72 results about "Collagen secretion" patented technology

A method for treating cardiac fibrosis resulting from injury of a mammalian heart is described comprising the step of contacting a therapeutically effective amount of relaxin and / or an LGR7 activating agent with cardiac cells following the injury in an amount sufficient to reduce the fibrosis. Also described are methods for protecting the heart following an ischemic event, inhibiting the proliferation of activated fibroblasts and antagonising collagen secretion or deposition in a mammalian heart.

The invention provides compound cockroach glucosides A to C and a drug composition thereof and their application in preparation of pharmaceuticals and health-care products for treating ulcers and burns and scalds. Ulcers and burns are fairly common, and American cockroaches clinically used for various ulcers and burns have a good effect. Three dihydrogen isocoumarin glucosides are separated and identified and are named as the cockroach glucosides A, B and C, and all the cockroach glucosides can promote dermal fibroblast collagen secretion of human and improve the treatment effect on the collagen secretion lack diseases, such as all kinds of ulcers, burns and scalds.

The invention provides a plukenetia volubilis L. stem leaf extract capable of delaying senescence as well as a preparation method and an application of the extract in the aspects of cosmetics, health food and the like. The invention also relates to a cosmetic composition which is used for resisting oxidation, preventing skin aging and removing wrinkles and contains the plukenetia volubilis L. stem leaf extract as an active ingredient. The plukenetia volubilis L. stem leaf extract has inhibition activity on elastinase, collagenase and hyaluronidase and has significant effects in collagen secretion promoting experiments. The usage amount of the extract in health food or anti-aging cosmetics can be 0.01wt%-100wt%.

The invention relates to an anti-aging skin care product, and in particular discloses a stem cell essence and a preparation method thereof. The stem cell essence is prepared by mixing the following raw materials in percentage by weight: 28-32% of a stem cell extract, 3-7% of glycerol, 0.2-0.8% of lactic acid, 0.7-1.3% of trehalose, 0.2-0.5% of vitamin A acetate, 0.1-0.3% of vitamin E acetate, 0.1-0.3% of 4',6,7-trihydroxyisoflavone, 0.5-0.7% of titanium dioxide, 0.01-0.02% of xanthan gum, 0.02-0.03% of triethanolamine, 2-4% of isopropyl myristate, 0.8-1% of propylene glycol, 0.02-0.07% of sodium hyaluronate, 0.8-1.2% of an antiseptic and the balance of de-ionized water, wherein the stem cell extract is an extract of sheep umbilical cord blood stem cells. The stem cell essence provided by the invention, after being absorbed by the epidermis, can penetrate to the corium layer and the subcutaneous tissue, so as to comprehensively activate the differentiation of stem cells in skin and rapidly replace old cells, so that collagen secretion is enhanced and skin elasticity is improved; the stem cell essence can be used for promoting the synthesis activation of protein and enhancing the softness of the skin; and the stem cell essence can be also used for stimulating the muscular layer to generate fibroblast and skin protein so as to enhance skin toughness and elasticity.

The invention provides a black rice extract. The mass content of cyaniding in the black rice extract is 0.5-50%. The invention further provides a method for preparing the black rice extract and the application of the black rice extract to skin care products. The content of active ingredients in the black rice extract is high. Besides whitening, the black rice extract can also well resist aging and keep skin elastic by promoting collagen secretion; the black rice extract can remove free radicals in human cells so as to resist aging, and can be added to skin care products as a composite additive.

The invention relates to microneedle paster for replenishing water and dispelling wrinkles and a preparation method of the microneedle paster, and relates to the field of skin-care products. The microneedle paster comprises a base and a microneedle, wherein a plurality of needles are uniformly distributed on the base; the base and the needles are made from main body support components, and functional components are dispersed in the main body support components; and the main body support components are hyaluronic acid, and the functional components are bioactive peptide P-WH. The microneedle paster disclosed by the invention can effectively penetrate through stratum corneum to dissolve by tissue liquid of a cuticular layer and epidermis, P-WH is released to cuticle and dermal tissue to diffuse so as to stimulate secretion of skin cell collagen, and the effects of preventing and repairing skin ageing and rough skin, replenishing water and resisting ageing can be achieved.

