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Reversible collagen stimulating filling agent and preparation method thereof

A filler and collagen technology, applied in the fields of soft tissue filling and medical beauty filling, to achieve good application prospects, environmental protection of technical routes, and the effect of stimulating collagen secretion

Active Publication Date: 2018-10-16
SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a reversible injectable collagen stimulating filler and its preparation method to solve the deficiencies of existing related products and technologies

Method used

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  • Reversible collagen stimulating filling agent and preparation method thereof
  • Reversible collagen stimulating filling agent and preparation method thereof
  • Reversible collagen stimulating filling agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Add hydroxybutyl chitosan with a concentration of 8.0 mg / mL and a hydroxybutyl substitution degree of 2.0 to the oil phase, the ratio of which to the oil phase is 7 wt%. Then 0.2wt% surfactant Tween 80 was added dropwise. Stir first at 5°C for 1 hour and then at 80°C for 1 hour. The oil phase was removed by suction filtration, and the collected microspheres were soaked in physiological saline at 80° C. for 24 hours. Screen particle size. Observe the shape of the pellets under a microscope.

[0027] Add the obtained hydroxybutyl chitosan microspheres into the hyaluronic acid gel at a ratio of 20%. Hyaluronic acid has a molecular weight of 10 6 Da, the concentration is 1.6wt%, the dynamic viscosity is 1.6*10 6 mPa s. It was then stirred at 500 rpm for 1 hour. Autoclave for 15 minutes and fill. The dynamic viscosity of the hybrid gel was tested by a rheometer.

[0028] like figure 1 As shown, the prepared microspheres have a good spherical shape and a smooth surf...

Embodiment 2

[0030]Take hydroxybutyl chitosan microspheres with a particle size of 20-30 μm, accurately weigh a certain mass and put it into a test tube. Prepare 2mg / mL lysozyme (20000U / mg) degradation solution. Add the degradation solution to the microspheres at a ratio of 10mg / g, replace it twice a day, take samples every day for 5 days, and weigh the mass after drying. The degradation rate was calculated according to the following formula:

[0031] Degradation rate (%) = (W 0 -W t ) / W 0 *100%

[0032] Among them, W 0 is the mass of microspheres before degradation, W t is the mass of remaining microspheres after degradation.

[0033] like image 3 As shown, under the conditions of accelerated degradation in vitro, the hydroxybutyl chitosan microspheres were degraded by 36% and 54% on the 1st and 2nd day, respectively, and about 90% by the 5th day. The above results show that hydroxybutyl chitosan microspheres can be rapidly degraded under the action of high concentration of lyso...

Embodiment 3

[0035] Add hydroxybutyl chitosan with a concentration of 10.0 mg / mL and a hydroxybutyl substitution degree of 1.7 to the oil phase, the ratio of which to the oil phase is 10 wt%. Then 0.2wt% surfactant Tween 80 was added dropwise. Stir first at 5°C for 1 hour and then at 80°C for 1 hour. The oil phase was removed by suction filtration, and the collected microspheres were soaked in physiological saline at 80° C. for 24 hours. Screen particle size. Observe the shape of the pellets under a microscope.

[0036] Add the obtained hydroxybutyl chitosan microspheres into the hyaluronic acid gel at a ratio of 10%. The molecular weight of hyaluronic acid is 1.3*10 5 Da, the concentration is 1.4wt%, the dynamic viscosity is 1.7*10 6 mPa s. Then it was stirred at 500 rpm for 1.5 hours. Autoclave for 15 minutes and fill.

[0037] The particle size of hydroxybutyl chitosan microspheres obtained in this example is 25-40 μm, and the degree of substitution of hydroxybutyl group decreas...

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Abstract

The invention discloses a reversible collagen stimulating filling agent and a preparation method thereof. By utilizing temperature-sensitive and ion response characteristics of hydroxybutyl chitosan,formation, separation and curing of microspheres are promoted, and the degradation time is prolonged; by utilizing positive and negative charge attraction between hyaluronic acid carrier gel and hydroxybutyl chitosan microspheres, uniform and stable dispersion of the microspheres can be promoted, and agglomeration of the microspheres can be effectively prevented. The preparation process is simplein steps, environmentally friendly in technical route, low in cost and favorable for industrialization. The prepared reversible collagen stimulating filling agent has long degradation period under normal physiological conditions, can effectively stimulate collagen secretion for a long time, particularly has an effective emergency degradation mechanism, and can be rapidly degraded by taking measures during overcorrection or misoperation, and risk of product injection is reduced. The reversible collagen stimulating filling agent provided by the invention has effectiveness and safety, and has excellent application prospects in the soft tissue filling field, particularly medical cosmetology filling.

Description

technical field [0001] The invention relates to the field of tissue filling and medical biomaterials, in particular to a reversible injectable collagen stimulating filler and a preparation method thereof, which are mainly used in soft tissue filling, especially medical cosmetic filling. Background technique [0002] In recent years, injectable collagen-stimulating fillers have gradually received widespread attention in the field of medical aesthetics. This type of filler often contains a polymer hydrogel carrier and a certain proportion of particle microspheres with a long degradation period. Studies have shown that after injecting this type of filler into the human body, it will first cause a mild initial inflammatory response, and the microspheres will be gradually wrapped by fibroblasts, and then fibrous proliferation will form a stable collagen without scar tissue (mainly type I). ). Moreover, the encapsulation will continue until the microspheres are completely degrad...

Claims

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Application Information

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IPC IPC(8): A61L27/50A61L27/26A61L27/24
CPCA61L27/24A61L27/26A61L27/50A61L2400/06A61L2430/34C08L5/08
Inventor 魏长征王晓彤蒋丽霞
Owner SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD
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