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Application of active peptide and mesenchymal stem cell exosome for improving skin physiological characteristics in drugs or cosmetics

A technology of quality stem cells and physiological characteristics, applied in the direction of cosmetics, cosmetic preparations, skin care preparations, etc., can solve the problems of poor stability of skin care effects, complex and limited components of stem cell products, etc., to improve antioxidant capacity and promote Rapid recovery of normal physiological functions and promotion of skin tissue repair

Active Publication Date: 2021-08-27
珠海医美企业管理有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the composition of stem cell products is complex, and the preparation process needs to be strictly controlled. The skin care effect of using stem cells or their derivatives alone is not stable, which limits their application in skin care cosmetics.

Method used

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  • Application of active peptide and mesenchymal stem cell exosome for improving skin physiological characteristics in drugs or cosmetics
  • Application of active peptide and mesenchymal stem cell exosome for improving skin physiological characteristics in drugs or cosmetics
  • Application of active peptide and mesenchymal stem cell exosome for improving skin physiological characteristics in drugs or cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Design and Preparation of Active Peptides for Improving Skin Physiological Properties

[0034] 1.1 Design of improved active peptides

[0035] In the previous work, the inventor obtained an active peptide that can improve skin physiological activity and resist ultraviolet damage. Its amino acid sequence is shown in SEQ ID NO.1, and it was named SDH peptide.

[0036] SEQ ID NO: 1: EVKLGNKLSPALGEPLCTSHK

[0037]In order to further improve its antioxidant and value-promoting activities, the inventors introduced site-directed mutations into the relevant active sites based on the amino acid structure and mechanism of action of the peptide, using computer simulation and bioinformatics methods. Substitution of existing amino acids, such uncommon amino acids include but are not limited to β-alanine (β-Ala) and other omega-amino acids such as 3-aminopropionic acid, 2,3-diaminopropionic acid (Dpr), 4-aminobutyric acid, etc.; α-aminoisobutyric acid (Aib), ε-aminocaproi...

Embodiment 3

[0054] Example 3 LDH peptide and mesenchymal stem cell exosomes enhance the ability of epithelial cells to resist oxidative stress

[0055] 3.1 Preparation of UV-damaged HaCaT cell model

[0056] DMEM medium containing 10% FBS, 37°C, 5% CO 2 HaCaT cells were cultured in an incubator. Take the HaCaT cells in good condition and in the exponential growth phase, digest, count, and inoculate the cells in a 96-well plate at a seeding concentration of 1×10 5 per well, placed at 37°C, 5% CO 2 Continue culturing in the incubator for 12 hours. Irradiate HaCaT cells with UVB (311nm) at an intensity of 50mJ / cm 2 , to establish a UV damage model.

[0057] 3.2 Cell survival test

[0058] After the ultraviolet damage model was prepared, the above cells were divided into 6 groups, namely: blank control group, HaCaT cells without UVB irradiation; the remaining 5 groups were irradiated cells, namely: PBS group, adding 100 μL PBS; SDH group , add 100 μL 10mg / mL SDH polypeptide to each wel...

Embodiment 4

[0066] Example 4 LDH peptide and mesenchymal stem cell exosomes protect skin damage in rat models

[0067] 4.1 Preparation and treatment of ultraviolet damage model in rats

[0068] Select healthy SD rats, SPF grade, half male and half female, body weight 180±20g, randomly divided into 6 groups, 10 rats in each group, including blank control group, normal saline group, SDH group, LDH group, combined group 1 and combined Group 2. The hair on the back of rats in each group was shaved, and the area was about 5cm×5cm. The rats were placed on a fixed platform and irradiated under an ultraviolet lamp (wavelength: 311nm) at a distance of about 10cm. 12 weeks, in which the blank control group did not receive ultraviolet radiation.

[0069] Before each ultraviolet irradiation, apply therapeutic agents to the depilated parts, specifically: the blank control group, no ultraviolet irradiation, and no treatment; the normal saline group, apply 500 μL of normal saline; the SDH group, apply...

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Abstract

The invention belongs to the technical field of biological pharmacy, and particularly relates to application of active peptide and a mesenchymal stem cell exosome for improving skin physiological characteristics in drugs or cosmetics. Amino acid replacement is performed on the basis of original active peptide, and other conventional amino acids and unusual amino acids are introduced, so that the antioxidant capacity, collagen secretion promoting capacity and cell proliferation promoting capacity are improved. The effect of combined use between the amino acids and the human umbilical cord mesenchymal stem cell exosome is more obvious, skin injuries caused by ultraviolet rays can be effectively prevented and treated, and the repair process after the injuries is promoted.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of an active peptide for improving skin physiological characteristics and mesenchymal stem cell exosomes in medicine or cosmetics. Background technique [0002] Skin aging is an important topic in the research of skin care and skin care cosmetics. Skin aging will not only seriously affect the appearance, but also may cause specific symptoms such as dryness, roughness, telangiectasia, decreased elasticity, irregular pigmentation, and seborrheic keratosis. . According to the mechanism of action, skin aging is mainly divided into two categories: endogenous aging and exogenous aging. Endogenous aging is a physiological process affected by genes, hormones, etc. Aging caused by stimulation, ultraviolet rays are an important factor leading to skin aging. [0003] Ultraviolet rays can be divided into three bands according to the wavelength, namely, long-wave ult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/00A61K8/64A61K8/99A61Q19/00A61Q19/08
CPCC07K14/001A61K8/64A61K8/99A61Q19/08A61Q19/00A61K2800/592
Inventor 王京张然
Owner 珠海医美企业管理有限公司
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