49 results about "Trichodermin" patented technology

The invention discloses a method for manufacturing an inorganic nano-silver mildew-proof antibacterial healthy decorative veneer. The method comprises the following steps: (1) soaking a decorative veneer in aqueous solution of nano-silver, and drying the decorative veneer; (2) coating adhesive containing inorganic nano-silver on the surface of a base material; and (3) applying the decorative veneer on the adhesive on the surface of the base material, and performing compression molding on the veneer. The decorative veneer manufactured according to the method can resist invasion of mildew caused by dampness of the base material to the surface of the decorative veneer, ensures that sheet materials can achieve the effect of mildew proofing and bacteria resistance and has environment-friendly, non-toxic, high-efficiency, stable, long-acting mildew-proof antibacterial effect, has mildew-proof and antibacterial grade reaching the highest 0 level of JIS Z2911 standard, and can effectively improve the domestic living environment for people. The veneer can be used in the fields of building materials, furniture, indoor furnishing and decorating, vehicles and ships and the like. The healthy sheet materials decorated indoors have strong killing effect on epidemical bacillus, trichodermin, stain fungi and the like.

A process for preparing the biologic agricultural chemical from trichoderma includes such steps as reproductive culture of trichoderma, fermenting in liquid, inoculating it in the culture medium prepared from cane dregs, wheat bran and rice husk for solid fermenting, culturing at 25-28 deg.C under 60-80% of relative humidity for 4-5 days, culturing at low temp (lower than 12 deg.C) for 2-3 days, drying at 40-50 deg.C for 12-24 hr, adding promoter prepared from diatomite, wheat bran and sulfur, mixing, pulverizing and sieving.

The preparation method of organic ecological soilless culture medium belongs to the field of agricultural bio technology. It is made up by using 100 portions of straw powder, 50-60 portions of cumulose fermented livestock and fowl dried excrements and 3-5 portions of compound bacterial preparation formed from azotogen and trichoderma preparation through the processes of uniformly mixing and stirring. The preparation of azotogen adopts cellulose Nocardia HD-86 with functions of decomposing cellulose and fixing nitrogen as strain and adds the corn stover, bran and water, and utilizes the aseptic fermentation process to obtain the azotogen, and the preparation of trichoderma preparation uses pseudo-Kan's trichodermin S28 as strain, and uses cob, bran, ammonium sulfate as culture medium through the processes of pulverizing, mixing, moistening, inoculating S28 and fermentation so as to obtain the trichoderma preparation. Said invention is favorable for industrial production of vegetables.

The invention discloses a trichodermin derivative, and a structural general formula of the trichodermin derivative is shown as the right. The trichodermin derivative is used for killing plant pathogenic fungi such as Botrytis cinerea, Pythium ultinum Trow, fusarium, leafmold, Rhizoctonia solani, Fusarium oxysporum, Magnaporthe grisea and so on.

The invention provides a method for producing biologic protein feed by using solid-state fermentation fiber dreg. The method comprises the steps: activating, inoculating, cultivating and fermenting lignin trichodermin strain, candida utilis and saccharomyces cerevisiae to obtain a first liquid strain and a second liquid strain; adding maize flour, ammonium sulfate and potassium dihydrogen phosphate in maize soaking water, regulating pH value, conducting steam sterilization to obtain liquid nutrient solution; evenly agitating the dried wet maize peel dreg with bran, maize steep liquor, bean pulp, rice chaff and urea together, regulating pH value to obtain starting crude base stock; evenly mixing the liquid nutrient solution and the starting crude base stock, then inoculating, fermenting, continuously agitating and supplying fresh air, simultaneously adding inorganic salt, and evenly mixing; and drying the fermented materials at the temperature lower than 55 DEG C to obtain the biologicprotein feed. According to the method, the biologic protein feed is produced by utilizing wet maize peel dreg generated in process of producing starch from maize through wet method, and maize soakingwater, so that the additional value is increased, and the cost is lowered.

The invention discloses an application process for trichodermin preventer, and belongs to application of microorganisms. The application process for the prior trichodermin preventing products has heavy labor, poor sporegermination and non-ideal colonization. The application process for the trichodermin preventer is to spread the trichodermin preventer comprising a trichodermin spore agent and a nutrient medium on the surface of moist soil to accelerate the growth and propagation of trichodermin. The implementation of the application process comprises the following steps: firstly performing irrigation, water spray, water sprinkle or drop irrigation, and infiltrating irrigation to moisten the soil; and then spreading the trichodermin preventer on the surface of the moist soil. The application process is simple and practical, saves labor and is easy to be accepted by users. The trichodermin preventer is applied by the application process, and the nutrient medium on the surface of the soil provides nutrient to the trichodermin to accelerate the growth and propagation of the trichodermin so as to prevent fungal diseases on roots and bud bases of plants and simultaneously prevent the fungal diseases on stems, leaves, flowers and fruits of the plants.

