116 results about "High serum" patented technology

The invention discloses a complete medium with low serum concentration for cultivating mesenchymal stem cells and a method for cultivating the mesenchymal stem cells using same. The complete medium comprises a cell basic medium, fetal calf serum with final concentration of 1-100 mul / ml, an epidermal growth factor with final concentration of 1-100 ng / ml, and a basic fibroblast growth factor with final concentration of 1-100 ng / ml. The complete medium with low serum concentration successfully reaches equal or even better function of promoting cell proliferation than a culture reagent with high serum concentration. The cultured cells have the typical biological characteristics of mesenchymal stem cells, and can also express an omnipotent mark of the embryonic stem cell and high express the idiosyncratic mark of the neuron under the condition of in vitro inducement. And the difference between the cell batches is little, the cost is low and the security is good. Compared with the prior cultivating method, the method has advantages of simple operation, low probability of pollution and high success ratio of cultivating cells.

Methods of treating high serum levels of total cholesterol, low density lipoprotein and triglycerides in a mammal by administering therapeutically effective doses of N-(4-{[4-(3,4-dichlorophenyl)-1,3-thiazol-2-yl]amino}phenyl)acetamide or a salt thereof such as a hydrochloride or hydrobromide salt.

Statin therapy has revolutionized the treatment of cardiovascular disease, but not all patients can take the appropriate level of statins because of their side effects. The present invention provides compositions that provide potent cholesterol lowering while minimizing the damaging side effects to liver, muscles, and neurons, and has the added benefit of reducing chronic systemic inflammation, which is an independent determinant of cardiovascular disease and all-cause mortality. The current invention presents pharmaceutical compositions for reducing cholesterol and chronic systemic inflammation comprising therapeutically effective amounts of: at least one lipid-lowering agent chosen from HMG-CoA reductase inhibitors, high-dose controlled-release niacin, red yeast rice, or policosanol; and at least one antiinflammatory natural product chosen from alpha-lipoic acid and corosolic acid. To those in need of such treatment, the current invention also provides safe methods for reducing high serum cholesterol or chronic inflammation, or for simultaneously reducing both cholesterol and chronic inflammation via treatment with therapeutically effective daily doses of pharmaceutical compositions as described herein. The present invention provides mammals with compositions and methods for concurrently reducing cholesterol and inflammation as a prevention or treatment for many age-related diseases and disorders.

The invention provides a PED (Porcine Epedemic Diarrhea) inactivated vaccine and a preparation method thereof and relates to the field of biopharmacy. The PED inactivated vaccine comprises inactivated PEDV (Porcine Epedemic Diarrhea Virus), and is characterized in that the PED inactivated vaccine comprises 0.05-10 mg / mL Beta-glucosylceramide, 0.1-21 mg / mL monophosphoryl lipid A, 1.5-125 mg / mL muramyl dipeptide and 0.7-4.5 mg / mL Beta-glucan. According to the ingredients of the PED inactivated vaccine, Beta-glucosylceramide, monophosphoryl phosphoryl lipid A, muramyl dipeptide and Beta-glucan have the synergistic effect, the immune response of animals to antigens in the vaccine is significantly improved, the immune window phase is shortened, the antibody production duration of the animal body is obviously prolonged, the serum antibody level is improved, and the level of total intestinal mucosa secretory antibodies (the total SIgA) is improved.

The invention provides a coffee mate product prepared from pine nuts, belongs to the field of a beverage processing biotechnology, and relates to a coffee mate product adopting pine nut oil microcapsules to be matched with coffee drinks. The coffee mate product is prepared from 60%-80% by weight of pine nut oil microcapsule powder and 20%-40% by weight of sucrose. The pine nut oil microcapsule powder of the coffee mate contains rich unsaturated fatty acid and especially has the highest linoleic acid content; the unsaturated fatty acid can promote cholesterol metabolism, reduce blood lipid and increase the content of high-density lipoprotein in serum, prevent lipid deposition on a liver and an arterial wall, reduce platelet cohesion and reduce thrombosis, and the coffee mate product prepared from the pine nuts is ideal food for protection of blood vessels of the crowd with hyperlipidemia.

