193 results about "Cyanidin" patented technology

The invention relates to a method for extracting cyaniding and belongs to the technical field of extracting natural medicaments. The method adopts the process of ultrasonic-assisted enzyme method extraction, flocculation, ultrafiltration, macroporous resin adsorption, nanofiltration condensation, spray drying and high-purity cyaniding. Compared with the traditional processes, the method has the advantages of overcoming the defects in the traditional processes, effectively improving the yield and the quality of the product, greatly lowering the consumption and the loss of the organic solvents,reducing energy consumption, lowering production cost, having practicability and progress and reaching the domestic leading level.

The invention provides a purple corn beverage and a preparation method thereof. The major raw materials of the beverage are purple corn seeds and purple corn byproducts, fruit and vegetable pulp and flavorings are added to the beverage, and the beverage is treated by enzymolysis and acylation, so that cyanidin in the raw material purple corn is effectively extracted and stably stored in the beverage, thereby obtaining a purple corn beverage with rich cyanidin and unique taste.The scheme of the invention realizes the overall utilization of the purple corn plants; the cyanidin provides the function of health care, and the fruits, vegetables and flavorings not only feature the unique mouthfeel, but also boast various nutrient contents; and thus, the purple corn beverage is a novel health care beverage.

The invention relates to an anthocyanidin made from Vaccinium uliginosum L. and separation and purification methods thereof, which belong to the technology field of active component extraction and purification and functional food development. The content of anthocyanidin in Vaccinium uliginosum L. is 29.8-40%, wherein the content of malvidin accounts for 4.68-5.48% of the anthocyanidin; and antioxidant activity of the anthocyanidin is as 7.8-15 times as that of Vc. The preparation method includes pre-treating raw material, cold soaking and concentrating, purifying and concentrating, and freeze dying. The ultrasonic and microwave-assisted cold soaking method is employed for separation, and an AB-8 macroporous resin static-dynamic combined absorption purification method is employed. The invention provides an environmental friendly, high-efficiency and low-cost separation and purification technology of anthocyanidin made from Vaccinium uliginosum L., which maintains product bioactivity such as antioxidant property of product and ideal separation effect, establishes the basis for wide utilization of abundant resource of Vaccinium uliginosum L. and industrialized development promotion of a series of Vaccinium uliginosum L. health food, and embodies great economic benefit and social benefit in deep processing of the Vaccinium uliginosum L.

The process of preparing bayberry cyaniding extract includes the following steps: 1. soaking fresh bayberry pulp in 1-10 times water solution of alcohol of 50-95 wt% concentration for over 4 hr; 2. filtering to eliminate alcohol and obtain medicine liquid; 3. regulating pH of the medicine liquid to 1-4 and adsorption in macroporous resin column; washing the column until the effluent becomes no color; 5. eluting with 20-95 wt% concentration water solution of alcohol and collecting the eluted liquid; and 6. filtering to recover alcohol and vacuum drying the eluted liquid at 40-80 deg.c. The present invention also discloses the quality standard and use as food and medicine colorizing agent of the prepared bayberry cyaniding extract.

The invention provides a preparing method of purple corn cyanidin, using purple corn plant as material, first hydrolysis of material by cellulase, regulating pH value by citric acid in the hydrolysis process; then leaching by adding water and / or alcohol, or alcohol water solution in acidic environment which maintaining by citric acid leaching system; separating the leached material and collecting extracting solution; condensing the extracting solution as required to get purple corn cyanidin extracting solution; and, controlling temperature lower than 70 DEG C in whole manufacture process. Adding beta-cyclodextrin or maltodextrin, spray drying to get microcapsule product containing purple corn cyanidin. By using the method of this invention, purple corn cyanidin has high extractability, and obtained products at least contain more than six kinds of anthocyanin or derivant components thereof.

The invention discloses the preparation method of carotene, which comprises the following steps: (1) cleaning purple carrot and slicing; (2) extracting and filtering; (3) column chromatography; (4) concentrating and ultra-filtering; (5) micro-encapsulating; (6) spray-drying; and (7) sieving and mixing. The invention also discloses a carotene product, which is purple black powder with a color value equal to or larger than 50 and no peculiar carrot smell, and the carotene product is rich in cyanidin, has resistance to light and heat, and is insensitive to the pH value. Compared with the prior art, the invention has the advantage that the carotene preparation method is provided on the premise that ensures product style and internal quality stability and meets the requirement of consumers and has simple process, mild process condition, short preparation cycle, less equipment investment, recycle use of waste water and reuse of waste as feed additives, and the method is suitable for continuous and large-scale industrial production. The carotene of purple carrot has the advantages of low cost, stable quality and high reliability.

