to predict a gene sequence, a cDNA library is used as a template,and amplification is carried out to obtain the myrothecium roridum A553 trichothecene-resistant self-protection gene mfs1. The myrothecium roridum A553 trichothecene-resistant self-protection gene mfs1 can efficiently assist saccharomyces cerevisiae in resisting trichothecene toxins, and lay a molecular biological foundation for improving the trichothecene-resistant capacity of saccharomyces cerevisiae in the later period, improving the heterologous expression level of trichothecene toxins and obtaining novel trichothecene toxins.

Microorganism of the genus Eubacterium, and its obtainment and use, which is suitable in pure culture, DSM 11798, and / or mixed culture with the strain Enterococcus casseliflavus, DSM 11799, or in mixed culture with other anaerobic microorganisms for the detoxification of trichothecenes.A feedstuff additive for the inactivation of trichothecenes in feedstuffs or in the digestive tract of animals contains a pure and / or mixed culture of the microorganism (DSM 11798 or DSM 11799) or a mixed culture with other anaerobic microorganisms in an amount from 0.2 to 3 kg, in particular 0.5 to 2.5 kg, per 1000 kg of feedstuff. The feedstuff additive containing DSM 11798 achieves probiotic effect on an animal and maintains or improves fertility performance of an animal subject to fusariotoxin-contaminated feed.

The invention provides a bacterial isolate defined by accession number 040408-1 filed with the International Depository Authority of Canada. The bacteria are capable of detoxifying trichothecene mycotoxins. Also provided are compositions comprising the bacteria and methods of preventing or treating food or foodstuffs that are contaminated or susceptible to contamination with trichothecene mycotoxins. Kits are also provided.

The invention discloses a myrothecium roridum A553 trichothecene synthase gene Tri5 promoter and application thereof. The nucleotide sequence of a promoter is shown in SEQ ID NO.1. According to the promoter, through a chromosome walking technology, the upstream promoter sequence of the Tri5 gene is obtained, the core region of the promoter is predicted, and the myrothecium roridum A553 trichothecene synthase gene Tri5 promoter Tri5P is obtained. By means of the promoter, expression of a hygromycin resistance gene can be efficiently started, the starting efficiency is close to that of a constitutive promoter TEF1, and a molecular biology basis is laid for increasing the yield of trichothecene toxin and obtaining more novel trichothecene toxin with high activities through transcriptional regulation and heterologous expression in the later period.

The present document is directed to detoxification of trichothecenes using a method involving trichothecene epoxide ring opening. In particular, the present document discloses the detoxification of a trichothecene by reaction with a thiol containing compound under conditions where the pH is equal to or higher than one pH unit less than the pKa of a thiol group of the thiol containing compound.

The invention discloses a special test box for the enzyme-linked immunosorbent assay of class-B trichothecenes, which has the advantages of fast, sensitive and accurate detection, quantification, simplicity in operation, low requirement on the sample purity and strong specificity and is particularly suitable for mass samples. Therefore, the invention also provides a preparation and detection method of the special test box. The special test box is characterized by comprising a concentrated washing solution, a concentrated complex solution, a color developing solution A, a color developing solution B, a stop solution, a coating board coating the solid-phase antigen against class-B trichothecenes, a standard product of class-B trichothecenes and an antibody freeze-dried product of class-B trichothecenes. After the detection, an absorbance value is detected at 450nm, and the content of class-B trichothecenes in the sample is calculated from a standard curve.

The invention discloses a kit for synchronously detecting six fungal toxins. The six toxins are aflatoxin, ochratoxin, patulin, trichothecene mycotoxin, fumonisins and zearalenone; and based on a multiple PCR detection method, the kit comprises a first primer pair, a second primer pair, a third primer pair, a fourth primer pair, a fifth primer pair, a sixth primer pair and an internal standard primer pair which can perform PCR reaction in the same reaction tube. The detection system directly targets a toxin synthesis essential gene and has high specificity; an internal reference gene is amplified synchronously, positive, negative and blank control are set simultaneously, and false negative and false positive are effectively avoided; and multiplication of PCR index guarantees the sensitivity of the method.