The invention provides ultrashort peptide for promoting collagen secretion and an application of ultrashort peptide, and belongs to the technical field of biomedicine. The ultrashort peptide containsat least one of ultrashort peptide Mapin-WH, Tinin-WH and Orin-G-OT, has the capability of promoting collagen synthesis, and has the characteristics of extremely high oxidation resistance and anti-aging activity, small molecular weight, simple structure, extremely low cytotoxicity, no hemolytic activity, simple preparation method and the like. The ultrashort peptide is used for preparing medicinesfor repair and regeneration of damaged epidermis such as burns, scalds and skin ulcers and used for replacing EGF in the fields of new raw materials for anti-aging and anti-oxidation beauty skin careproducts for repairing skin and stimulating skin collagen hyperplasia, and functions of the ultrashort peptide is probability not limited to the fields. The ultrashort peptide has small molecular weight and simple structure, is quite low in cost with the adoption of chemical synthesis and has outstanding beneficial characteristics as compared with the EGF costing nearly 2 million US dollars per gram.

The invention discloses a method for detecting and evaluating in-vitro cell morphology and osteogenic function of the surface of a bone substitute implant, and belongs to the technical field of biomaterial detection. The method is characterized by comprising the following steps: taking the bone substitute implant to be detected and evaluated, firstly, observing the cell morphology, cytoskeleton morphology and skeleton protein distribution condition, then detecting cell activity, cell adhesion on the surface of the material and alkaline phosphatase activity in cells, then detecting collagen secretion and the external parts of the cells, and remembering the mineralization degree, and finally, determining the cell morphology as well as the expression condition of framework related genes and osteogenesis related genes. According to the method, biological reference is better provided for optimal design of the surface of the bone substitute implant, and on the basis of the prior art, lots of detecting methods are added, and part detecting methods are improved, so that the method is comprehensive and systematical, and passes the cytology and molecule biology cross comprehensive evaluation, and important guiding significances are provided for optimal research of the surface of the bone substitute implant.

The invention belongs to the technical field of biological pharmacy, and particularly relates to application of active peptide and a mesenchymal stem cell exosome for improving skin physiological characteristics in drugs or cosmetics. Amino acid replacement is performed on the basis of original active peptide, and other conventional amino acids and unusual amino acids are introduced, so that the antioxidant capacity, collagen secretion promoting capacity and cell proliferation promoting capacity are improved. The effect of combined use between the amino acids and the human umbilical cord mesenchymal stem cell exosome is more obvious, skin injuries caused by ultraviolet rays can be effectively prevented and treated, and the repair process after the injuries is promoted.

The invention belongs to the field of biology, and particularly relates to a skincare stem cell activin and a preparation method and application thereof. The preparation method of the skincare stem cell activin includes the steps of S1, culturing stem cells in vitro; S2, collecting the stem cells, performing cell lysis to obtain cell lysate, subjecting the cell lysate to low-speed centrifuging, removing cell debris, and freezing to preserve supernate to obtain the stem cell activin. Compared with general chemical skincare products, the skincare stem-cell activin has the advantages that under action of the cell signal regulation mechanism and the synergistic effect of various growth factors, nutrition metabolism of epithelial cells can be improved, skin protection can be achieved, skin injury caused by various reasons can be prevented and cured, skin collagenocyte growth can be accelerated, collagen secretion of skin can be accelerated, and further, wrinkle resistance and ageing resistance can be achieved. Therefore, the skincare stem cell activin has good application prospect in fields of medicines, healthcare and beautifying.

The invention discloses applications of pseudolaric acid B in preparation of anti-pulmonary-fibrosis medicines. The structure of the pseudolaric acid B is shown as a formula (I). Experiments prove that the pseudolaric acid B (PLAB) obviously relieves the degree of alveolar catarrh and pulmonary fibrosis of mice in a pulmonary fibrosis model built by utilizing bleomycin, and inhibits collagen secretion and deposition in fibrotic lung tissues, thus preventing and treating pulmonary fibrosis.

The invention discloses a reversible collagen stimulating filling agent and a preparation method thereof. By utilizing temperature-sensitive and ion response characteristics of hydroxybutyl chitosan,formation, separation and curing of microspheres are promoted, and the degradation time is prolonged; by utilizing positive and negative charge attraction between hyaluronic acid carrier gel and hydroxybutyl chitosan microspheres, uniform and stable dispersion of the microspheres can be promoted, and agglomeration of the microspheres can be effectively prevented. The preparation process is simplein steps, environmentally friendly in technical route, low in cost and favorable for industrialization. The prepared reversible collagen stimulating filling agent has long degradation period under normal physiological conditions, can effectively stimulate collagen secretion for a long time, particularly has an effective emergency degradation mechanism, and can be rapidly degraded by taking measures during overcorrection or misoperation, and risk of product injection is reduced. The reversible collagen stimulating filling agent provided by the invention has effectiveness and safety, and has excellent application prospects in the soft tissue filling field, particularly medical cosmetology filling.

