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Novel medicine composition capable of stimulating collagen secretion and uses thereof

A composition and drug technology, applied in the field of biomedicine, to achieve the effects of drug safety, promotion of vascularization, and a wide range of sources

Inactive Publication Date: 2017-03-15
SOUTH CHINA INSTITUDE OF BIOMEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Similarly, facial depressed scars caused by factors such as acne and trauma have also brought infinite troubles and pains to people.

Method used

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  • Novel medicine composition capable of stimulating collagen secretion and uses thereof
  • Novel medicine composition capable of stimulating collagen secretion and uses thereof
  • Novel medicine composition capable of stimulating collagen secretion and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] In this example, the inventors screened mesenchymal stem cells from different sources with collagen or hyaluronic acid or calcium hydroxyapatite (microcrystalline porcelain) or polylactic acid or adipose or platelet-rich plasma. The effect of improving cell activity. In this example, the cells to be increased are fibroblasts. Mesenchymal stem cells from different sources include: cord blood, peripheral blood, umbilical cord, placenta, Derived from amniotic membrane, derived from bone marrow, derived from periosteum, derived from fat, derived from teeth, etc. The inventor found that compared with mesenchymal stem cells from other sources, mesenchymal stem cells derived from umbilical cords are medical wastes, with lower cellular immunogenicity and certain versatility. The obtained cells are of high quality and large quantity. , The purity is also higher. Furthermore, in subsequent experiments, the inventors selected a pharmaceutical composition comprising umbilical cord-...

Embodiment 2

[0062] In this example, the inventor observed and identified the morphology and phenotype of the selected umbilical cord mesenchymal stem cells. The results are as follows:

[0063] The morphology of umbilical cord mesenchymal stem cells is that the cells adhere to the wall and grow in the shape of a spindle and sheet. Specific form such as figure 1 Shown. Umbilical cord mesenchymal stem cells are in such figure 1 In the growing state, the umbilical cord mesenchymal stem cells have strong proliferation ability, secrete cytokines including bFGF, VEGF, IGF, etc., and have strong proliferation ability of collagen, and are suitable for selection as the umbilical cord mesenchyme of the pharmaceutical composition of the present invention stem cell.

[0064] Flow cytometric identification of the obtained umbilical cord mesenchymal stem cells, the results figure 2 Shown from figure 2 It can be seen from the results that umbilical cord mesenchymal stem cells express CD90 (positive cell ...

Embodiment 3

[0066] In this embodiment, the inventor further pre-stimulated the obtained umbilical cord mesenchymal stem cells with TGF-β1, bFGF or a combination of the two. The pre-stimulated mesenchymal stem cells were injected into the mouse dermis and subcutaneous fat layer. The inventor found that this is more conducive to the secretion of collagen, more effectively activates autologous cells and stimulates the formation of blood vessels, so that more nutrients are transported to the skin of wrinkles. In this embodiment, the specific dosage of 2-20ng / ml TGF-β1, 5-30ng / ml bFGF, or both are added to pretreat umbilical cord mesenchymal stem cells. The pretreatment time is 1-5 days.

[0067] The inventors found that the umbilical cord mesenchymal stem cells induced to differentiate 3D in the medium supplemented with TGF-β1 and bFGF are as follows image 3 As shown, it can be seen from the results that compared with the normal morphology of mesenchymal stem cells, the induced differentiation...

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Abstract

The invention provides a medicine composition and uses thereof. The medicine composition comprises: mesenchymal stem cells and soft tissue filler, wherein the soft tissue filler comprises at least one of collagen, hyaluronic acid, calcium hydroxyapatite, polylactic acid, fat and platelet rich plasma, and the mesenchymal stem cells are in contact with collagen stimulating factors in advance. The medicine composition is used for improving cell activity such as fibroblast activity. Compared with the prior art, the medicine composition has the advantages of low immunogenicity, quick effectiveness, good naturalness, good treatment effect, more permanent effect and low risk. The medicine composition can be effectively used for removing wrinkles, resisting aging and scars, and repairing wounds.

Description

Technical field [0001] The present invention relates to the field of biomedicine. Specifically, the present invention relates to pharmaceutical compositions and uses thereof. Background technique [0002] Since ancient times, beauty has been an eternal topic. However, as the years go by, no matter how you maintain and care, it cannot prevent the natural sagging and wrinkles of the skin. This makes people, especially many beauty-conscious ladies, show aging, weakened self-confidence and personal charm, and produce negative emotions, which affects the quality of work and life. Similarly, facial depression scars caused by factors such as acne, trauma, etc. have also brought people unlimited trouble and pain. [0003] From the perspective of skin structure, the thickness of dermal tissue is closely related to the amount of fibrous tissue and matrix, and is closely related to the compactness, fullness, sagging and wrinkling of the skin. In recent years, more and more cosmetic skin sci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K47/36A61K47/46A61K47/34A61K47/42A61K47/02A61P17/00A61L27/38A61L27/50A61L27/54
CPCA61K35/28A61K47/02A61K47/34A61K47/36A61K47/42A61K47/46A61L27/3834A61L27/3895A61L27/50A61L27/54A61L2300/412A61L2400/06
Inventor 裴雪涛何丽娟房芳姚海雷南雪岳文张博文
Owner SOUTH CHINA INSTITUDE OF BIOMEDICINE
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