One embodiment relates to a mycotoxin adsorbent highly specific for A and B trichothecenes, and especially for vomitoxin (or deoxinivalenol) and T-2 toxin; as well as a process for the preparation of the mycotoxin adsorbent. The adsorbent may be obtained by modifying the surface of an aluminum silicate using an organic functionalized-chain compound which provides it with high polarity properties. The adsorbent may be used in the preparation of balanced animal feed that avoids the toxic effects of the trichothecenes present in the feed. The combination of the adsorbent with aluminum silicates or organic aluminum silicates may be used for the preparation of balanced animal feeds that avoid the toxic effects of mycotoxins.

The invention discloses a set of primer for detecting trichothecene group B-class toxin of fusaria through PCR (polymerase chain reaction). The primer comprises a share sense primer F shown in SEQID NO.1, 3-acetyl deoxygenation nivalenol reverse primer R1 shown in SEQID NO.2, 15-acetyl deoxygenation nivalenol reverse primer R2 shown in SEQID NO.3 and nivalenol reverse primer R3 shown in SEQID NO.4; the set of the primers can be applied to the PCR for directly detecting 3-acetyl deoxygenation nivalenol, 15-acetyl deoxygenation nivalenol and nivalenol. The primer is low in cost, simple in operation, short in consumption time, accurate in testing result and easy for large-scale popularization and application.

The invention discloses a promoter of a trichothecene synthase gene Tri12 of Leucosphaeria dothidea A553 and the application of the promoter. The nucleotide sequence of the promoter is as shown in SEQID NO. 1. According to the invention, an upstream promoter sequence of the Tri12 gene is obtained through a chromosome walking technology, and a promoter core region is predicted so as to obtain thepromoter Tri12P of the trichothecene synthase gene Tri12 of the Leuconostoc dunalianum A553. The promoter can efficiently start expression of the hygromycin resistance gene hph, and the starting efficiency is similar to the starting efficiency of a constitutive promoter TEF1, so a molecular biological foundation is laid for increasing the yield of trichothecene toxin and obtaining more high-activity novel trichothecene toxin through transcription regulation and heterologous expression in a later period.

The invention provides a bacterial isolate defined by accession number 180507-1 filed with the International Depository Authority of Canada. The bacteria are capable of degrading trichothecene mycotoxins. Also provided are compositions comprising the bacteria and methods of preventing or treating food, foodstuffs, crops and harvested crops that are contaminated or susceptible to contamination with trichothecene mycotoxins. Kits are also provided.

The invention discloses a fusarium head blight virus toxin degrading gene and expression thereof. The fusarium head blight virus toxin degrading gene has a nucleotide sequence represented by SEQ ID No.1 and an amino acid sequence represented by SEQ ID No.2. The invention also discloses the expression of the gene, designs two pairs of primers, constructs a recombinant plasmid Pmd19-T / Tri101 and a recombinant prokaryotic expression vector pGEX-4T2 / Tri101. The pGEX-4T2 / Tri101 carries a glutathione S-transferase (GST) tag and can be expressed to produce a GST-Tri101 fusion protein. In the invention, a Tri101 gene for coding a trichothecene 3-O-acetyl transferase is expressed in Escherichia coli with high efficiency, the nucleotide sequence and amino acid sequence of the gene are analyzed in details, and a theoretical basis is laid for further study on the biological functions of the gene and the protein thereof and plant genetic engineering.

Sesquiterpene epoxide compounds (trichothecenes) and methods for administering such compounds to inhibit proliferation of psoriatic cell populations in the epidermis are disclosed.

Fusarium graminearum is a plant pathogen, attacking a wide range of plant species including corn (ear and stalk rot), barley, and wheat (head blight). Fusarium epidemics result in millions of dollars of losses in crop revenues. Fusarium graminearum infection in the cereals reduces both grain yield and quality. Mycotoxins are produced by many fungal Fusarium species and thus the grain becomes contaminated with these mycotoxins, such as the trichothecenes. The major trichothecene produced by F. graminearum is deoxynivalenol (abbreviated as DON, also known as vomitoxin). Trichothecenes are potent protein synthesis inhibitors and are quite toxic to humans and livestock. A yeast gene has been identified which confers upon yeast tolerant of the trichothecene, trichodermin. A corresponding plant gene has been prepared, which has been used to transform plants. These transformed plants have an increased resistance to Fusarium infestation.