Methods and composition for generation of arginase variants with high serum persistence are provided. For example, in certain aspects methods for purifying pegylated arginase are described. Furthermore, the invention provides stabilized arginase multimers or pharmaceutical composition thereof.

The invention belongs to the field of Chinese medicine pharmacy, and relates to a traditional Chinese medicine composition for treating menopausal syndrome and a preparation method of the traditional Chinese medicine composition. The traditional Chinese medicine composition comprises traditional Chinese medicine radix rehmanniae, radix paeoniae alba, rhizoma anemarrhenae, golden cypress, morinda officinalis, herba epimedii, raw oyster shell, crude dragon bone, radix bupleuri and tuber fleeceflower stem as main medicines, and is blended with auxiliary materials to be prepared into the preparation for treating the symptoms related to menopause, i.e. the heating, the sweating, the agitated emotion, the insomnia and the like. The experiment proves that the traditional Chinese medicine composition provided by the invention has the definite functions for improving the blood serum E2 and reducing the blood serum FSH (follicle-stimulating hormone), the hypothalamus estrogen receptors Er alpha, Er beta gene expression and protein expression can be continuously adjusted, and the situation of hypothalamus aging can be improved, so that the menopausal syndrome can be effectively cured.

The invention relates to the biomedical field, and particularly relates to application of dihydromyricetin in preparation of a liver treatment and regeneration promotion medicine. Dihydromyricetin shows relatively high oxidation resistance and characteristic on promoting cell growth as a flavonoid compound. The test shows that dihydromyricetin can effectively relieve liver tissue necrosis caused by acute hepatic failure and can promote hepatocyte proliferation, so as to obviously raise the survival rate of a model mouse suffering from acute hepatic failure; dihydromyricetin also can obviously improve the activity of superoxide dismutase in tissue and serum, increase the content of serum protein, and lower down the levels of glutamic oxalacetic transaminase, glutamic-pyruvic transaminase and tumor necrosis factor alpha.

An influenza vaccine adjuvanted with a sub-micron oil-in-water emulsion elicits significantly higher immune responses in human pediatric populations. Compared to an existing unadjuvanted pediatric influenza vaccine, the adjuvanted vaccines provided herein can induce in children a longer persistence of high serum antibody titers and also longer seroconversion and seroprotection. The improvement in immune responses is seen for both influenza A virus and influenza B virus strains, but it is particularly marked for influenza B virus. Moreover, while the existing vaccine provides poor immunity in children after a single dose, the adjuvanted vaccine provides high seroprotection rates against the influenza A virus H3N2 subtype even after a single dose. Furthermore, the adjuvanted vaccine offers significantly better seroprotection against mismatched strains of influenza A virus.

The invention discloses a preparation method of an alpha-glucan antigen and a specificity monoclonal antibody. The preparation method of the antigen comprises the following steps: mixing alpha-glucan and protein; dissolving with water; regulating the pH of solution to 5-8; carrying out freeze drying, and grinding into powder; putting the powder in the environment with constant temperature of 50-60DEG C and relative humidity of 60-90%; reacting for at least three days to obtain the alpha-glucan antigen. The preparation method of the antibody comprises the following steps: mixing the prepared antigen with an adjuvant, emulsifying the mixture, and injecting the emulsified mixture into animal bodies; blending the splenocyte and oncocyte of the animal body with the high serum valence to obtain a hybridoma cell capable of secreting the antibody; and producing the antibody by the hybridoma cell or with an ascites method. The antibody prepared by the method has high immunogenicity, and can be used for effectively inducing to generate immune response to obtain the antibody with the excellent property. The antibody obtained by the method provided by the invention can be subjected to specificity reaction with the alpha-glucan to generate macroscopic precipitates, and the antibody has high valence and can be used for the quantitative analysis on the alpha-glucan.

