40 results about "Reproductive toxicity" patented technology

A drug screening method includes the steps: A, screening PPARs (peroxisome proliferator-activated receptors) and building a PPAR action model; B, building a compound database; C, evaluating druggability, LogP, LogS, mutagenicity, tumorigenicity, irritation, reproductive toxicity and water solubility of compounds in the database, and building two-dimensional and three-dimensional compound databases; D, performing structural modification on compounds in the two-dimensional and three-dimensional compound databases; E, performing docking computation on novel molecular structural compounds, and screening; F, verifying the compounds by the aid of a molecular dynamics method, and selecting and determining molecules; and G, obtaining ADMET (absorption, distribution, metabolism, excretion and toxicity) properties of the verified compounds obtained by computation so as to obtain target compounds finally. By the scheme, structures of the compounds are further optimized while structure-function relationship of the compounds is researched, and novel leading compounds with medicinal value are sought.

The present invention discloses a reproductive-toxicity-free polyvinyl chloride vehicle interior decoration leather and a preparation method thereof, wherein the reproductive-toxicity-free polyvinyl chloride vehicle interior decoration leather comprises a polyvinyl chloride surface layer, a polyvinyl chloride foaming layer and an aqueous paint layer coated on the polyvinyl chloride surface layer. According to the present invention, the aqueous paint layer does not contain N-methylpyrrolidone and N-ethylpyrrolidone, and does not have reproductive toxicity under the premise of the meeting of the environmental protection requirements of low VOC and low odor, and the surface performance requirements of wear resistance, illumination resistance, heat resistance, fouling resistance and the like so as to avoid the harm to human body.

The invention provides an antimicrobial alginate fiber as well as a preparation method and application of an antimicrobial alginate fiber dressing. According to the invention, the technical difficult problem that the antimicrobial activity of guanidine salt is quickly passivated after polyhexamethyleneguanidine salt as a cationic polymer is mixed with alginate as an anionic polymer is solved, and good and long-term antimicrobial activity of a polyhexamethyleneguanidine alginate fiber with relatively-low content and a medical product are realized. The polyhexamethyleneguanidine salt is low in dissolution rate and provides good safety, so that potential risks of carcinogenesis, mutagenesis and reproductive toxicity are remarkably reduced.

Applications of cyanidin-3-O-glucoside in preparation of medicines treating and / or preventing diseases caused by 3-chloropropane-1,2-diol are provided. The cyanidin-3-O-glucoside (C3G) can improve rat ingestion decrease and growth retardation which are caused by 3-MCPD poisoning, can effectively resist rat antioxidant system damage caused by 3-MCPD poisoning, and can activate and adjust BTB structural functions of sustentacular cells through inhibiting an MAPK signaling pathway, thus relieving seminiferous epithelium injuries caused by the 3-MCPD, maintaining stability of a spermatogenic microenvironment, regulating spermatogenic cell proliferation and differentiation, and improving dyszoospermia. In addition, the C3G can improve sperm activity through adjusting sperm energy metabolism related enzymes. The C3G can regulate rat sex hormone secretion disorders caused by 3-MCPD poisoning and can maintain normal levels of sex hormone in serum and androgen in testis inner environments. The applications provide theoretical foundations for 3-MCPD reproduction toxicity nutritional intervention utilizing anthocyanin.

The invention discloses application of cyanidin-3-O-glucoside in preparation of drugs for treating and / or preventing hormonal disorder diseases. It is found that the cyanidin-3-O-glucoside can improvethe androgen secretion disorder caused by Cd exposure during puberty, and the hypothalamus-pituitary-gonad axis can be protected from injuries caused by the Cd exposure. According to C3G intervention, the synthesis and secretion of hypothalamic gonadotropin releasing hormones, the expression of pituitary and testicular sex hormone receptors and the expression of testosterone synthesis pathway-related proteins in the testis can be intervened, so that the testosterone concentration returns to a normal level, the androgen level in the inner environment of the testis is maintained accordingly, and the generation of sperms is promoted. The application provides a theoretical basis for future research on the nutrition intervention of anthocyanins with the Cd reproductive toxicity and has an important significance.