The invention discloses a membranous matrix (cytoskeleton) which is prepared by mixing collagen and chitosan and then drying the mixture. The preparation process is simple. The collagen component is collagen I extracted from the rat tails, accounting for 90% of the weight of the mixture. The chitosan component is 75-95% of deacetylated chitosan, accounting for 10% of the weight of the mixture. The invention adopts the composite membrane as the cytoskeleton for constructing the tissue-engineered tympanic membrane and finds that the composite membrane is suitable for grow and proliferation of epithelial cell / keratinocyte and fibroblast of the tympanic membrane and can maintain good collagen secretion function of the fibroblast. The cytoskeleton further lays the foundation for constructing the tissue-engineered tympanic membrane.

The invention discloses a truncated growth differentiation factor 11 (GDF11) with biological activity and a preparation method thereof and belongs to the technical field of biological medicines. The truncated growth differentiation factor 11 comprises 95 amino acids, wherein the amino acid sequence is as shown in SEQ ID NO.1 The biological activity of the obtained truncated growth differentiation factor 11 is researched, and the result shows that the truncated GDF11 is capable of stimulating collagen secretion of fibroblasts and has the activity of promoting phosphorylation of cardiac fibroblasts Smad2 / 3 and activating a passage of the cardiac fibroblasts Smad2 / 3. Therefore, the truncated growth differentiation factor 11 has a wide application prospect in treatment or prevention of irreversible senile diseases such as a diastolic heart failure and alzheimer disease.

The invention relates to the technical field of biological medical adhesives, and particularly relates to a chitosan cicatrix biological medical adhesive and a preparation method thereof. Each liter of the chitosan cicatrix biological medical adhesive comprises the following raw materials: 5-30 grams of modified chitosan, 30-50 milliliters of glycerol, 0.5-2.5 grams of herba menthae, 0.1-10 grams of lactic acid, 0.5-2.5 grams of ginger extracting solutions, 3-35 grams of viscosity regulating agents and the balance of purified water. The chitosan cicatrix biological medical adhesive disclosed by the invention is complementary in raw material, can effectively reduce the pruritus, relieve the pain, fade the cicatrix color, ensures that the cicatrix is gradually reduced in area and become smooth, is suitable for inhibiting, alleviating and restoring the cicatrice caused by reasons, such as various burns and scalds, traumata, scratches, operations and acne, accelerates the healing of a wound surface, restores the surface cracks of the cicatrice, inhibits the secretion of collagen inside keloid skin, reduces the pruritus, relieves the pain and fades the cicatrix color.

The invention provides a medicine composition and uses thereof. The medicine composition comprises: mesenchymal stem cells and soft tissue filler, wherein the soft tissue filler comprises at least one of collagen, hyaluronic acid, calcium hydroxyapatite, polylactic acid, fat and platelet rich plasma, and the mesenchymal stem cells are in contact with collagen stimulating factors in advance. The medicine composition is used for improving cell activity such as fibroblast activity. Compared with the prior art, the medicine composition has the advantages of low immunogenicity, quick effectiveness, good naturalness, good treatment effect, more permanent effect and low risk. The medicine composition can be effectively used for removing wrinkles, resisting aging and scars, and repairing wounds.

The invention discloses an application of bone morphogenetic protein-4 in screening drugs for resisting cardiac hypertrophy, heart failure or cardiac fibrosis and belongs to the field of biomedicine. Screened drug types comprise: bone morphogenetic protein-4 formation inhibitor, bone morphogenetic protein-4 antagonistic, bone morphogenetic protein-4 receptor antagonists and bone morphogenetic protein-4 downstream signal antagonistic. The invention also relates to an application of the drugs in preparation of medicines for resisting cardiac hypertrophy, heart failure and arrhythmia. Researches have found that bone morphogenetic protein-4 can induce myocardial hypertrophy, apoptosis and cardiac fibroblast collagen secretion increase, lead to occurrence of cardiac hypertrophy, heart failure and arrhythmia. However, drugs for antagonism of bone morphogenetic protein-4 and its cell signaling pathway can be used to inhibit occurrence of cardiac hypertrophy, inhibit cardiac fibrosis and minimize occurrence of cardiac cell death and arrhythmia. The invention provides a theoretical basis for realizing high-flux medicine screening, and is of practical guiding significance.