The invention relates to a type-A trichothecene polyclonal antibody as well as a preparation method and application thereof. The method comprises the following steps: performing structural modification on T-2 toxin serving as a precursor to prepare a hapten 3-Ac-NEOS with immunoreactivity to an A-type trichothecene mother nucleus structure, coupling the hapten with macromolecular proteins BSA and OVA to convert a micromolecular hapten only having immunoreactivity into a complete antigen with immunogenicity, and immunizing animals. Prepared antiserum is relatively high in titer and the requirement of immunological detection on the antibody is met. An immunological detection technology established on the basis of the antibody is easy and convenient to operate, good in specificity and high in sensitivity, and can be widely applied to detection of type-A trichothecene (including hidden type-A trichothecene) in foods, feeds and animal bodies. For example, an ELISA kit prepared by adopting the antibody can be used for detecting hidden T-2 toxins in prawn bodies.

The invention discloses a biological detoxifying agent of trichothecene toxins and an application thereof for detoxifying in forage. The detoxifying agent is the mixture of at least three of ammonium nitrate, aluminosilicate, ammonium carbonate and yeast cell wall (according to any proportion) and NJA-1 bacterium, wherein the mass content of the NJA-1 bacterium is 40%-80%. The biological detoxifying agent of the trichothecene toxins is a harmless product which is obtained by biologically transferring or transferring the mycotoxins by the enzyme or metabolizing, can not lose the nutrition components, can not change the palatability, and is remarkable in detoxifying effect compared with the single NJA-1 bacterium.

The invention discloses a gene product detection method of degradative graminearum toxin. A rabbit anti-GST(glutathione transferase)-Tri101 antibody acting as a primary antibody, and a goat anti-rabbit IgG-HRP (horse radish peroxidase) acting as a secondary antibody and an object to be tested containing the Tri101 protein carry out Western blot analysis. The trichothecene 3-O-acetyl invertase coded by Tri101 gene in the invention is efficiently expressed in colibacillus; a purified protein immune New Zealand white rabbit obtains an antibody with relatively good specificity, and can carry out immunoblotting reaction with Tri101 protein secreted and expressed in BL21 (DE3); and a new detection method is provided for trangenosis of Tri101 gene in wheat and corns; the detection cost is reduced; and a solid basis for further researching the biological functions of Tri101 protein is provided.

A alcohol dehydrogenase of sequence ID no. 1 containing metal ions and a quinone cofactor, or in addition, a functional variant exhibiting a sequence identity of at least 80%, preferably at least 86%, especially preferred at least 89% and at least one redox cofactor for the transformation of at least one trichothecene exhibiting a hydroxyl group on the C-3 atom, as well as a method for the enzymatic transformation of trichothecenes and a trichothecene-transforming additive.

Microorganism of the genus Eubacterium, and its obtainment and use, which is suitable in pure culture, DSM 11798, and / or mixed culture with the strain Enterococcus casseliflavus, DSM 11799, or in mixed culture with other anaerobic microorganisms for the detoxification of trichothecenes.A feedstuff additive for the inactivation of trichothecenes in feedstuffs or in the digestive tract of animals contains a pure and / or mixed culture of the microorganism (DSM 11798 or DSM 11799) or a mixed culture with other anaerobic microorganisms in an amount from 0.2 to 3 kg, in particular 0.5 to 2.5 kg, per 1000 kg of feedstuff.

The invention belongs to the technical field of plant inspection and quarantine, and specifically relates to a molecular detection method of type-B toxins of fusarium and application thereof. The molecular detection method of the invention is characterized by comprising the steps of designing a pair of multiplex PCR primers through Tril 3 gene information aiming at the trichothecene type-B toxins, separating the fusarium graminearum for generating 3-AcDON, 15-AcDON and NIV toxins to obtain the specific segments of 334bp, 279bp and 497bp, using the PCR reaction, detecting the product by the agarose gel electrophoresis, and directly detecting the type of the toxins generated by the fusarium graminearum according to the size of the segment of the product. The invention is applicable for the detection of type-B toxins of fusarium in the grain, feed and food safety.

