Application of lysophosphatidic acid

A technology of lysophosphatidic acid and inflammatory reaction, applied in the fields of medicine and biology, can solve the problems of few clinical applications and inability to reach the wound surface, and achieve the effect of promoting skin wound repair and good application prospects.

Inactive Publication Date: 2019-04-19
WEIFANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wound healing is a complex and dynamic process. Many growth factors regulate the wound healing process, but because it is easily degraded by protease or cannot reach the wound surface, it is rarely used clinically.

Method used

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  • Application of lysophosphatidic acid
  • Application of lysophosphatidic acid
  • Application of lysophosphatidic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The skin injury animal model was used to observe the effect of LPA on mouse skin cut wounds. Two cut wounds on the back of the same mouse were treated with physiological concentration (20 μM) of LPA (medication group) and normal saline (control group, Untreated group) respectively. ) treatment, applied to the wound surface, and changed the dressing every day. The results on days 0, 3, 6, 9 and 12 are as follows figure 1 As shown, the upper part is the LPA treatment group, and the lower part is the normal saline control group. On the 6th, 9th, and 12th days, it can be clearly observed that the skin wounds on the back of the mice become smaller and the healing time is shortened. Therefore, LPA can promote the healing of mouse skin cut wounds, shorten the wound healing time, and reduce the inflammatory response.

[0028] Nude mice were used as experimental animals, and the wound skins of nude mice in the treatment group and control group were taken for 12 days, and treat...

Embodiment 2

[0034] LPA can interfere with the changes of inflammatory factors in the trauma model. Hacat epidermal cells were used as a model to detect the expression levels of IL-1 and IL-6 after treatment with different concentrations of LPA for 6, 12, and 24 hours. The experimental method is as follows:

[0035] 1. Hacat (human immortalized epidermis) cell culture

[0036] (1) Wash cells with PBS, centrifuge after trypsinization; resuspend cells with complete medium;

[0037] The complete medium is: MEM medium + 10% fetal bovine serum + 1% double antibody + 1mM sodium pyruvate + 0.1mM non-essential amino acids;

[0038] (2) Plant the culture plate evenly after blowing and mixing, and place at 37°C, 5% CO 2 Cultured in an incubator.

[0039] Second, dosing

[0040] (1) When the cells in each well of the observed culture plate (six-hole plate) grow to 70%-80%, the culture medium is sucked out, and the PBS is sucked out after washing with PBS;

[0041](2) Add 1 mL of serum-free MEM m...

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Abstract

The invention belongs to the technical field of medicine and biology, and discloses application of lysophosphatidic acid. Lysophosphatidic acid can be used for regulating expression of inflammatory factors such as IL-1 and/or IL-6, and inflammatory responses are alleviated; meanwhile, collagen secretion by fibroblasts and epidermis proliferation and regeneration can be promoted. Therefore, the lysophosphatidic acid can repair wounds and promote wound healing and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of medicine and biotechnology, and relates to the application of lysophosphatidic acid in regulating the expression of inflammatory factors. Background technique [0002] Wound healing after human skin trauma is a major problem in the field of wound healing, and there is a lack of effective treatment options. Clinical data show that wounds caused by certain factors do not heal for a long time, which is a difficult problem in clinical treatment. The mechanism of wound healing is an extremely complex process, which is affected by multiple factors such as inflammatory response, vascular permeability, and various cytokines. Wound healing is the physiological response of body tissues to injury stimuli, including four stages: hemostasis, inflammation, proliferation and remodeling. This process requires comprehensive regulation of various growth factors and cytokines, so that the wound can heal within a certain period of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/661A61P29/00A61P17/00A61P17/02
CPCA61K9/0014A61K31/661A61P17/00A61P17/02A61P29/00
Inventor 杨晓云
Owner WEIFANG MEDICAL UNIV
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