The invention discloses a complex microbial inoculum. The complex microbial inoculum is characterized by being prepared from 3 parts of bacillus subtilis, 4 parts of bacillus mucilaginosus, 2 parts of trichodermin, 3 parts of photosynthetic bacteria, 5 parts of enzyme microorganisms, 40 parts of amino acid powder, 3 parts of polypeptide bio-enzymes, 1 part of rooting powder and 39 parts of water. Through multiple nutrient ingredients such as beneficial microorganism organic matter and amino acid in the complex microbial inoculum, the biological and ecological balance of soil can be restored, the acility and alkalinity of the soil can be effectively regulated, hardening can be eliminated, and the activity, resisting performance and fertility of the soil can be improved; large, medium and micro water-insoluble nutrient elements difficult to absorb in the soil are decomposed and activated; beneficial bacteria are rapidly bred, harmful strains are gradually reduced till death, the beneficial bacteria occupy the whole ecosphere, beneficial ingredients in thalli can be decomposed into organic nutrient ingredients to be absorbed by plants, and meanwhile a disease prevention effect is achieved.

The present invention relates to feed additive containing microorganism. It is made up by using trichodermin, aspergillus, actinomycetes, bacillus and saccharomyces cerevisiae Li1012 through the processes of pure strain amplification culture, liquid fermentation culture and solid two-stage fermentation culture.

The invention discloses a pyrimethanil-containing bactericide composition. The active ingredients of the bactericide composition consist of trichodermin and pyrimethanil, wherein the mass ratio of the trichodermin to the pyrimethanil is (1-50):(99-50). Furthermore, the bactericide composition consists of the following ingredients by weight percent: 10-40% of the active ingredients of the bactericide composition, 10-20% of a solvent, 4-8% of an emulsifying agent, 1-3% of a dispersing agent, 1-3% of an antifreezing agent, 0.5% of a thickening agent, 0.5-1.5% of a synergistic agent, 1% of a stabilizing agent and the balance of water. The bactericide composition has an obvious synergism effect, defects generated when the two preparations are separately used can be solved, the medicine applying time and medicine applying amount can be reduced, phytotoxicity which is caused to separately apply the trichodermin to plants is eliminated, and the bactericide composition in particular has a strong effect in pathogenic bacteria with medicine resistance.

The invention discloses trichodermin derivatives, which have a structural general formula, wherein in the formula, R1 is -Me, and R2 is R3COO-; or R1 is -CHO, and R2 is H or R3COO-. The invention also discloses application of the trichodermin derivatives in killing plant pathogenic fungi which comprise sclerotinia scleotiorum, rice blast, rice false smut, rice sheath blight and the like.

A Trichoderma viride Ptri050102 (CGMCC No.1962) is prepared from the productive strain and auxiliaries through primary screening, secondary screening, culturing in natural culture medium and purifying culture. The application of said Trichoderma viride Ptri050102 in preparing Pu'er tea to control the ratio of tea polyphenol to tea brownin and improve its quality is also disclosed.

The invention discloses an agricultural ecological probiotic composite microbial inoculant which is composed of Bacillus, Acinetobacter, Trichoderma reesei, Candida tropicalis, Lactobacillus rhamnosus, Clostridium butyricum, photosynthetic bacteria, pseudomonas fluorescens, trichodermin, Paracoccus denitrificans, a carrier and a protective agent. The effective viable count of each strain is greater than 10<6> / g. By screening multiple non-antagonistic microbial strains to establish the dominant flora, the agricultural ecological probiotic composite microbial inoculant can avoid soil-borne diseases, overcome the continuous cropping obstacle, restore the soil organism ecological balance, effectively regulate the soil pH, eliminate hardening and enhance the soil activity, stress tolerance and soil fertility. The agricultural ecological probiotic composite microbial inoculant decomposes and activates major, minor, trace and other water-insoluble low-absorptivity nutrient elements in soil, increases the absorptivity of crops for nitrogen, phosphorus and potassium, greatly enhances the fertilizer efficiency, lowers the pesticide consumption, improves the quality, appearance and flavor of the agricultural products, effectively degrades agricultural residues and lowers the chemical damage.

