The invention relates to an
ion pair antiphase
chromatography and
mass spectrometry combined detection method for low-molecule
heparin part degradation products. All components of the low-molecule
heparin part degradation products are detected in a manner of combining
ion pair antiphase
chromatography and high-resolution
mass spectrometer; all the components in a sample are separated through the
ion pair antiphase
chromatography, and a precise molecule weight is obtained through the high-resolution
mass spectrometer, so that
sugar chain sequence information, including structures of two ends, lengths of
sugar chains and substitution numbers of acetyl and
sulfuric acid, of all the components is calculated, so that the structures of the low-molecule
heparin part degradation products can be finely represented. The ion pair antiphase chromatography and
mass spectrometry combined detection method can detect the structures of the two ends, the lengths of the
sugar chains and the substitution numbers of the acetyl and the
sulfuric acid of the low-molecule heparin part degradation products; the problem in the prior art that only an
ultraviolet absorption atlas for degradation of low-molecule heparin parts can be obtained is solved; the ion pair antiphase chromatography and
mass spectrometry combined detection method has a high practicability value in improvement of the detection level of heparin and guarantee of
drug safety.