The invention discloses application of cyanidin-3-O-glucoside in preparing medicine for preventing and / or treating male genital diseases caused by 1,3-dichloro-2-propanol. Research results show that the C3G has a significant inhibitory effect on R2C cell activity decline, cell morphological damage, increase in cell apoptosis rate and the like exposed by 1,3-DCP, the C3G, by promoting the expression of StAR, 3beta-HSD and like key protein, intervenes in R2C cell progesterone synthesis exposed by the 1,3-DCP and simultaneously the C3G, by reducing ROS (reactive oxygen species) inside the R2C cell exposed by the 1,3-DCP and further inhibiting MMP (mitochondrial membrane potential) and the like, intervenes in R2C cell apoptosis and progesterone expression function injury caused by the 1,3-DCP. The research achievement of the invention provides an important basis for human to prevent and treat male genital diseases caused by 1,3-dichloro-2-propanol and has a great research significance.

The invention relates to a new compound and a preparation field thereof and provides two compounds containing a quinoxaline mother rotor: 3-methyl-2-ethoxycarbonyl-quinoxaline-1, 4-dioxide (a compound I) and 2-acetyl-3-phenyl-quinoxaline-1, 4-dioxide (a compound II) and a preparation method thereof. The existing quinoxaline medicaments are widely applied to the antisepsis and growth promotion of animals, but such problems as medicament residual, reproductive toxicity and other aspects appear if the medicaments are applied in a long run. The two quinoxaline compounds have the advantages of strong resistance to bacteria and low toxicity. The two compounds with the quinoxaline mother rotor can be applied to the growth promotion of animals, and the preparation of antimicrobial agents, feedstuff or additives.

The invention discloses a traditional Chinese medicine composition for treating common cold and upper respiratory tract infection. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 10 to 80 parts of raw gypsum, 5 to 50 parts of forsythia suspensa, 5 to 50 parts of radix isatidis, 5 to 40 parts of fineleaf schizonepeta herb, 5 to 40 parts of fermented soybeans, 5 to 45 parts of perilla leaves, 5 to 45 parts of semen perillae acutae and 2 to 30 parts of licorice. Original efficacy of each raw material is played, and meanwhile, the raw materials interact and coordinate to achieve the effects of clearing heat, removing toxicity, relieving exterior syndromes, dispelling cold, clearing and diffusing lung heat, expelling phlegm to arrest coughing, cooling blood, relieving sore throat, and killing and inhibiting bacteria; the formula is rigorous, and the raw materials are scientifically combined, blended and supplement one another to achieve the effect of unified treatment over various types of exterior and interior cold, heat and summer damp; the root of each type of common cold is seized, disease differentiation is combined with syndrome differentiation, and the raw materials can be dialectically applied to treat various types of common cold and upper respiratory tract infection. A reproductive toxicity efficacy experiment on the traditional Chinese medicine composition formed by the raw materials shows that the traditional Chinese medicine composition is free of remarkable influence on a pregnant rat and a fetal rat, and can be applied to the treatment of common cold of pregnant women and infants.

A coating composition comprises: (a) a resin solution containing a polyamide-imide resin and a solvent having a urea bond; and (b) a powdered solid lubricant. In general, the coating composition containing a polyamide-imide resin does not raise any concern about reproductive toxicity and has excellent lubrication durability.

The invention discloses application of prosaposin (psap) serving as an effect marker in detection of the male reproductive toxicity of polychlorinated biphenyl, and relates to psap. The psap is multifunctional glycoprotein, has the molecular weight of 70kD, and exists in some biological fluids. The method for screening the psap comprises the following steps of: exposing a small mouse by using different quantities of polychlorinated biphenyl, and acquiring a sample; screening difference expression genes of the sample; performing function accumulation on the screened difference expression genes to obtain a difference expression gene relevant to a reproductive function; detecting and analyzing the change of the obtained difference expression gene on an mRNA transcription level and a protein expression level by using a real-time fluorescent quantitative polymerase chain reaction (PCR) technology and an immunoblotting method along with the increase of a polychlorinated biphenyl (PCB) exposed amount and the exposing time; detecting and analyzing sperm parameters; and counting relevant coefficients among the effect marker, an in-vivo polychlorinated biphenyl level and a semen index through Spearman correlation analysis.