The invention belongs to the technical field of medicine and biology, and discloses application of lysophosphatidic acid. Lysophosphatidic acid can be used for regulating expression of inflammatory factors such as IL-1 and / or IL-6, and inflammatory responses are alleviated; meanwhile, collagen secretion by fibroblasts and epidermis proliferation and regeneration can be promoted. Therefore, the lysophosphatidic acid can repair wounds and promote wound healing and has a good application prospect.

The invention provides collagen secretion promotion ultrashort peptides and application thereof, and belongs to the technical field of biomedicine. The two kinds of collagen secretion promotion ultrashort peptides Magin-FL and Purin-WH3 have the capability of promoting the collagen synthesis; in addition, the characteristics of ultra-high oxidization resistance, aging-resistant activity, no cell toxicity, no hemolysis activity, simple preparation method and the like are realized, so that the collagen secretion promotion ultrashort peptides are used for preparing medicine for treating injured skin restoration and regeneration such as medicine for treating burnt injury, scald injury and skin ulceration, and can replace EGF to be used in the fields of anti-aging and anti-oxidization beatification skin care product new raw materials for skin restoration and skin collagen proliferation simulation and the like. In addition, the effects are possibly not limited to the field. The ultrashort peptides Magin-FL and Purin-WH3 have small molecular weight and simple structure; the used chemical synthesis method has very low cost; the ultrashort peptides Magin-FL and Purin-WH3 have the obvious beneficial characteristics through being compared with EFG with the sale price being nearly 2 million U.S dollars per gram.

The invention relates to the technical field of biology, in particular to a preparation method of a biological preparation for skin damage repair. The preparation method comprises the steps that P3 substituted adipose derived stem cells are taken to be recovered and added into a DMEM (dulbecco's modified eagle medium) high glucose complete medium containing 10-12% of FBS (fetal bovine serum) to becultured till the cell confluence rate is as high as 80-90%, and supernatant is collected; the P3 substituted adipose derived stem cells are washed and added into a DMEM / F12 basal culture medium to be cultured for 24 hours, and supernatant is collected; the cell culture after the supernatant is collected is taken, the culture medium is replaced, culture is conducted continuously, the culture liquid is collected, and the steps are repeated for 1-2 times; the supernatants are combined, and the biological preparation is obtained. The biological preparation prepared by adopting the preparation method provided by the invention can outstandingly promote skin fibroblast proliferation and collagen secretion, and has the good effect of promoting skin damage repair.

The invention belongs to the field of biopharmaceutical research, and in particular relates to application of anti-inflammatory and analgesic bolus and a preparation thereof in preparation of a medicine for promoting refractory wound healing. According to the application of the anti-inflammatory and analgesic bolus and the preparation thereof in preparation of the medicine for promoting refractorywound healing in the invention, due to animal experiments and immunohistological experiments, researches find that the anti-inflammatory and analgesic bolus has the obvious promoting effect on refractory wound healing; and further researches find that the promoting effect of the anti-inflammatory and analgesic bolus on refractory wound healing is mainly related to effects of reducing abnormal senescence of fibroblasts, resisting oxidization, promoting fibroblast proliferation induced by oxidative damage, promoting cell adhesion, reducing apoptosis of fibroblasts, promoting collagen secretion,etc.

The invention provides a collagen secretion promotion ultrashort peptide and application thereof, and belongs to the technical field of biomedicine. The ultrashort peptide comprises at least one kind of materials from ultrashort peptides Magin-WH, Tinin-WH and Orin-G-OT, has the capability of promoting the collagen synthesis, and has the characteristics of very strong oxidization resistance, anti-aging performance, small molecular weight, simple structure, very low cell toxicity, no hemolytic activity, simple preparation method and the like. The ultrashort peptideis used for preparing medicine for treating injured skin restoration and regeneration, such as medicine for treating burnt injury, scald injury and skin ulceration, and can replace EGF to be used in the fields of anti-aging and anti-oxidization beatification skin care product new raw materials for skin restoration and skin collagen proliferation simulation and the like. The ultrashort peptide has small molecular weight and simple structure; the used chemical synthesis method has very low cost; the ultrashort peptide has the obvious beneficial characteristics through being compared with EFG with the sale price being nearly 2 million U.S dollars per gram.