The natural flower health drink, including flower wine, flower honey and flower tea, is prepared with over 100 kinds of wild flowers and through stoving, gamma ray irradiation and soaking in wine, honey or drinking water. The selected wild flowers contain amino acid, natural enzyme, vitamins, cyanidin, aromatic oil and other matters essential for human life activity, some matters with antisenility, antifatigue, anti-cancer and antibacterial functions and trace elements needed by human body.

The invention discloses a preparation method of polyphenol of chestnut shells. The preparation method comprises the following steps of digestion, filtration and condensation, enrichment / separation, condensation and drying to obtain the polyphenol of brownish red chestnut shells. The preparation method has the advantages of high content and good quality of polyphenol with the content of oligomeric proantho cyanidins reaching more than 50 percent, simple production technology, low cost and no pollution, and is applicable to commercial production. The preparation method maximizes the utilization of the waste chestnut shell which accounts for 20 percent of a chestnut, thus enhancing the added value of chestnuts and simultaneously creating a new resource of antioxidant of polyphenol from natural plants for the food and pharmaceuticals industries.

The blueberry tea beverage as one kind of improved beverage is prepared with blueberry leaf, fruit material suspending agent and pure water. The beneficial effects of the blueberry tea beverage include: 1. rich vitamin C, flavone, SOD and cyanidin contained in blueberry leaf resulting in natural health functions of eliminating fatigue, resisting senility, eliminating free radical from inside human body and protecting eyesight; 2. great amount of organic acid contained in blueberry leaf resulting in boiled solution pH value of 2.5-3.5, no need of adding other organic acid and excellent taste; and 3. the orange color of the boiled blueberry leaf solution resulting in good look.

The invention relates to the technical field of extraction of effective parts of plants, in particular to an extraction and separation method of flavone and cyanidin from elderberry fruits. The invention aims to overcome the problems of complicated raw material treatment, low recovery and content of target constituents of products, and poor product color in the prior art. The provided technical proposal is as follows: the extraction and separation method of the flavone and cyanidin from the elderberry fruits comprises the following steps: first, extraction: crushing the raw materials, adding to a container for evenly stirring, dipping and filtering to obtain clear leach solution of the elderberry fruits for use; second, performing adsorption and elution by a macroporous resin adsorption column to obtain a condensed extract; and third, drying the extract to obtain a finished product. Compared with the prior art, the method has the advantages of 1. simple raw material treatment, 2. high recovery and content of target constituents of the product, 3. good color (mauve), and 4. simple process.

The invention provides a blueberry leaf health care beverage. The beverage comprises the following materials in weight portion: 30 to 50 portions of blueberry leaf, 10 to 30 portions of blueberry fruit, 5 to 10 portions of seabuckthorn fruit, 10 to 20 portions of rose hip and 10 to 20 portions of cordyceps militaris, and active ingredients of the materials are extracted by microwave technique to prepare the beverage. In all materials, vitamin C is abundantly contained, particularly, cyanidin composition and flavonoid contained in the blueberry leaf can delay ageing, improve the immunity of human body and obviously improve eyesight of people and has a plurality of physiological activities such as blood sugar and blood fat adjustment, and the like; the cyanidin composition is a natural bacteriostat, colorants and bacteriostats are not necessary to be added, and flavoring agents such as citric acid are not necessary to be added to regulate the acidity, which is more helpful to the health of human body. By the verification of experiments and the experience of people, the health care beverage is easily absorbed by human body and taken conveniently, and can obviously improve the eyestrain of human body; moreover, the health care beverage also has the characteristics of unique formula, reasonable composition, simple process, body building-up, remarkable function and the like.

The invention relates to a treatment method for enhancing stability of blueberry cyanidin. The treatment method comprises the following steps: extracting with a 0.5% trifluoroacetic acid (TFA) methanol solution, and sequentially purifying through an ion exchange resin column Amberlite XAD-7 and a Sephadex column Sephadex LH-20 to obtain a blueberry cyanidin refined substance; carrying out nutgall acylation reaction on the blueberry cyanidin refined substance and prepared triacetyl nutgall acyl chloride to introduce galloyl group with ortho-triphenolhydroxy group into the molecular structure, thereby obtaining the modified product nutgall acylated cyanidin. When high-performance liquid chromatography is used for determining the gallic acid amount generated after hydrolyzing the modified product, the nutgall acylation degree is 55-63%. The experiment proves that the stability of the modified product is obviously enhanced. The method for treating blueberry cyanidin is simple to operate, and has the advantages low cost, low pollution and high acylation degree. The molecular modification can enhance the primary characteristics of the blueberry cyanidin, so that the effects are enhanced; and multiple functional groups are introduced to endow the blueberry cyanidin with new physiological activity, thereby widening the application range.