The invention relates to a method producing trichodermin prophylactic by utilizing remained activated sludge and agriculture and forestry scrap biomass, which belongs to the technical field of biopesticide and environmental pollution treatment. The method mainly takes the remained activated sludge and scrap biomas generated during sewage disposal as the producing raw material and producing the trichodermin prophylactic by adopting the method of solid state fermentation. The manufacturing technique comprises: (1), crushing and pretreating scrap biomass; (2), preparing culture medium of sludge and biomass; (3), inoculating trichoderma viride after sterilizing the culture medium for solid state fermentation; (5), appending stabilizer and auxiliary agent into the leavening after being dried. The method can be used for crushing and packing and also can be directly used in agricultural soils, which can prevent and cure soil-borne disease and improve soil and increase soil fertility. The trichodermin prophylactic produced with the technics can effectively prevent and cure various diseases caused by plant pathology with low producing cost; besides, the product can effectively treat the castoff, thus reducing the pollution for environment.

The present invention provides use of rhizoctonia solani mycelium in the course of improving trichodermin level generated by Trichoderma harzianum, belonging to microbial technology field. The invention enhances the concentration of the trichodermin in the broth by adding inactivated rhizoctonia solani mycelium in the Trichoderma harzianum culture medium.

The invention belongs to the technical field of farm chemicals, and relates to a composition comprising brevibacillus brevis TW and trichodermin for preventing soil-borne diseases of melons, fruits and vegetables. The composition is wettable powder, which comprises 1 billion to 100 billion CFU / g of brevibacillus brevis TW and 10 million to 10 billion CFU / g of trichodermin; and the composition also comprises other ingredients in percentage by weight such as: 1 to 40 percent of dispersant, 1 to 40 percent of wetting agent and the balance of filling materials. The composition has the advantages that: (1) a good effect on preventing verticillium wilt and root rot of strawberries and blight and damping-off of vegetables can be realized; (2) the lasting period for the verticillium wilt and root rot of strawberries and the blight and damping-off of vegetables is long, so the times of the drug application can be reduced, and the prevention cost can be reduced; and (3) the composition is micro-organism combined preparation, has no chemical residue and is safe to use.

Fusarium graminearum is a plant pathogen, attacking a wide range of plant species including corn (ear and stalk rot), barley, and wheat (head blight). Fusarium epidemics result in millions of dollars of losses in crop revenues. Fusarium graminearum infection in the cereals reduces both grain yield and quality. Mycotoxins are produced by many fungal Fusarium species and thus the grain becomes contaminated with these mycotoxins, such as the trichothecenes. The major trichothecene produced by F. graminearum is deoxynivalenol (abbreviated as DON, also known as vomitoxin). Trichothecenes are potent protein synthesis inhibitors and are quite toxic to humans and livestock. A yeast gene has been identified which confers upon yeast tolerant of the trichothecene, trichodermin. A corresponding plant gene has been prepared, which has been used to transform plants. These transformed plants have an increased resistance to Fusarium infestation.

The invention relates to an ecological farming and forestry soil remediation treating agent. The ecological farming and forestry soil remediation treating agent is prepared from the following components in parts by mass: 16-20 parts of potassium indolebutyrate, 18-22 parts of calcium sulfate, 14-18 parts of cane molasses, 12-16 parts of sea mud, 18-22 parts of bacillus subtilis, 14-18 parts of trichodermin, 12-16 parts of tetramethylthiuram disulfide, 18-22 parts of malathion, 14-18 parts of zinc sulfate, 12-16 parts of trichlorfon, 18-22 parts of cloxyfonac, 14-18 parts of gibberellin, 14-18 parts of calcium ammonium nitrate, 12-16 parts of fulvic acid, 18-22 parts of manganese sulfate, 14-18 parts of calcium magnesium phosphate (fertilizer), 12-16 parts of corn stalks, 18-22 parts of bran, 14-18 parts of rutin, 12-16 parts of tea grounds, 18-22 parts of magnesium sulfate, 14-18 parts of rice bran, 12-16 parts of calcium hydrogen phosphate dihydrate, 18-22 parts of attapulgite mineral powder, 14-18 parts of nanometer aluminum silicate, 14-18 parts of triethanolamine and 14-18 parts of thiocyano amide. The ecological farming and forestry soil remediation treating agent has predominant soil remediation capacity.

The invention discloses a method of promoting the Trichodermin production level of Trichoderma harziarum and belongs to the field of microorganism technology. The method promotes the concentration of Trichodermin in fermentation broth by adding the inactivated Rhizoctonia solani mycelium into the culture medium of Trichoderma harziarum.