The invention discloses a trichothecene compound as well as a preparation method and application thereof. The trichothecene compound is characterized in that the structural formula of the trichothecene compound is shown as I. The preparation method comprises the following steps: preparing a leavening of a trichothecene compound from fusarium of which the collection number is CGMCC No. 14121 by microorganism fermenting and culturing, and performing filtration through gauze in order to separate a mycelium from a fermentation liquid; then, adding the mycelium into methyl alcohol for performing ultrasonic smashing, performing isovolumetric extraction with ethyl acetate for 3 times, and evaporating an extractant with a rotary evaporator to dryness to obtain a thallus extract; performing extraction on the fermentation liquid with the isovolumetric ethyl acetate for 3 times, combining and collecting an ethyl acetate phase, evaporating the ethyl acetate phase with the rotary evaporator to dryness to obtain a bacteria solution extract, and combining the thallus extract and the bacteria solution extract to obtain a main rough extract; lastly, dissolving the main rough extract with acetonitrile, and performing separation and purification with a semi-prepared reverse phase efficient liquid chromatograph. The trichothecene compound has the advantage of capability of restraining aquatic pathogenic bacteria of vibrio harveyi.

The invention provides a feed trichothecene mycotoxin biodegradation agent. Trichothecene mycotoxin comprises but is not limited to nivalenol, verrucarine and deoxynivalenol. By adopting the preparation method provided by the invention, a Devosia sp. ANSB714 for safely and efficiently degrading feed trichothecene mycotoxin is separated and screened out from a natural resource for the first time, and biological characteristics and toxin degradation characteristics of the bacterium are studied. The invention also provides a culture method of the bacterium, a method for preparing a biodegradation agent by using the bacterium, and a method for applying the biodegradation agent to degradation of the trichothecene mycotoxin in a feed. After the ANSB714 reacts with a moldy feed for 24 hours, the degradation rate of the trichothecene mycotoxin in the feed can reach 98%. The Devosia sp. ANSB714 in the invention has high activity, strong specificity and mild effect during degradation of the trichothecene mycotoxin, cannot damage nutritional ingredients in the feed, and improves the utilization ratio of the feed at the same time, so that the safety in production of animal husbandry is ensured and the economic benefit is improved.

The invention discloses a polypeptide having deepoxidation catalytic activity, and encoding nucleic acid and application thereof. The polypeptide provided by the invention can catalyze a reaction of trichothecene mycotoxins and glutathione under mild conditions to remove epoxy groups and generate non-toxic and harmless glutathione derivatives, so that detoxification and detoxification of trichothecene mycotoxins are realized. The polypeptide has wide applications in the fields of agriculture, food, feed, medicine and the like.

The invention discloses a detoxifying pharmaceutical composition and use thereof. The detoxifying pharmaceutical composition contains epoxy-free active polypeptides and optional pharmaceutically-acceptable carriers, wherein the active polypeptides are capable of enabling trichothecenes and glutathione to generate catalytic reaction to generate a glutathionylation derivative, so that epoxy groups generating toxin toxicity are removed, and a detoxification effect is achieved.

The invention discloses a loop-mediated isothermal amplification (LAMP) primer combination, an LAMP kit and an LAMP method for detecting trichothecenes. The LAMP primer combination consists of a forward outer primer F3, a reverse outer primer B3, a forward inner primer FIP, a reverse inner primer BIP, a forward loop primer LB and a reverse loop primer RB. The detection method disclosed by the invention can be used for simultaneously detecting all trichothecenes, and the detection method is strong in specificity, high in accuracy, simple and convenient to operate, good in practicability and capable of achieving, isothermal amplification; and the detection method is applicable to the high-sensitivity rapid detection of the trichothecenes and is easy for large-scale popularization.

The invention provides a bacterial isolate defined by accession number 040408-1 filed with the International Depository Authority of Canada. The bacteria are capable of detoxifying trichothecene mycotoxins. Also provided are compositions including the bacteria and methods of preventing or treating food or foodstuffs that are contaminated or susceptible to contamination with trichothecene mycotoxins. Kits are also provided.