The invention provides a method for separating cyanidin in nitraria sibirica pall through high-speed counter-current chromatography. With utilization of the high-speed counter-current chromatography and adopting of a reasonable solvent system, the high-purity cyanidin is obtained from nitraria sibirica pall; the method requires no use of a solid-phase carrier, has no irreversible adsorption, and separates and prepares cyanidin anthocyanin with no loss of samples, no pollution, high efficiency, speediness and low cost. The method has the advantages of simple process, convenient operation, safety, and good reproducibility, and can be used for industrialized production.

The strawberry freeze drying process includes: A. compounding water solution of beta-cyclodextrin of 0.08-0.12 wt% concentration; B. mixing cyanidin solution of 0.001-0.0013 wt% concentration and the beta-cyclodextrin solution of 0.08-0.12 wt% concentration; and C. setting the mixed solution and fresh strawberry in the weight ratio of 2.5-3 inside some vacuum container and vacuum pumping to 250-350 mmHg for 3-10 min. The said process can produce freeze dried strawberry with less nutritious component loss, color change and taste change and the freeze dried strawberry may be re-combined with water to restore the fresh strawberry. The said technology of the present invention may be used also for apple, pear, peach, almond, tomato and cucumber.

The present invention relates to a natural red anthocyanidin cosmetic. It is prepared by using (by wt%) 0.5-30% of natural red anthocyanidin and 70-99.55% of cosmetic base material though a conventional process. The color of natural red anthocyanidin can be regulated according to its pH value which is 4-9.

To prevent or alleviate various pathologies caused by lifestyle-related diseases, etc., the present invention provides a substance having an effect of promoting the expression and elevating the expression level of adiponectin, and applications thereof. An adiponectin expression promoting agent containing, as an active ingredient, at least one cyanidin compound selected from the group consisting of cyanidin and cyanidin glycosides can be applied to pharmaceutical or food compositions for use in applications in which the pharmacological effects of adiponectin are utilized.

The invention discloses a method for preparing a liquor from five coarse cereals, which uses the following raw materials in parts by weight: 1-10 parts of black corn, 1-6 parts of triticale, 0.5-1 part of black bean, 1-6 parts of black glutinous rice and 1-7 parts of sorghum. The method comprises the following specific preparation steps: soaking, performing primary raw material liquid-state fermentation, distilling, performing secondary mature material liquid-state fermentation and squeezing. The process method is unique, and innovatively combines the liquor process and the wine process. The brewed liquor is brilliant red and transparent in color and mellow in mouthfeel, is rich in cyanidin and selenium and has a high nutritive value, thus being a novel liquor combining the advantages of the liquor and the wine.

The invention relates to a method for extracting cyanidin from black bean peel, which comprises the following steps: A) after cleaning black bean peel, adding water and ethanol into the black bean peel to carry out extraction, thereby obtaining an extracting solution; B) carrying out centrifugal separation on the extracting solution to obtain a supernatant, carrying out trash extraction on the supernatant with a ceramic membrane to obtain a ceramic membrane permeation solution; C) concentrating the ceramic membrane permeation solution with an organic nanofiltration membrane to obtain a concentrated solution, and eluting the resin to obtain an eluate; and D) carrying out spray drying on the eluate to obtain the cyanidin. By adopting the centrifugal machine separation, membrane separation and resin integration technology, the invention can effectively lower the production cost, shorten the extraction separation time and enhance the product yield and product purity.

The invention relates to a method for extracting proantho cyanidins from litchi peels and belongs to the technical field of active substance extraction from agricultural products. The method comprises the steps of raw material treatment, ultrasonic pretreatment, extraction, purification, drying and the like. The method utilizes the ultrasonic extraction technology, together with ethyl alcohol, to extract proantho cyanidins from litchi peels to achieve the purposes of improving the proantho cyanidins extraction ratio, shortening the extraction time, reducing the extraction temperature and maintaining the activity of extracted proantho cyanidins, and the extraction process is efficient, pollution-free and low in cost.

