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Detoxifying pharmaceutical composition and use thereof

A kind of composition and medicine technology, applied in the field of medicine

Active Publication Date: 2020-07-31
山东农之安生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, no definite genes or proteins have been reported to catalyze the detoxification of trichothecenes by deepoxylation

Method used

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  • Detoxifying pharmaceutical composition and use thereof
  • Detoxifying pharmaceutical composition and use thereof
  • Detoxifying pharmaceutical composition and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0059] 1. The active polypeptide FTCD catalyzes the removal of epoxy groups from trichothecenes

[0060] 1. Experimental method:

[0061] 1.1 In vitro enzymatic reaction:

[0062] 0.05mol / L phosphate buffer solution (PBS): 0.05mol / L Na 2 HPO 4 Mix with 0.05mol / L KH2PO4 at a ratio of 3:2, pH=7.0; 0.1mol / L glutathione (GSH): weigh 3.073g of glutathione, add distilled water to 100ml; 0.5mg / ml Trichothecenes (DON, 3DON, 15ADON, FUS-X, NIV, T2, H-T2, DAS): 1 mg of trichothecenes, add distilled water to 2 ml, filter to sterilize.

[0063] Dissolve DON, 3-DON, 15-ADON, NIV, DAS, HT-2, T-2 toxins (1 mg) in freshly prepared GSH (30.7 mg, 100 μmol) in PBS buffer, and add active peptides, Insulate in a 20°C water bath for 24h.

[0064] 1.2 LC-HRMS ( / MS) analysis

[0065] After the in vitro reaction solution was filtered through a 0.22 μm filter membrane, it was transferred to an injection vial for LC-HRMS detection. Using Thermo Scientific TM Q Exactive TM Combined quadrupole Or...

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Abstract

The invention discloses a detoxifying pharmaceutical composition and use thereof. The detoxifying pharmaceutical composition contains epoxy-free active polypeptides and optional pharmaceutically-acceptable carriers, wherein the active polypeptides are capable of enabling trichothecenes and glutathione to generate catalytic reaction to generate a glutathionylation derivative, so that epoxy groups generating toxin toxicity are removed, and a detoxification effect is achieved.

Description

technical field [0001] The present invention relates to the field of medicine, in particular to an antidote pharmaceutical composition and its application. Background technique [0002] Trichothecenes are toxic metabolites with similar biological activity and chemical structure mainly produced by Fusarium, Cephalosporium, Myrotheca, Staphylocera, Trichoderma and some other molds, and they are widely found in nature. Trichothecenes mainly exist in food, feed and their processed products contaminated by the above bacteria, such as barley, wheat, oats, corn and other grains. [0003] Consumption of food or feed contaminated by this toxoid can lead to loss of appetite, gastrointestinal inflammation, or bleeding, vomiting, diarrhea, necrotizing dermatitis, movement disorders, poor blood coagulation, anemia and decreased white blood cell count, decreased immune function and abortion, etc. , a serious threat to human and animal health. Excessive intake can easily lead to serious ...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61P39/02
CPCA61K38/168A61P39/02
Inventor 王宏伟其他发明人请求不公开姓名
Owner 山东农之安生物科技有限公司
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