Pyrimethanil-containing bactericide composition
A fungicide composition, pyrimethanil technology, applied in the direction of fungicides, biocides, biocides, etc., can solve the problems of application restrictions, high production costs, etc., to reduce usage and water consumption, reduce labor costs, delay The effect of drug resistance
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Embodiment 1
[0059] Example 1 The joint toxicity test of pyrimethanil and trichoderma
[0060] 1. Drugs to be tested:
[0061] Trichomycin: can be provided by Zhejiang Dahua Biotechnology Co., Ltd.;
[0062] Pyrimethanil: It can be provided by Bayer Crop Science of Bayer (China) Co., Ltd., Germany.
[0063] 2. Test strains:
[0064] The pyrimethanil-resistant strain, Botrytis cinerea Pers (Botrytis cinerea Pers), was provided by Professor Ma Zhonghua, Institute of Biotechnology, Zhejiang University.
[0065] 3. Medium:
[0066] Potato dextrose agar (PDA) 330126197404302310
[0067] 4. Method
[0068]The virulence coefficients of trichoderma, pyrimethanil and their compound agents against resistant Botrytis cinerea were tested by mycelial growth rate method. The mass ratios of compound medicaments are trichomycin and pyrimethanil are 1:99, 5:95, 10:90, 20:80, 30:70, 50:50, 70:30, 80:20, 90: 10. Dilute each test agent with sterile water into a series of gradient concentrations, and th...
Embodiment 2
[0082] Example 2 In vitro test of drug efficacy of compound preparation
[0083] 1 Test agent:
[0084] Composite medicament: the mass ratio of trichomycin and pyrimethanil is 10:90 (combined toxicity test results show in embodiment 1 that the mass ratio of trichomycin and pyrimethanil 10:90 is compounded and the co-toxicity coefficient is 171.7 , significant synergistic effect, comprehensive agent cost and synergistic ratio, trichomycin and pyrimethanil 10:90 is the best ratio, so the following drug efficacy tests are based on the mass ratio of trichomycin and pyrimethanil 10:90 for compounding).
[0085] 2 Test crops:
[0086] The tomato variety is the cooperative 903 red tomato provided by the Shanghai Long March Fine Variety Experimental Field of the Shanghai Tomato Research Institute, which is commercially available and has a batch number of 20091106. Tomato seeds were soaked and sterilized with potassium permanganate, germinated in an incubator at 28°C for 48 hours, a...
Embodiment 3
[0100] Example 3 Potted drug efficacy test of compound preparation
[0101] 1 Test agent:
[0102] Compound drug: the mass ratio of trichomycin and pyrimethanil is 10:90.
[0103] 2 Test crops:
[0104] The tomato variety is the cooperative 903 red tomato provided by the Shanghai Long March Fine Variety Experimental Field of the Shanghai Tomato Research Institute, which is commercially available and has a batch number of 20091106. Tomato seeds were soaked and sterilized with potassium permanganate, germinated in an incubator at 28°C for 48 hours, and then seedlings were raised in seedling trays, and transplanted into pots with a diameter of 10 cm after the three leaves were transplanted, one plant per pot, and the five-leaf stage was used for test.
[0105] 3 methods:
[0106] The experiment consisted of 4 treatments
[0107] Treatment 1: Trichoderma 10% aqueous emulsion, diluted 1000 times;
[0108] Treatment 2: Pyrimethanil 40% suspension, diluted 1000 times;
[0109]...
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