The invention belongs to the technical field of biology, relates to a detection method for detecting reproduction toxicity of BBP (Butyl Benzyl Phthalate), in particular relates to a detection method for detecting the reproduction toxicity of BBP by utilizing model organism zebra fishes and specifically relates to a method for rapidly detecting the male sperm production capability and female egg production capability of BBP by utilizing adult zebra fishes. According to the method disclosed by the invention, the experimental result displays that the detection period is only 8 days and is shorter than that of other methods reported in the existing literature; and moreover, the lowest concentration of the detected reproduction toxicity of BBP is 0.6mg.L<-1> and is superior to the detected concentration in other methods.

The invention discloses application of cyanidin-3-O-glucoside in preparing medicines for preventing and / or treating male genital diseases caused by acrylamide and / or glycidamide. Based upon research results, C3G (the cyanidin-3-O-glucoside) can take an effective intervention effect on injury of R2C cells caused by AA or GA and can inhibit R2C cell oxidative stress functional lesion caused by the AA or GA, so that the survival rate of the cells is increased and progesterone synthesis is improved. The C3G can take a relatively good intervention protective effect on R2C cell activity declination, cell apoptosis, progesterone function expression injury and the like exposed by the AA or GA, and the optimum intervention concentration is 10-50[mu]M (M refers to mol / L). Upon research results, the cyanidin-3-O-glucoside provides a novel application;and moreover, the cyanidin-3-O-glucoside provides an important basis for preventing and treating the male genital diseases caused by the AA or GA in human bodies; therefore, the cyanidin-3-O-glucoside has an important research significance.

The invention provides an antimicrobial alginate fiber as well as a preparation method and application of an antimicrobial alginate fiber dressing. According to the invention, the technical difficult problem that the antimicrobial activity of guanidine salt is quickly passivated after polyhexamethyleneguanidine salt as a cationic polymer is mixed with alginate as an anionic polymer is solved, and good and long-term antimicrobial activity of a polyhexamethyleneguanidine alginate fiber with relatively-low content and a medical product are realized. The polyhexamethyleneguanidine salt is low in dissolution rate and provides good safety, so that potential risks of carcinogenesis, mutagenesis and reproductive toxicity are remarkably reduced.

The invention provides an application of C3G in preparation of an antagonist for resisting oxidation system damage caused by 3-MCPD contamination. The invention proves that C3G improves the conditions of reduced feeding and slow growth of rats caused by 3-MCPD contamination; the C3G can effectively antagonize rat anti-oxidation system damage caused by 3-MCPD contamination and activate and regulate BTB structure functions of supporting cells by inhibiting an MAPK signal channel, so that spermatogenic epithelium damage caused by 3-MCPD is relieved, the stability of a spermatogenic microenvironment is maintained, division and differentiation of spermatogenic cells are regulated, and spermatogenic disorder is improved. Meanwhile, C3G can improve sperm activity by adjusting sperm energy metabolism related enzymes. Rat sex hormone secretion disorder caused by 3-MCPD contamination is regulated, and the normal level of sex hormone in serum and androgen in testis internal environment is maintained. The application of C3G in preparation of the antagonist for resisting oxidation system damage caused by the 3-MCPD contamination provides a theoretical basis for nutrition intervention of anthocyanin on 3-MCPD reproductive toxicity in the future.