The invention discloses application of brown cyanidin in preparing a medicament for treating high blood fat diseases, in particular a medicament for treating high blood fat diseases of high triglyceride and high serum low-density protein types. The applicant discovers that the brown cyanidin has remarkable curative effect. Meanwhile, the invention also provides a preparation for treating the diseases.

The invention discloses an application of inonotus obliquus polysaccharide used as a feed additive for litopenaeus vannamei, and the addition amount of the inonotus obliquus polysaccharide in a litopenaeus vannamei basal feed is 500-1500 mg / kg. The fact that the inonotus obliquus polysaccharide is used as the additive for the litopenaeus vannamei feed can substantially raise the weight gain rate and the specific growth rate of the litopenaeus vannamei, substantially reduce the feed coefficient, meanwhile substantially increase lysozyme and alkaline phosphatase in serum of the litopenaeus vannamei and raise total antioxidant capacity of the litopenaeus vannamei, increase nitric oxide synthetase and superoxide dismutase in the serum, and reduce the content of malonaldehyde in the serum. The inonotus obliquus polysaccharide has an equivalent action effect to beta glucan, and is a new-type immunopotentiator, and has a broad application prospect in the aquaculture field of the litopenaeus vannamei.

The invention discloses a polystictus glycopeptide active component as well as a preparation method and application thereof. The polystictus glycopeptide active component comprises the following steps: preparation of coriolus versicolor mycelium; extraction of the polystictus glycopeptide active component from the coriolus versicolor mycelium; separation, purification and enzymatic hydrolysis of the polystictus glycopeptide active component. The active component is found by comparing the therapeutical effect of the polystictus glycopeptide active component on an acute alcoholic liver injury mouse model to better reduce the vitalities of AST and ALT in serum, reduce the contents of Triglycerid, MDA, NEFA, TC and LDL-TC in the serum, improve the content of HDL-TC in the serum and realize thebetter therapeutical effect of resisting alcoholic liver injury. According to the preparation method disclosed by the invention, active functional factors for preventing and treating the alcoholic liver injury are found from natural food resources; the obtained polystictus glycopeptide active component can reduce indicators of the ALT, the AST, cholesterol, free fatty acid and the like in the serum, and has a good application value and a potential market prospect in aspects of preventing and treating alcoholic liver disease, fatty liver or hyperlipidemia.

The invention provides a milk powder product prepared from pine nuts, belongs to the field of a beverage processing biotechnology, and relates to a milk powder product adopting pine nut oil microcapsules. The milk powder product is prepared from 30%-40% by weight of pine nut oil microcapsule powder and 60%-70% by weight of milk powder. The pine nut oil microcapsule powder of the milk powder contains rich unsaturated fatty acid and especially has the highest linoleic acid content; the unsaturated fatty acid can promote cholesterol metabolism, reduce blood lipid and increase the content of high-density lipoprotein in serum, prevent lipid deposition on a liver and an arterial wall, prevent platelet cohesion and reduce thrombosis, and the milk powder product prepared from the pine nuts is ideal food for protection of blood vessels of the crowd with hyperlipidemia.

The present invention belongs to the technical field of health-care products, and particularly relates to an anti-ageing health-care product and a detection method thereof. The health-care product is mainly made from ginseng, caulis spatholobi, milkvetch root, tuber fleeceflower stem, coix seed, fructus corni, Indian bread, cassia twig, glossy privet fruit, dendrobium officinale and liquorice root. The detection method is to use a gas chromatograph to detect a TOSC value of the health-care product, so as to judge the anti-aging ability of the health-care product. The anti-aging health-care product has effects in nourishing liver and kidney and invigorating spleen and replenishing Qi, and enhances human body constitution and body immunity function proceed from the internal cause of aging; at the same time, the health-care product also has strong antioxidant activity, and can effectively remove the free radicals in the body by increasing the activity of SOD in serum and decreasing the activity of MDA in serum, and reduce the damage resulted from the free radicals to the human body to reach beauty-maintaining, countenance-preserving and senescence delaying effects.

