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Biocontrol trichoderma agent prepared with active sludge and abandoned biomass

A technology for waste biomass and biocontrol agents, applied in the fields of biological pesticides and environmental pollution control, can solve the problems of high energy consumption in the heating process, and achieve the effect of low production cost

Inactive Publication Date: 2008-01-23
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent (publication number: CN 1493535) discloses a method of using activated sludge to prepare activated sludge carbon. The method is to process activated sludge at 200-700°C for 0.5-2 hours to obtain activated sludge. Peat, the prepared activated sludge charcoal has better activated carbon performance, and the heating process consumes more energy
And the method of producing Trichoderma biocontrol agent with activated sludge and waste biomass as culture medium has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Activated sludge and corn stover fermentation Trichoderma viride:

[0014] After crushing the corn stalks, mix them evenly with the dehydrated activated sludge according to the weight ratio of 2:1, adjust the water content to 75% with water, and sterilize at 120°C for 20 minutes. 6 spores / gram medium inoculum inoculation, at 30 ℃, static culture for 6 days. The spore content of the cultivated material can reach 3.2×10 9 spores per gram of substrate, after drying, adding stabilizers and auxiliary agents, pulverizing, and packaging.

Embodiment 2

[0016] Activated Sludge and Bagasse Fermentation Trichoderma harzianum:

[0017] Mix bagasse and dewatered activated sludge evenly according to the weight ratio of 3:2, adjust the water content to 75% with water, and sterilize at 120°C for 20 minutes, according to 8×10 6 spores / gram medium inoculum inoculation, at 30 ℃, static culture for 6 days. After the cultivated materials are dried, stabilizers and additives are added, crushed and packaged. The spore concentration of the product reaches 1.1×10 10 spores / gram.

Embodiment 3

[0019] Co-fermentation of Trichoderma harzianum and Trichoderma konii with activated sludge and sugar beet pulp:

[0020] Mix sugar beet pulp and dehydrated activated sludge evenly according to the weight ratio of 1:1, adjust the water content to 75% with water, and sterilize at 120°C for 20 minutes. 6 Trichoderma harzianum and Trichoderma konii were respectively inoculated with the inoculation amount of 1 spore / gram of culture medium, and cultured statically for 5 days at 30°C. After the cultivated materials are dried, stabilizers and additives are added, crushed and packaged.

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PUM

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Abstract

The invention relates to a method producing trichodermin prophylactic by utilizing remained activated sludge and agriculture and forestry scrap biomass, which belongs to the technical field of biopesticide and environmental pollution treatment. The method mainly takes the remained activated sludge and scrap biomas generated during sewage disposal as the producing raw material and producing the trichodermin prophylactic by adopting the method of solid state fermentation. The manufacturing technique comprises: (1), crushing and pretreating scrap biomass; (2), preparing culture medium of sludge and biomass; (3), inoculating trichoderma viride after sterilizing the culture medium for solid state fermentation; (5), appending stabilizer and auxiliary agent into the leavening after being dried. The method can be used for crushing and packing and also can be directly used in agricultural soils, which can prevent and cure soil-borne disease and improve soil and increase soil fertility. The trichodermin prophylactic produced with the technics can effectively prevent and cure various diseases caused by plant pathology with low producing cost; besides, the product can effectively treat the castoff, thus reducing the pollution for environment.

Description

technical field [0001] The invention belongs to the technical field of biological pesticide and environmental pollution control. technical background [0002] In the sewage treatment process, the activated sludge method is one of the most widely used technologies. This method plays a very important role in water treatment because of its advantages of high efficiency, low cost and less land occupation. However, this method During the process of sewage treatment, a large amount of residual sludge will be produced, usually accounting for 0.5% to 1.0% of the total sewage. In an ordinary secondary sewage treatment plant, the investment required for sludge treatment accounts for about 30% to 40% of the total investment, and the operating cost accounts for about 20% to 50% of the total operating cost. Sludge digestion, dehydration, and then landfill is a commonly used disposal method in domestic large-scale sewage treatment plants. Landfill disposal is more economical, but this d...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P1/00
Inventor 白志辉张保国呼庆齐鸿雁张洪勋
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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