The invention belongs to the field of bactericide preparation, and relates to a series of trichodermin C8 oxime ether derivatives with bacteriostatic activity on plant pathogenic fungi. Specifically, the invention discloses trichodermin C8 oxime ether derivatives, and the structural general formula of the trichodermin C8 oxime ether derivatives is shown in the specification. The invention also discloses a preparation method of the trichodermin C8 oxime ether derivatives. In the trichodermin C8 oxime ether derivatives, the oxime ether structure is used as an efficient molecular efficacy group, and the parent structure of trichodermin is subjected to functional group transformation so as to prepare a series of trichodermin C8 oxime ether derivatives. The trichodermin C8 oxime ether derivatives can be used as a bactericide for the agriculture and gardening to suppress the plant pathogenic fungi.

The invention discloses active bamboo powder as well as a preparation method and an application method thereof, which relates to the field of bamboo resource utilization and environmental ecology. Theactive bamboo powder is prepared from the following raw materials in parts by weight: 100 parts of bamboo powder, 0.001 to 10 parts of active bacillus subtilis powder, 0.001 to 10 parts of active trichodermin powder, 0.001 to 10 parts of active lactobacillus powder, and 10 to 500 parts of water. The active bamboo powder can be used for promoting the growth of plants, improving the immunity of theplants, increasing the content of organic matters in soil and increasing the number of root beneficial microorganisms, has the advantages of long period of validity, low cost and the like, and is nontoxic to insects, natural enemies, human beings and animals, pollution-free to the environment and suitable for being popularized to use in the aspects of bamboos, fruit trees, tea gardens, vegetables, crops and the like.

The invention discloses a culture medium for producing trichodermin by trichoderma harziarum strain L through fermentation, and belongs to the technical field of microorganisms. The method comprises the following steps of: adding inactivated Fusarium oxysporiumf.sp.cucumeris mycelium powder into a culture medium for the trichoderma harziarum strain L for fermentation culture; centrifuging fermentation liquor; and analyzing the content of the trichodermin in the fermentation liquor by adopting a gas chromatogram, wherein the concentration of the trichodermin in the fermentation liquor after the inactivated Fusarium oxysporiumf.sp.cucumeris mycelium powder is added is improved by several times than that of a contrast. The invention provides a feasible method for improving the fermentation level of producing the trichodermin of the trichoderma harziarum strain L.

The preparation method of organic ecological soilless culture medium belongs to the field of agricultural bio technology. It is made up by using 100 portions of straw powder, 50-60 portions of cumulose fermented livestock and fowl dried excrements and 3-5 portions of compound bacterial preparation formed from azotogen and trichoderma preparation through the processes of uniformly mixing and stirring. The preparation of azotogen adopts cellulose Nocardia HD-86 with functions of decomposing cellulose and fixing nitrogen as strain and adds the corn stover, bran and water, and utilizes the aseptic fermentation process to obtain the azotogen, and the preparation of trichoderma preparation uses pseudo-Kan's trichodermin S28 as strain, and uses cob, bran, ammonium sulfate as culture medium through the processes of pulverizing, mixing, moistening, inoculating S28 and fermentation so as to obtain the trichoderma preparation. Said invention is favorable for industrial production of vegetables.

The invention relates to the technical field of cultivation of edible fungi and particularly discloses a culture medium for pleurotus eryngii and a preparation method thereof. The culture medium for pleurotus eryngii comprises the following materials in parts by weight: 20-30 parts of apple tree branch, 20-30 parts of corn straw, 15-20 parts of rice straw, 10-15 parts of bagasse, 10-20 parts of bran, 5-10 parts of rice bran, 4-8 parts of corn flour, 1-2 parts of quick lime, 0.5-1 part of urea and 0.5-1 part of gypsum powder, and also comprises a composite bacterial agent for fermentation. Thecomposite bacterial agent comprises trichodermin, bacillus subtilis, aspergillus and mycobacterium. The pleurotus eryngii is cultured by adopting waste resources such as the apple tree branch, the corn straw, the rice straw and bagasse and the like as main materials, not only is the yield high, but also the quality is good, and the economic benefit and the ecologic benefit are extremely high.