The invention relates to a double-chain siRNA and application thereof, in particular to a double-chain siRNA of DNA damage repair oggl gene, which is applied to mouse testicle microenvironment knockdown gene expression for improving the sensitivity of a male reproductive system to the damage effect of environmental chemicals so as to provide a method for quickly and sensitively detecting male reproductive toxicity of environmental chemicals. As a new method for detecting the reproductive toxicity of the environmental chemicals, the method can realize sensitive and quick reflection of male reproductive hazard caused by the chemicals, and is favorable for more accurately evaluating the safety of the chemicals, and for shortening the period of pre-clinical reproductive toxicity experiments of medicaments.

The invention provides a method for evaluating reproductive toxicity of an organic solvent by utilizing drosophila melanogaster, and belongs to the technical field of toxicity research. The method comprises the following steps: when the drosophila melanogaster is placed on a solid culture medium containing the organic solvent for 10 days, observing the ovaries of female drosophila melanogaster, wherein it shows that the organic solvent has reproductive toxicity when the ovaries are abnormal. The volume concentration of the organic solvent in the solid culture medium containing the organic solvent is 0.1-1.25%. By adopting the method provided by the invention, the reproductive toxicity of the organic solvent can be accurately evaluated, and theoretical and experimental data support is provided for the dosage and concentration of the organic solvent reasonably used in agriculture.

The invention discloses a biomarker group and application thereof, and belongs to the technical field of bioengineering. By applying an RNA-seq technology, the result shows that the mRNA expression level of an mt-Nd6 gene in a male reproductive system is lowered, and meanwhile the mRNA expression level of an Slc12a4 gene in the male reproductive system is raised; and therefore, the Slc12a4 gene and the mt-Nd6 gene can serve as the biomarker group for diagnosing male reproductive toxicity caused by prochloraz. By studying the mechanism that prochloraz affects the male reproductive system, the invention provides the biomarker group for diagnosing the male reproductive toxicity caused by prochloraz, the Slc12a4 gene and the mt-Nd6 gene can provide an important basis for preventing and treating male reproductive diseases caused by prochloraz for human, and the study has practical significance.

The invention relates to a new compound and a preparation field thereof and provides two compounds containing a quinoxaline mother rotor: 3-methyl-2-ethoxycarbonyl-quinoxaline-1, 4-dioxide (a compound I) and 2-acetyl-3-phenyl-quinoxaline-1, 4-dioxide (a compound II) and a preparation method thereof. The existing quinoxaline medicaments are widely applied to the antisepsis and growth promotion of animals, but such problems as medicament residual, reproductive toxicity and other aspects appear if the medicaments are applied in a long run. The two quinoxaline compounds have the advantages of strong resistance to bacteria and low toxicity. The two compounds with the quinoxaline mother rotor can be applied to the growth promotion of animals, and the preparation of antimicrobial agents, feedstuff or additives.

The present disclosure provides an antimicrobial alginate fiber, a method for manufacturing an antimicrobial alginate fiber dressing thereof, and an application thereof. When the cationic polymer polyhexamethylene guanide salt is mixed with the anionic polymer alginate, the antimicrobial activity of the guanide salt is quickly passivated. The present disclosure achieves a lower content of polyhexamethylene guanide salt in an alginate dressing with good long-term antimicrobial activities. The polyhexamethylene guanide salt has a low dissolution rate, and significantly reduces a potential risk of carcinogenicity, mutagenicity and reproductive toxicity.

The invention relates to a pre-dispersed masterbatch formula containing tetrahydro-2(1H)-pyrimidinethione and a preparation method of the formula. The formula comprises the following components in parts by mass: 73-77 parts of tetrahydro-2(1H)-pyrimidinethione; 10-15 parts of a rubber carrier; 2-10 parts of an active agent component; 1-6 parts of hydrocarbon paraffin solid or fatty acid salt; and2-7 parts of paraffin oil. The preparation method comprises the following steps: firstly, preparing the rubber carrier; weighing the required raw materials; putting all the raw materials into an internal mixer to be internally mixed to obtain a rubber material; and finally, putting the rubber material into an extruder, and controlling the given rotating speed of a main machine to be 115-125r / min and the extrusion temperature to be 50-70 DEG C to prepare the pre-dispersed masterbatch containing the tetrahydro-2(1H)-pyrimidinethione. According to the invention, the dispersion problem of the tetrahydro-2(1H)-pyrimidinethione in rubber compound is improved, and the problem of dust pollution caused by direct use of the tetrahydro-2(1H)-pyrimidinethione is solved at the same time. Compared withrubber, especially chloroprene rubber, the pre-dispersed masterbatch has the characteristics of high vulcanization speed, high scorching safety, flat vulcanization, excellent thermal aging performance, no pollution, no coloring and no blooming, can replace ETU with reproductive toxicity and capable of generating carcinogenic substance nitrosamine in the vulcanization or use process, and has practical production value.