The invention provides a black rice extract. The mass content of cyaniding in the black rice extract is 0.5-50%. The invention further provides a method for preparing the black rice extract and the application of the black rice extract to skin care products. The content of active ingredients in the black rice extract is high. Besides whitening, the black rice extract can also well resist aging and keep skin elastic by promoting collagen secretion; the black rice extract can remove free radicals in human cells so as to resist aging, and can be added to skin care products as a composite additive.

The invention relates to application of oligomeric proantho cyanidins in preparing products for preventing, diagnosing, detecting, protecting, treating and studying vascular dementia and directly related diseases thereof, in particular to new application in medicament and health-care products. Experiments prove that oligomeric proantho cyanidins can improve the study memory ability of a heart and brain ischemia-reperfusion mouse and a permanent bilateral common carotid arteries rat, inhibit lipid peroxidation in brains of the heart and brain ischemia-reperfusion mouse and the permanent bilateral common carotid arteries rat, and improve the superoxide dismutase enzyme activity of the heart and brain ischemia-reperfusion mouse and the permanent bilateral common carotid arteries rat. The oligomeric proantho cyanidins have strong action, clear effect, rich resource, simple preparation process, high yield, stable property and safe use, in particular one object with multiple functions and wide application range, and can play roles furthest. Disease rate of dementia rises gradually, after popularization, the application of oligomeric proantho cyanidins can generate significant social benefit and economic benefit in short time.

The invention discloses a protection effect of cyanidin-3-glucoside (C3G) for UVB (ultraviolet B)-induced cell injury. The protection effect particularly includes that generation of ROS (reactive oxygen species) of HaCaT cells due to UVB can be reduced by the C3G, accordingly, antioxidant effects can be realized, DNA (deoxyribonucleic acid) can be protected against injury, the survival rate of the HaCaT cells can be increased after the UVB irradiates on the HaCaT cells, and apoptosis reducing effects and the like can be realized. The protection effect has the advantages that the oxidation injury of the selected representative HaCaT cells which are cultivated in vitro is induced by means of UVB irradiation, light injury models of epidermal cells due to ultraviolet rays are imitated, then the C3G is used as a light injury protector, effects and relevant channels of the C3G for relieving UVB injury of the HaCaT cells are evaluated, the protection effect, action mechanisms and the safety of the C3G for light injury of skins are investigated, and accordingly theoretical foundation is laid for clinically effectively preventing and treating light injury of the skins and studying ultraviolet radiation injury protection action mechanisms of natural plant antioxidants.

The invention discloses a gene PpRd for regulating fruit flesh cyanin synthesis and application thereof, relating to plant gene engineering and biotechnology. The gene PpRd nucleic acid sequence is disclosed as SEQ ID NO.1, and the protein sequence coded by the gene PpRd is disclosed as SEQ ID NO.2. The application of the PpRd gene is implemented by (1) designing a molecular marker WPS23 according to the sequence of the PpRd gene, and applying the molecular marker WPS23 in marker auxiliary screening in breeding; and (2) application of expression protein sequence and gene functions: detecting and screening cyanidin content related phenotype according to the expression level of the gene, or enhancing or lowering the gene expression level by genetic transformation to obtain the expected flesh color character. The invention obtains a key gene for cyanin accumulation. When the PpRd gene is used for marker auxiliary selection to instruct hybridization breeding of peaches, the screening can be performed in advance, thereby saving the breeding time and enhancing the breeding efficiency.

The invention discloses a method for extracting cyanidin from purple yam peel, which comprises the following steps: (1) selecting purple yam peel, cleaning to remove impurities, pulverizing into powder, drying in a 50-DEG C drying box, passing through an 80-100-mesh screen, and keeping in a dark place for later use; (2) accurately weighing the purple yam peel powder, adding an ethanol extracting agent, leaching in a thermostatic water bath, filtering and diluting to a specific volume; and (3) concentrating the extracting solution by evaporation to obtain a paste, and drying to obtain the cyanidin. According to the influence of the extraction conditions on the cyanidin in the purple yam peel, the optimal extraction time, extraction temperature, material / liquid ratio and extracting agent concentration are adopted to ensure the higher extraction rate and purity of the cyanidin. The method is simple to operate and low in cost, avoids waste of resources, and is suitable for industrial production.