The invention discloses an environment-friendly restoring agent for soil and a preparation method thereof. The environment-friendly restoring agent is prepared by the following components in parts by weight: 5-14 parts of brown algae, 4-11 parts of bromelain, 5-17 parts of triiodobenoic acid, 3-10 parts of peat moss, 2-7 parts of trichodermin, 1-5 parts of alkyl glycoside, 3-11 parts of dried sweet potato, 2-7 parts of ethyl allicin and 2-11 parts of compound sodium nitrophenolate. The invention also discloses the preparation method of the environment-friendly restoring agent for the soil. The preparation method comprises the following detailed steps of: adding the brown algae, the bromelain, the triiodobenoic acid, the peat moss, the trichodermin, the alkyl glycoside, the dried sweet potato, the ethyl allicin and the compound sodium nitrophenolate into a mixing machine according to the parts by weight, and mixing uniformly to prepare the environment-friendly restoring agent. The environment-friendly restoring agent disclosed by the invention has the advantages that the ecological restoration effect is good and the injury to a human body is avoided and the like.

The invention discloses a method for preparing a pine-cypress substrate bacterial fertilizer. The method comprises the steps of: implementing fermenting preparation indoors at the room temperature of 5-30 DEG C; airing and grinding cypress seed husks and sesame oil residues; passing the ground cypress seed husks and the sesame oil residues through a sieve net with fineness being 800 microns; evenly mixing and stirring the cypress seed husks and the sesame oil residues by a weight ratio of 2.5:1 to form a culturing medium; adding actinomycetes and trichodermin into the culturing medium, wherein the contents of the actinomycetes and the trichodermin are respectively 0.2 billion per gram and the weight ratio of the culturingmedium to the actinomycetes to the trichodermin is 40:2:1; evenly mixing and stirring the actinomycetes, the trichodermin and the culturing medium; adding clean water and evenly stirring to obtain mixed materials, wherein the water content is 70-75%; piling up the mixed materials; covering the mixed material pile with a thin plastic film; covering the thin plastic film with a light-proof straw or hair felt and then implementing fermentation; turning the pile when a white mildew layer occurs on the surface of the culturing medium; turning the pile again when another white mildew layer occurs on the surface of the culturing medium; preparing a finished product until the culturing medium becomes black brown. The method is simple and easy to master, and the product has the advantages of evading soil pests, adsorbing heavy metal elements from soil, supplementing organic substances in the soil, improving the physical and chemical properties of the soil, improving the permeability of the soil, and the like.

An agricultural chemical 'Viridin-dimethomorph' in the form of dispersing tablet or particle is proportionally prepared from viridin, dimethomorph, adhesive, disintegrant, wetting agent, disperser and filler through fermenting viridin, mixing with the sieved mixture powder of others, and tabletting or granulating. Its advantage is no environmental pollution.

The invention discloses an environment-friendly soil restorer, specifically belonging to the technical field of soil restoration. The environment-friendly soil restorer comprises the following raw materials in parts by weight: nanometer silicon dioxide sol, activated carbon, brown algae, bromelain, sphagnum moss, trichodermin, ethyl allicin, amino polyhydroxy acid, plant ash, crushed wheat straw,an organic fertilizer, a coating agent, microbial fermentation bacterial liquid and water. With the environment-friendly soil restorer in the invention, pollutants such as bacteria, heavy metal ions and the like in soil can be degraded; crops can be inhibited from absorbing and accumulating heavy metals; the heavy metal content of crops is low; and crop growth is healthier. The air permeability ofthe environment-friendly soil restorer can be effectively improved; the environment-friendly soil restorer can quickly supplement lost nutritional ingredients in soil and guarantee normal growth of crops; since the plant ash and the wheat straw are used as main raw materials, the environment-friendly soil restorer is more energy-saving and environment-friendlier; and multiple variable-speed stirring and mixing of the raw materials can be realized, so the content deviation of effective substances in each particle of the soil environment-friendly restorer is smaller, and chemical effect is guaranteed.

The invention discloses an environmentally-friendly soil restorer and a preparation method thereof. The soil restorer is prepared from, by weight, 5-15 parts of corn stigmas, 6-18 parts of brown algae, 5-15 parts of bromelain, 8-20 parts of triiodobenzoic acid, 2-8 parts of sphagnum moss, 3-8 parts of trichomycin, 1-6 parts of alkyl glycosides, 6-12 parts of dried sweet potatoes, 3-8 parts of ethyl allicin and 3-10 parts of compound sodium nitrophenolate. The environmentally-friendly soil restorer prepared from the corn stigmas, brown algae, bromelain, triiodobenzoic acid, sphagnum moss, trichodermin, alkyl glycosides, dried sweet potatoes, ethyl allicin and compound sodium nitrophenolate can degrade pollutants such as bacteria and heavy metal ions in soil in order to achieve the purpose of soil remediation, has a good effect, effectively avoids damages to human bodies, and has a good environmentally-friendly effect.