The invention provides a method for evaluating the heavy metal reproductive toxicity resistance effect of traditional Chinese medicine polysaccharide extract. The method comprises the following stepsof exposing caenorhabditis elegans to culture mediums of a blank group, a control group and a tested group, and evaluating the heavy metal reproductive toxicity resistance effect of the traditional Chinese medicine polysaccharide extract according to the total generation number, the egg number, the germ cell apoptosis number and the progeny growth and reproduction conditions of the caenorhabditiselegans, wherein the culture medium of the blank group comprises a buffer solution and escherichia coli; the culture medium of the control group comprises a buffer solution, escherichia coli and heavymetal ions, and the culture medium of the tested group comprises a buffer solution, escherichia coli, traditional Chinese medicine polysaccharide extract and heavy metal ions. The method for evaluating the heavy metal reproductive toxicity resistance effect of the traditional Chinese medicine polysaccharide extract provided by the invention is simple, low in cost and short in test period; and meanwhile, technical support is provided for reproduction protection research of traditional Chinese medicine polysaccharide products, and the heavy metal reproductive toxicity resistance effect of polysaccharide and other health-care foods or drugs can be evaluated.

The present invention relates to a photoresist-removing stripper composition which does not comprise N-methylformamide, N, N'-dimethylcarboxamide, or the like, showing reproductive toxicity, but is capable of showing excellent stripping capacity and rinsing capacity and is capable of minimizing time-dependent deterioration of physical properties; and a method for stripping a photoresist using the composition. The photoresist-removing stripper composition comprises: one or more types of amine compounds; N, N'-diethylcarboxamide; and a protic organic solvent of alkyleneglycol monoalkylether or alkyleneglycol.

The invention relates to an application of a pharmaceutical composition in inhibition of ras proto-oncogene overexpression, and specifically, relates to the application in preparation of anti-tumor drugs for inhibiting ras proto-oncogene overexpression. Specifically, the pharmaceutical composition contains radix ginseng, radix ophiopogonis and schisandra chinensis fruits. Researches indicate that about 90% of pancreatic cancer, 50% of colon cancer, 30%-40% of lung adenocarcinoma and 5%-40% of leukemia are caused by the ras proto-oncogene overexpression, and a Ras protein becomes a universally accepted target for screening related malignant tumor drugs. Animal experiments indicate that the pharmaceutical composition can specifically down-regulate overexpression caused after ras proto-oncogene mutation, but does not act on wild-type ras proto-oncogenes, so that the pharmaceutical composition is low in toxicity and has no teratogenic effect and no reproduction toxicity; the pharmaceutical composition can be used for preparation of drugs for tumors caused by overexpression after ras proto-oncogene mutation, can be mixed with any one of pharmaceutically acceptable auxiliary materials to prepare various dosage forms, and is expected to be developed into a new-generation antitumor drugs.

The invention provides a medicinal composition for preventing vascular thrombosis diseases. The medicinal composition consists of 4 to 16 parts of ferulic acid, 2.5 to 10 parts of protocatechuic acid,3 to 12 parts of p-coumaric acid, and 2 to 8 parts of isoferulic acid. The antithrombotic titer of the medicinal composition prepared in the invention can reach 96.66 percent. The medicinal composition of the invention comprises no toxic ingredient 5-hydroxymethyl furfural, the potential toxic harm of rhizoma sparganii crude drugs on the inheritance and reproduction of human bodies can be avoided, the medicinal composition is safe to use, and the curative effect is improved; finally, a foundation is set for further developing novel active ingredient composition preparations.

