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Compound having efficacy of promoting collagen secretion and application thereof

A technology of compounds and active ingredients, applied to a compound with the effect of promoting collagen secretion and its application field

Active Publication Date: 2018-09-04
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pharmacological effects of its volatile oil focus on antibacterial, antifungal, anti-inflammatory, antioxidative and insecticidal effects, and there are no reports on the study of its active ingredients

Method used

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  • Compound having efficacy of promoting collagen secretion and application thereof
  • Compound having efficacy of promoting collagen secretion and application thereof
  • Compound having efficacy of promoting collagen secretion and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Preparation of Compound 1:

[0019] Shade-dried purple ginger tuber 4kg was crushed, extracted 3 times by 95% ethanol-water cold immersion, each time for 24 hours, combined the extracts, extracted with petroleum ether when the extract was concentrated until only water remained, and extracted 3 times to obtain petroleum ether extract 55g. Petroleum ether extract (50g) was subjected to normal-phase silica gel column chromatography, and the petroleum ether / ethyl acetate system was eluted according to the following gradient: petroleum ether elution 3L, petroleum ether / ethyl acetate 20:1 elution 9L, petroleum ether / ethyl acetate 15:1 eluted 3L, petroleum ether / ethyl acetate 9:1 eluted 7L, petroleum ether / ethyl acetate 4:1 eluted 3L, petroleum ether / ethyl acetate 3:1 eluted 3L, Petroleum ether / ethyl acetate 2:1 eluted 6L, and after analysis by thin layer chromatography (TLC), they were combined into 20 segments, denoted as P1-P20; among them, P18 (1.619g) was passed thro...

Embodiment 2

[0024] 1. Activity evaluation and cytotoxicity evaluation of compound 1 in promoting HDFa cells to secrete collagen

[0025] Human dermal fibroblasts-adult (HDFa) were purchased from Cascade Biologics.

[0026] HDFa cytotoxicity test: on a 96-well cell culture plate, mix HDFa cells with the drug solution to be tested, set up 3 replicate wells, and set a blank control without drug at the same time, 37 ° C, 5% CO 2 After culturing for 24 hours, the cytotoxicity was detected by the MTS colorimetric method, and the OD value was measured by a microplate reader at a wavelength of 490 nm. Cell viability was calculated.

[0027] Cell survival rate (%) = experimental well OD 490nm / blank well OD 490nm ×100%

[0028] HDFa collagen secretion promotion experiment: HDFa cells were mixed with 10 g / mL drug solution to be tested on a 12-well cell culture plate, and a blank control without drug and a TGF-β positive control were set. 37°C, 5% CO 2 After culturing for 3 days, collect the c...

Embodiment 3

[0037] Whitening cream formula (wt%) containing compound 1:

[0038]

[0039] The cosmetics of the above-mentioned formula of the present invention are prepared according to the conventional method for making cosmetics.

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Abstract

The invention provides a natural product compound 1 cis-3-(3,4-Dimethoxyphenyl)-4-[(E)-2,4,5-trimehoxystyryl]cyclohex-1-ene derived from Zingiber purpureum Rosc., application thereof in anti-aging skin care products and a preparation method thereof. The chemical constituents of an ethanol extract of Zingiber purpureum Rosc. are chemically separated to obtain the monomeric compound, and the activity of the monomeric compound for promoting collagen secretion of human dermal fibroblasts-adult (HDFa) is evaluated and analyzed. The compound 1 has the half effective concentration EC50 (mug / mL) of 13.906 plus or minus 1.562, shows no cytotoxicity to normal HDFa cells, and thus has application value in skin care products.

Description

Technical field: [0001] The invention belongs to the technical field of cosmetics and medicine, and in particular relates to a natural product compound 1 isolated from traditional Chinese medicinal plants: cis-3-(3,4-Dimethoxyphenyl)-4-[(E)-2, 4,5-trimehoxystyryl]cyclohex-1-ene, which has the activity of promoting HDFa cells to secrete collagen, and the preparation method and application of the compound. Background technique: [0002] Anti-aging has been what people have been pursuing since ancient times, and with the improvement of people's living standards, how to resist the aging of the appearance has attracted more and more attention. Therefore, the development of healthy and safe anti-aging skin care products has attracted more and more attention. According to the global consumption situation, anti-aging skin care products occupy a relatively high proportion in the consumption of skin care products. [0003] The skin is composed of the epidermis, dermis and subcutaneou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C43/215C07C41/38C07C41/36A61K8/33A61Q19/08
CPCA61K8/33A61Q19/08C07B2200/09C07C41/36C07C41/38C07C43/215C07C2601/16
Inventor 杜芝芝李明祥白雪张红霞马永鹏李志坚王雨华解勇王雅琴许艳勇李一泽
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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