The invention provides a natural product compound 1 cis-3-(3,4-Dimethoxyphenyl)-4-[(E)-2,4,5-trimehoxystyryl]cyclohex-1-ene derived from Zingiber purpureum Rosc., application thereof in anti-aging skin care products and a preparation method thereof. The chemical constituents of an ethanol extract of Zingiber purpureum Rosc. are chemically separated to obtain the monomeric compound, and the activity of the monomeric compound for promoting collagen secretion of human dermal fibroblasts-adult (HDFa) is evaluated and analyzed. The compound 1 has the half effective concentration EC50 (mug / mL) of 13.906 plus or minus 1.562, shows no cytotoxicity to normal HDFa cells, and thus has application value in skin care products.

The invention belongs to the technical field of health-care foods and particularly relates to a gugua polypeptide composition. The gugua polypeptide composition comprises melon seed peptide and bone marrow peptide. The invention also provides application of the gugua polypeptide composition in preparing medicines and health-care foods which have the efficacy of improving immunity, promoting woundhealing as well as preventing and treating diseases caused by free radical damage. The gugua polypeptide composition provided by the invention has the effects of effectively promoting the wound healing, improving the immunity, eliminating excess free radicals in the body, realizing an anti-aging effect, promoting calcium absorption and collagen secretion, reducing morning stiffness, increasing joint flexibility, reducing joint pain and swelling, improving diseases such as osteoporosis, fracture, rheumatism, rheumatoid, scapulohumeral periarthritis and lumbar disc herniation, improving the digestive system, promoting gastrointestinal peristalsis and digestive enzyme secretion, treating chronic gastrointestinal disease, improving pancreatic function, promoting automatic insulin secretion ofthe body and reducing insulin resistance, thereby achieving the aims of decreasing blood sugar and preventing complications.

The invention relates to a preparation method and application of a bionic nano-drug for targeting hepatic stellate cells, which can effectively solve the problem that the synthesis is relatively tedious because the surface of a nano-drug is modified with a targeting ligand by a chemical modification method in the conventional nano-carrier for targeting hepatic stellate cells. According to the technical scheme for solving the problems, by using the homologous targeting function of the hepatic stellate cells, the hydrophobic small molecule drug BAY 11-7082 for inducing the apoptosis of the hepatic stellate cells (HSCs) is wrapped, and the nano-drug with the hepatic stellate cell targeting is obtained through the ultrasonic co-extrusion method, and the bionic nano-drug can induce the apoptosis of the hepatic stellate cells, reduce the collagen secretion, and can achieve the anti-hepatic fibrosis purpose by relieving inflammation microenvironment; and the preparation method provided by the invention is simple and convenient, has good biocompatibility, and is an innovation of bionic nano-drugs.

The invention relates to a facial rejuvenation beauty method based on dermal stem cells. According to the method, nutrient solution is introduced into a dermis layer, the microenvironment for survivalof the dermal stem cells is adjusted, the dermal stem cells are promoted to express related growth factors, fibroblasts are activated to stimulate collagen secretion, and proliferation of the fibroblasts is promoted, so that collagen and elastic fibers are synthesized; secretion of the collagen and elastin tends to be normal or increased, skin elasticity is enhanced, relaxation state is improved,wrinkle formation is reduced or even improved, the aging state of facial skin is improved from the root, and skin cells are rejuvenated to achieve facial skin rejuvenation.

The invention provides micromolecular sodium hyaluronate and a micromolecular sodium hyaluronate composition for improving skin aging and sensitivity and application of the micromolecular sodium hyaluronate and the micromolecular sodium hyaluronate composition, and belongs to the technical field of health food. The micromolecular sodium hyaluronate with the average molecular weight of 80KDa-800KDa has the effect of remarkably improving the water content of the skin, and the collagen peptide and the micromolecular sodium hyaluronate are micromolecules and can be easily absorbed by a human body. The composition composed of collagen peptide hydrolysate, micromolecular sodium hyaluronate hydrolysate, elastin, cubilose powder, mangosteen powder, salmon nose cartilage powder, purple chrysanthemum flower powder, astaxanthin, tremella polysaccharide, vitamin C and concentrated fruit juice can improve skin aging and restore skin elasticity, and has the effects of whitening skin and whitening skin. The skin moisture content is increased, the skin moisture evaporation amount is reduced, the pre-collagen secretion amount is increased, the collagen biosynthesis is promoted, and the skin redness and sensitive symptoms are relieved.