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Plukenetia volubilis L. stem leaf extract capable of delaying senescence as well as preparation method and application of extract

A technology for delaying aging and star oil vine, which is applied in the fields of health, anti-aging beauty cosmetic compositions, health food or skin care, and can solve problems such as unreported anti-aging effects.

Active Publication Date: 2015-03-04
XISHUANGBANNA TROPICAL BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there have been reports on the antioxidant effect of star oil vine leaves, but there is no report on its anti-aging effect

Method used

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  • Plukenetia volubilis L. stem leaf extract capable of delaying senescence as well as preparation method and application of extract
  • Plukenetia volubilis L. stem leaf extract capable of delaying senescence as well as preparation method and application of extract
  • Plukenetia volubilis L. stem leaf extract capable of delaying senescence as well as preparation method and application of extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A star oil vine leaf extract with anti-aging effect, which is prepared by the following method.

[0021] (1) Take the stems and leaves of star oil rattan, clean them, remove impurities, dry them in the shade, put them in an extraction container, add 10 times the amount of methanol solvent with a concentration of 50%, extract 10 times, extract 1 day each time, combine the extracts, and filter , the filtrate was obtained, concentrated under reduced pressure, and evaporated to dryness to obtain the crude extract of the stem and leaf of the star oil rattan, which was set aside.

[0022] (2) Take the crude extract of the stem and leaf of the star oil rattan prepared in the above step (1), add appropriate amount of water to suspend it, and extract it with petroleum ether several times to obtain the extract, concentrate it, and obtain the extract of the stem and leaf of the star oil rattan ,spare.

[0023] (3) The extract prepared in step (2) is subjected to chromatography, a...

Embodiment 2

[0025] A star oil vine leaf extract with anti-aging effect, which is prepared by the following method.

[0026] (1) Take the stems and leaves of star oil rattan, clean them, remove impurities, dry them in the shade, put them in an extraction container, add 3 times the amount of ethanol solvent with a concentration of 70%, extract 4 times, and extract 2 days each time, combine the extracts, and filter , the filtrate was obtained, concentrated under reduced pressure, and evaporated to dryness to obtain the crude extract of the stem and leaf of the star oil rattan, which was set aside.

[0027] (2) Take the crude extract of the stem and leaf of the star oil rattan prepared in the above step (1), add appropriate amount of water to suspend it, and extract it with petroleum ether solvent several times to obtain the extract, concentrate it, and obtain the extraction of the stem and leaf of the star oil rattan things, spare.

[0028] (3) The extract prepared in step (2) is subjected ...

Embodiment 3

[0030] Human leukocyte elastase inhibition assay.

[0031] The extract of the stem and leaf of the star oil vine of Example 2 was tested in the human leukocyte elastase inhibition experiment. The positive activity in the test confirms the anti-aging activity of the extract.

[0032] Experimental method: In a 96-well plate, 25 μl of 0.1 M HEPES buffer, sample (2 mg / ml) and elastase (1un / ml) were added to the experimental group; 75 μl of HEPES buffer was added to the blank group, and 75 μl of HEPES buffer was added to the negative control group 25 μl elastase and 50 μl HEPES buffer; positive control group was added 25 μl elastase, HEPES buffer and elastin (10 μg / ml); solvent control group was added 25 μl elastase, HEPES buffer and Solvent to dissolve samples; Sample Color Control contains 150 μl HEPES buffer and 25 μl sample. After the 96-well plate was incubated at room temperature for 20 minutes, 100 μl of substrate was added respectively, and then incubated at 25° for 40 mi...

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Abstract

The invention provides a plukenetia volubilis L. stem leaf extract capable of delaying senescence as well as a preparation method and an application of the extract in the aspects of cosmetics, health food and the like. The invention also relates to a cosmetic composition which is used for resisting oxidation, preventing skin aging and removing wrinkles and contains the plukenetia volubilis L. stem leaf extract as an active ingredient. The plukenetia volubilis L. stem leaf extract has inhibition activity on elastinase, collagenase and hyaluronidase and has significant effects in collagen secretion promoting experiments. The usage amount of the extract in health food or anti-aging cosmetics can be 0.01wt%-100wt%.

Description

technical field [0001] The present invention relates to an anti-aging plant extract (crude extract, extract, refined extract), its preparation method and application. In particular, it relates to star oil vine leaf extract with anti-aging activity, and relates to a preparation method containing the extract as an active ingredient and health, health food or skin care, anti-aging cosmetic cosmetic composition, and the composition can be used for anti-aging and improve skin wrinkles. Background technique [0002] my country is one of the countries with the fastest aging rate in the world and one of the countries with the largest aging population. Aging is a complex phenomenon of life, which refers to the gradual process of functional and organic decline that occurs in all individuals over time. As the largest organ of the human body, the skin covers the whole body of the human body. While playing a protective role, it is also the most vulnerable to external damage. Its aging...

Claims

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Application Information

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IPC IPC(8): A61K36/47A61K8/97A61Q19/08A61P39/06A23L1/30A23L33/105
CPCA23V2002/00A61K8/97A61K2800/782A61Q19/08A23V2200/302A23V2250/21
Inventor 郑小玲许又凯
Owner XISHUANGBANNA TROPICAL BOTANICAL GARDEN CHINESE ACAD OF SCI
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