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Application of cyanidin-3-O-glucoside in preparation of drugs for treating and/or preventing hormonal disorder diseases

A technology of cyanidin and glucoside, which is applied in the field of medicine, can solve the problems of unclear protection mechanism and no interference with the protection effect, and achieve the effect of promoting spermatogenesis

Inactive Publication Date: 2019-07-12
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is no relevant report on the protective effect of C3G on the interference of puberty sex hormone secretion disorder caused by Cd in the current research, and its protective mechanism is still unclear

Method used

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  • Application of cyanidin-3-O-glucoside in preparation of drugs for treating and/or preventing hormonal disorder diseases
  • Application of cyanidin-3-O-glucoside in preparation of drugs for treating and/or preventing hormonal disorder diseases
  • Application of cyanidin-3-O-glucoside in preparation of drugs for treating and/or preventing hormonal disorder diseases

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 Experimental method

[0043] 1. Protein extraction and immunoblotting

[0044] 1. Tissue protein extraction

[0045] (1) Accurately weigh animal tissue, add protein lysate at a ratio of 1:9, and homogenize in a tissue grinder at 60 Hz for 60 s until no solids are visible to the naked eye.

[0046] (2) The homogenized sample was centrifuged at 4°C and 14000 g for 10 min, and the supernatant was taken for later use.

[0047] (3) Take the supernatant, and use the BCA method to measure the protein concentration. For the determination method, see step 2.

[0048] (4) According to the obtained supernatant protein concentration, the supernatant was diluted with a protein concentration of 2.0 μg / μL. 1 / 5 of the final volume of Protein Buffer (5×) was used for dilution, and the remaining volume was made up with PBS (pH 7.4).

[0049] (5) The sample was vortexed and mixed, boiled at 100°C for 7 minutes, and stored at -20°C until use.

[0050] 2. Determination of ...

Embodiment 2

[0120] Embodiment 2 result and analysis

[0121] 1. Effect of C3G on serum sex hormones

[0122] The concentration changes of FSH, LH, DHT and INH-B in serum of mice in different groups were measured by ELISA method at different treatment time points (0, 10, 20, 30 d).

[0123] The results showed that after 20d and 30d of Cd injury treatment, the concentration of FSH in the Cd modeling group increased significantly, but after the intervention of C3G, its concentration decreased ( figure 1 A). FSH can act on supporting cells and stimulate supporting cells to produce INH-B, while INH-B can negatively feedback on the pituitary to regulate the secretion of FSH. By measuring the content of INH-B in the serum, it was shown that there was no significant difference in the expression of INH-B in each group after 20 days, and after 30 days of treatment, the content of INH-B in the Cd modeling group began to decline ( figure 1 D).

[0124] Also measure the content of the LH that pitu...

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Abstract

The invention discloses application of cyanidin-3-O-glucoside in preparation of drugs for treating and / or preventing hormonal disorder diseases. It is found that the cyanidin-3-O-glucoside can improvethe androgen secretion disorder caused by Cd exposure during puberty, and the hypothalamus-pituitary-gonad axis can be protected from injuries caused by the Cd exposure. According to C3G intervention, the synthesis and secretion of hypothalamic gonadotropin releasing hormones, the expression of pituitary and testicular sex hormone receptors and the expression of testosterone synthesis pathway-related proteins in the testis can be intervened, so that the testosterone concentration returns to a normal level, the androgen level in the inner environment of the testis is maintained accordingly, and the generation of sperms is promoted. The application provides a theoretical basis for future research on the nutrition intervention of anthocyanins with the Cd reproductive toxicity and has an important significance.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of cyanidin-3-O-glucoside in the preparation of medicines for treating and / or preventing hormone disorder diseases. Background technique [0002] Cadmium (Cadmium, Cd) is one of the common heavy metal environmental pollutants, which is mainly produced by smelting, electroplating, production of nickel-cadmium batteries, chemical fertilizers, etc. and remains in the environment. Cd can accumulate in the human body for up to 20 years through food, drinking water and air, and cause damage to multiple organs and tissues such as the nervous system and reproductive system. Cd can directly damage hypothalamic neurons through the blood-brain barrier (BBB), and can also affect the expression of proteins related to the testosterone synthesis pathway through the blood-testis barrier (BTB), and finally interfere with the hypothalamus- The pituitary-gonadal (Hypothalamus-pitu...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P5/26
CPCA61K31/7048A61P5/26
Inventor 白卫滨李旭升蒋鑫炜田灵敏孙建霞焦睿
Owner JINAN UNIVERSITY
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