The invention discloses a traditional Chinese medicine composition for treating common cold and upper respiratory tract infection. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 10 to 80 parts of raw gypsum, 5 to 50 parts of forsythia suspensa, 5 to 50 parts of radix isatidis, 5 to 40 parts of fineleaf schizonepeta herb, 5 to 40 parts of fermented soybeans, 5 to 45 parts of perilla leaves, 5 to 45 parts of semen perillae acutae and 2 to 30 parts of licorice. Original efficacy of each raw material is played, and meanwhile, the raw materials interact and coordinate to achieve the effects of clearing heat, removing toxicity, relieving exterior syndromes, dispelling cold, clearing and diffusing lung heat, expelling phlegm to arrest coughing, cooling blood, relieving sore throat, and killing and inhibiting bacteria; the formula is rigorous, and the raw materials are scientifically combined, blended and supplement one another to achieve the effect of unified treatment over various types of exterior and interior cold, heat and summer damp; the root of each type of common cold is seized, disease differentiation is combined with syndrome differentiation, and the raw materials can be dialectically applied to treat various types of common cold and upper respiratory tract infection. A reproductive toxicity efficacy experiment on the traditional Chinese medicine composition formed by the raw materials shows that the traditional Chinese medicine composition is free of remarkable influence on a pregnant rat and a fetal rat, and can be applied to the treatment of common cold of pregnant women and infants.

The invention provides a gossypol removing method for cotton seeds. The method comprises the steps of shelling and cleaning the cotton seeds, putting the cotton seeds into deionized water in a solid-liquid ratio of 1: (3-15), keeping for 12-36khz while stirring, performing ultrasonic treatment for 10-30min under 10-50hz while stirring, washing for 3-6 times, dehydrating, sterilizing for 10-30 minutes with flowing steam, drying at 80 DEG C till the water content is 10%, thus obtaining gossypol removed cotton seeds. By adopting the mild processing method, gossypol is effectively removed, and the defects of high cost, organic solvent residue, insufficient gossypol removal and the like in present general gossypol removing methods are overcome; and the method is simple, saves energy, reduces the cost, and solves the problem of reproductive toxicity of the gossypol included in the cotton seeds.

The present disclosure provides an antimicrobial alginate fiber, a method for manufacturing an antimicrobial alginate fiber dressing thereof, and an application thereof. When the cationic polymer polyhexamethylene guanide salt is mixed with the anionic polymer alginate, the antimicrobial activity of the guanide salt is quickly passivated. The present disclosure achieves a lower content of polyhexamethylene guanide salt in an alginate dressing with good long-term antimicrobial activities. The polyhexamethylene guanide salt has a low dissolution rate, and significantly reduces a potential risk of carcinogenicity, mutagenicity and reproductive toxicity.

The invention belongs to the technical field of micro-plastic pollution assessment, and particularly relates to a method for assessing micro-plastic pollution level based on physiological indexes of drosophila melanogaster, which comprises the following steps: (1) collecting drosophila melanogaster in the same development stage, and selecting healthy drosophila melanogaster; (2) preparing a micro-plastic polluted culture medium; (3) putting healthy drosophila melanogaster into a micro-plastic pollution culture medium, continuously culturing for 5 days at a constant temperature, collecting the egg laying quantity of the drosophila melanogaster, and determining the reproductive toxicity caused by micro-plastic pollution under different concentrations; (4) putting healthy drosophila melanogaster into a micro-plastic polluted culture medium, continuously culturing for 20 days at a constant temperature, and measuring the behavior activity, the food intake and the in-vivo metabolic substance content of the drosophila melanogaster; and (5) performing activity correlation analysis on reproductive toxicity, behavioral activity, food intake and in-vivo metabolic substance content by using a Ttest. According to the invention, scientific theoretical support is provided for evaluating the safety problem of micro-plastic pollutants on an ecological system and human health.