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Biosensor for detecting zearalenone and ochratoxin A and preparation method thereof, and method for detecting zearalenone and ochratoxin A

A technology of zearalenone and ochratoxin, which is applied to the biosensor for simultaneous detection of zearalenone and ochratoxin A, can solve the problem of cost Long time, complicated operation steps, high detection cost and other problems, to achieve the effect of low cost, good specificity and high sensitivity

Active Publication Date: 2019-09-20
QINGDAO AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of cumbersome single detection method of ZEN and OTA in the prior art, complicated operation steps, high detection cost and long time spent, the present invention provides a fluorescent aptamer using graphene oxide as a fluorescent quencher Sensor, and method for simultaneously detecting ZEN and OTA using the sensor

Method used

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  • Biosensor for detecting zearalenone and ochratoxin A and preparation method thereof, and method for detecting zearalenone and ochratoxin A
  • Biosensor for detecting zearalenone and ochratoxin A and preparation method thereof, and method for detecting zearalenone and ochratoxin A
  • Biosensor for detecting zearalenone and ochratoxin A and preparation method thereof, and method for detecting zearalenone and ochratoxin A

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Effect test

Embodiment

[0037] (1) ZEN aptamer and OTA aptamer self-assemble with graphene oxide to form a detection platform

[0038] Pretreatment: ZEN aptamer and OTA aptamer were respectively treated with PBS buffer (NaCl 136.89mM, KCl2.67mM, NaCl 2 HPO 4 8.1mM,KH 2 PO 4 1.76Mm, pH=7.4) dissolved to a concentration of 1 μM, placed at 4°C and protected from light with tinfoil. The concentration of ZEN aptamer and OTA aptamer working solution was 1 μM. The purchased 500 μg / mL graphene oxide suspension was diluted with ultrapure water to a concentration of 250 μg / mL, ultrasonicated at 200 W for 1 h, and placed at 4 °C for use.

[0039] Add 20 μL of ZEN aptamer (1 μM) and 20 μL of OTA aptamer (1 μM) into 20 μL of GO with a concentration of 20 μg / mL, shake well and incubate at room temperature for 10 min to make ZEN aptamer and OTA The aptamer is fully combined with the graphene oxide, and the fluorescence of the ZEN aptamer and the OTA aptamer is completely quenched by the graphene oxide, formi...

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Abstract

The invention discloses a biosensor for detecting zearalenone and ochratoxin A and a preparation method thereof, and a method for detecting zearalenone and ochratoxin A, and belongs to the technical field of harmful substance detection. The biosensor for detecting zearalenone is formed by self-assembling a ZEN aptamer which is a nucleic acid aptamer capable of specifically recognizing zearalenone and labeled by a fluorescent group at the 5' end and graphene oxide. The biosensor can detect ZEN and OTA at the same time, overcomes the problems that the scheme of a single detection method is tedious, the operation steps are complex, the detection cost is high and the required time is long. The two types of fungaltoxins are detected at the same time and are not mutually interfered, and the accuracy is high.

Description

technical field [0001] The invention belongs to the technical field of detection of harmful substances, and in particular relates to a biosensor for simultaneously detecting zearalenone and ochratoxin A, a preparation method thereof, and a method for using the sensor to simultaneously detect zearalenone and ochratoxin A . Background technique [0002] Mycotoxins are a large class of toxic secondary fungal metabolites produced by filamentous fungi. When ingested above a certain concentration of mycotoxins, it can cause a toxic reaction called mycotoxin poisoning, which can also lead to teratogenicity, carcinogenicity and mutagenicity. Zearalenone (ZEN) is a mycotoxin with estrogen-like effects produced by a variety of Fusarium. Zearalenone has strong reproductive toxicity and teratogenic effects. There is a great harm to organ function and so on. Ochratoxin A (OTA) is a class of secondary metabolites mainly produced by fungi such as Aspergillus and Penicillium. It is the m...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486
Inventor 杨庆利王琦吴薇颜春蕾都晗赵海燕于春娣朱英莲
Owner QINGDAO AGRI UNIV
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