Biocontrol trichoderma agent prepared with active sludge and abandoned biomass
A technology for waste biomass and biocontrol agents, applied in the fields of biological pesticides and environmental pollution control, can solve the problems of high energy consumption in the heating process, and achieve the effect of low production cost
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Embodiment 1
[0013] Activated sludge and corn stover fermentation Trichoderma viride:
[0014] After crushing the corn stalks, mix them evenly with the dehydrated activated sludge according to the weight ratio of 2:1, adjust the water content to 75% with water, and sterilize at 120°C for 20 minutes. 6 spores / gram medium inoculum inoculation, at 30 ℃, static culture for 6 days. The spore content of the cultivated material can reach 3.2×10 9 spores per gram of substrate, after drying, adding stabilizers and auxiliary agents, pulverizing, and packaging.
Embodiment 2
[0016] Activated Sludge and Bagasse Fermentation Trichoderma harzianum:
[0017] Mix bagasse and dewatered activated sludge evenly according to the weight ratio of 3:2, adjust the water content to 75% with water, and sterilize at 120°C for 20 minutes, according to 8×10 6 spores / gram medium inoculum inoculation, at 30 ℃, static culture for 6 days. After the cultivated materials are dried, stabilizers and additives are added, crushed and packaged. The spore concentration of the product reaches 1.1×10 10 spores / gram.
Embodiment 3
[0019] Co-fermentation of Trichoderma harzianum and Trichoderma konii with activated sludge and sugar beet pulp:
[0020] Mix sugar beet pulp and dehydrated activated sludge evenly according to the weight ratio of 1:1, adjust the water content to 75% with water, and sterilize at 120°C for 20 minutes. 6 Trichoderma harzianum and Trichoderma konii were respectively inoculated with the inoculation amount of 1 spore / gram of culture medium, and cultured statically for 5 days at 30°C. After the cultivated materials are dried, stabilizers and additives are added, crushed and packaged.
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