The invention discloses a pitaya juice and a processing method thereof, and belongs to the technical field of food processing, wherein the pitaya juice comprises the following raw materials by weight: 1000-1200 parts of pitaya flesh, 50-85 parts of pitaya pericarp, 400-500 parts of kiwifruit, 300-400 parts of cucurbita pepo l, 45-75 parts of a color protection agent, 35-65 parts of a flavoring agent, 30-45 parts of a preservative, and 15-25 parts of citric acid. The processing method comprises: 1) raw material treatment; 2) astringent taste removing treatment; 3) mixing and juice preparation; 4) seasoning; and 5) homogenization and degassing. According to the present invention, the pitaya flesh adding amount can be reduced while the cyanidin content in the pitaya juice can be increased, and the nutrients in the pitaya juice can be increased; and the pitaya juice is suitable for general people, and is further suitable for diabetes patients.

Methods for preparing black soybean hull extracts and uses thereof. The methods can comprise: (1) extracting crushed black soybean hull with aqueous solution containing enzyme and filtering the extracted liquid; (2) ultrafiltration of the extract liquid with an ultrafiltration membrane; (3) absorbing the ultrafiltration liquid with an absorption resin or an ion exchange resin, and elution with ethanol to obtain purified extract liquid; (4) concentrating the purified extract liquid under vacuum; (5) spray drying the concentrated liquid. The extract can contain: 10-45% of cyanidin, 10-25% of catechin, and 40-80% of OPC, and with a total polyphenol content as high as 70-100%. The extract can be used for preparing drugs or food containing antioxidants, or used for improving metabolic syndrome or eyesight.

The invention discloses application of brown cyanidin in preparing a medicament for treating high blood fat diseases, in particular a medicament for treating high blood fat diseases of high triglyceride and high serum low-density protein types. The applicant discovers that the brown cyanidin has remarkable curative effect. Meanwhile, the invention also provides a preparation for treating the diseases.

The invention discloses a method for extracting cyanidin from roselle. The method comprises the following steps: cleaning roselle, drying, pulverizing, and screening with a 100-mesh screen; adding the roselle particles and deionized water into an extractor according to the material / liquid ratio of 1:8-1:12, and extracting twice to obtain an extracting solution; filtering the extracting solution with a centrifuge, and removing solid impurities to obtain a filtrate; carrying out ultrafiltration through a hollow fiber membrane; carrying out nanofiltration through a nanofiltration membrane; finally filtering and concentrating through a disk-type tubular organic membrane to obtain an extract; and carrying out freeze-drying on the extract to obtain the cyanidin product. The method obviously increases the yield of the cyanidin, enhances the purity of the cyanidin product, greatly lowers the conversion of cyanidin in the industrial production process, has the advantages of energy saving and consumption reduction, reduces the environmental pollution, and is beneficial to the proceeding of the factory production and the reduction of the production cost.

The invention relates to a method for extracting grape seed oil from grape seeds, in particular to a method for extracting grape seed oil containing oligomeric proantho cyanidins (OPC), which is characterized by comprising the following steps: a, crushing grape seeds by using a plant tissue crushing extraction method; b, carrying out supercritical CO2 leaching on the crushed grape seeds to obtainthe grape seed oil containing OPCs; and c, standing to separate out ethanol. By using the extraction process provided by the invention, the extraction rate of the grape seed oil can be up to 98.72%, the content of OPCs in the oil is 2.14mg / mL, and the scavenging rate IC50 value of diphenylpicrylhydrazyl (DPPH) free radicals is 11.45mg / mL and is lower than the IC50 value (12.80mg / mL) of the grape seed oil not containing OPCs, thereby proving that the antioxidant activity is improved.

The invention relates to preparation of cyanidin, particularly an extraction method of date peel cyanidin, which comprises the following steps: drying date peel, pulverizing, and screening with a 20-60-mesh sieve; adding the date peel powder and pure water into a vessel according to a solid-liquid mass ratio of 1:(15-25); adding 1-3% special composite enzyme for dates into the vessel until the pH value of the solution is regulated to 5-7, and oscillating the solution for 17-19 hours at the constant temperature of 40-60 DEG C; adding a 2-4% sodium hydroxide solution into the enzymolyzed solution, wherein the volume ratio of the added sodium hydroxide solution to the enzymolyzed solution is (0.4-0.6):1; centrifuging the sodium-hydroxide-added solution 2-4 times, filtering out impurities, and collecting the supernatant; and adding 0.5-2.5% vitamin A or vitamin B6 into an ethanol-added solution, wherein the volume ratio of the vitamin A or vitamin B6 to the ethanol-added solution is 1:(8-16). The invention solves the problems of poor stability and low extraction rate of natural cyanidin, and has the advantages of low production cost, simple technique, strong operability and huge economic benefit.