The invention discloses a Chinese herbal compound volatile oil part for treating asthma and a preparation method thereof. The Chinese herbal compound volatile oil part is extracted by steam distillation from a crude drug which is made from licorice, Chinese ephedra, bitter apricot kernel, pinellia tuber, gypsum, tea bust and Chinese magnolivine fruit according to certain weight proportion by parts with the yield of 0.17%. The Chinese herbal compound volatile oil part has the advantages of simple preparation process, good reproducibility and low cost. Pharmacological tests prove that the Chinese herbal compound volatile oil part of the invention can obviously prolong the incubation period of asthma in animals, reduce the responsiveness of asthma airway, improve the ethological sign of asthma, obviously decrease EOS in BALF, inhibit the aggregation of main inflammatory cells EOS in lung tissues, obviously inhibit the level of inflammatory factors IL-4 and IL-5 in serum, improve the level of IFN-Gamma in serum, and regulate the balance relationship of Th1 / Th2, therefore, the Chinese herbal compound volatile oil part of the invention is ideal for preparing the medicine for treating asthma.

The invention discloses a special clinical nutrition formula for lymphoma and a preparation method thereof. The special clinical nutrition formula is prepared from the following components in parts byweight: 10-20 parts of fat, 35-60 parts of proteins, 10-25 parts of carbohydrates, 3-10 parts of dietary fibers, 0.11 to 0.3 part of sodium, 0.1 to 0.2 part of potassium, 0.1 to 0.2 part of calcium,0.01 to 0.02 part of iron, 0.01 to 0.02 part of zinc, 0.01 to 0.02 part of magnesium, 0.02 to 0.3 part of composite vitamin, 0.5 to 10 parts of medicinal and edible components, 0.1 to 0.4 part of probiotics and 0.1 to 1 part of new resource food. According to the clinical nutrition formula provided by the invention, by improving the nutritional status of a lymphoma patient, serum albumin is improved, adverse reactions during chemotherapy are relieved, and prognosis of the patient is improved. The later reasonable nutrition support can regulate abnormal metabolism of tumors, inhibit growth of the tumors at the same time, and finally prolong the survival time of patients.

The invention discloses new functions of eleutherine plicata herb and an extract thereof, including reducing blood lipid and preventing hyperlipemia. The function of reducing blood lipid refers to reducing contents of serum cholesterol, triglyceride and low density lipoprotein cholesterin and improving content of high density lipoprotein cholesterin; and the function of preventing hyperlipemia refers to preventing high serum cholesterol, high triglyceride, high content of low density lipoprotein cholesterin, and low content of high density lipoprotein cholesterin.

The invention relates to a cholic acid-XenGLP-1 (xenopus glucagon-like peptide-1) conjugated peptide and an application thereof. Side chains of XenGLP-1 are subjected to structural modification, a cholic acid micromolecular derivative with a high serum protein binding ratio is introduced, and the XenGLP-1 conjugated peptide with higher hypoglycemic activity and longer pharmacological action time is obtained. The XenGLP-1 conjugated peptide has significantly improved bioactivity and high long-term hypoglycemic and weight losing functions.

The invention relates to the technical field of application of neutral polysaccharide from Brassica rapa L., and particularly discloses application of the neutral polysaccharide from Brassica rapa L.in preparation of antioxidant drugs. Indexes in serum and whole brain and liver homogenate of mice in a D-galactose-induced aging mouse model and a control group are determined, respectively. It is found that the neutral polysaccharide from Brassica rapa L. has an antioxidant effect on the D-galactose-induced aging mouse model. The invention finds, for the first time, that the neutral polysaccharide from Brassica rapa L. significantly increases the activity of SOD, T-AOC, CAT, GSH and GSH-Px in serum and whole brain and liver homogenate, and significantly reduces the MDA content, thereby improving antioxidant ability and delaying senescence.

