The invention discloses a method for detecting
ochratoxin A (OTA) based on
luminescence resonance energy transfer between an up-conversion
luminescence nano-material and gold nano-rods. The method is used for detecting the content of OTA in wheat and products thereof. The up-conversion
luminescence nano-material (NaYF4:Yb0.286, Er0.0286) and OTA aptamers are connected to form an energy donor probe, then the energy donor probe and an energy
receptor probe form a nano-composite based on the base complementary
pairing principle, the phenomenon of luminescence
resonance energy transfer (LRET) occurs, and the purpose of up-conversion luminescence
quenching is achieved, wherein the energy
receptor probe is formed by the gold nano-rods modified by
aptamer complementary
oligonucleotide single strands, and the
aspect ratio of the gold nano-rods is about 2.5. When OTA exists in a detection
system, OTA and OTA aptamers are specifically bound, and therefore double strands are melted; OTA can be quantitatively detected by monitoring the up-conversion luminescence
signal intensity at 657 nm, the
linearity range is 0.05-100 ng / mL, and the
detection limit is 0.027 ng / mL. The method has the advantages of being high in sensitivity, fast, simple and convenient to implement when used for detecting OTA. In addition, the method is applied to detecting beer or wheat samples, and results are accurate and reliable.