The present invention discloses a functional exercise energy glue and a preparation method thereof, wherein 0.1-5 parts of a phyllanthus emblica linn extract, 0.1-5 parts of a polygonatum odoratum extract, 0.1-5 parts of a tuckahoe extract, 0.1-5 parts of a maca extract, 0.5-5 parts of whey protein, 1-10 parts of wheat oligopeptide, 0.05-0.1 part of taurine, 0.1-0.5 part of glutamic acid, 0.05-3 parts of citric acid, 0.05-3 parts of sodium citrate, 0.15-5 parts of a gelling agent, 30-100 parts of white granulated sugar, 5-35 parts of fructose, 5-35 parts of isomaltooligosaccharide, 0.1-1 part of edible essence, 180-250 parts of purified water are subjected to material preparation, and glue dissolving, sugar dissolving, mixing, acidifying, total mixing, filling, sterilization and other processes are performed to prepare the food functional exercise energy glue. The functional exercise energy glue of the present invention uses the formula of the food having medicine food homology, has the complete nutrition, has effects of rapid and continuous energy supplementing, metabolite accumulation eliminating, serum testosterone increasing, qi yin essence consumption and injury relieving, spleen transport dysfunction relieving and internal dampness-heat generation relieving, has characteristics of convenient carrying and good taste, and is suitable for people doing exercise.

The invention discloses a method for improving the foaming property and foam stability of serum albumin by utilizing chitosan oligosaccharide. The method comprises the following steps: adding chitosanoligosaccharide into serum albumin, performing uniform mixing in a solvent, and regulating the pH of the mixture to be 5-9. The invention further provides a foaming agent. The foaming agent is a serum albumin / chitosan oligosaccharide system, namely a mixed solution with the pH of 5-9 prepared by dissolving serum albumin and chitosan oligosaccharide in the solvent. According to the method for improving the foaming property and foaming stability of serum albumin by utilizing chitosan oligosaccharide, the noncovalent static interaction can be generated from chitosan oligosaccharide and serum albumin by adjusting the pH value, a chitosan oligosaccharide / serum albumin compound is formed, and the foaming property and foam stability of the serum albumin can be remarkably improved with simple combination. The method is simple in process and can reduce time cost.

The invention relates to a liver-protecting tea and a preparation method thereof. Gynostemma pentaphylla, notoginseng flower, scutellaria and momordica are used as raw materials and subjected to fine selection, removal of impurity and rotten and deteriorated raw materials, drying at a temperature of between 50 and 85 DEG C, pulverization and sieving by a vibrating screen respectively; the raw material with 20 to 60 meshes is taken out, mixed according to the proportion, sterilized and packaged to prepare the liver-protecting tea. The liver-protecting tea has the efficacy in clearing heat, resolving toxin, lowering fat, protecting liver, transforming stasis and eliminating dampness and is suitable for fatty liver and mild hyperlipidemia people. An experimental research shows that the liver-protecting tea has the functions of reducing total cholesterol and triglyceride of serum, reduces low-density lipoprotein, increases high-density lipoprotein of serum, improves liver function and liver B ultrasonographic change and has no toxicity and side effect.

The invention discloses recombinant epsilon protein for inhibiting clostridium perfringens infection and a preparation method and application thereof. The recombinant epsilon protein is (a) protein formed by amino acid sequences shown in SEQ ID No.2 or (b) protein formed by amino acid sequences from the 51 site to the 370 site in SEQ ID No.2 or fusion protein obtained by fusing protein tags on carboxyl terminals or / and amino terminals of the (a) protein or the (b) protein. After animals are immune to the recombinant epsilon protein, the animals can generate the high serum antibody level and can resist attack of clostridium perfringens. The recombinant epsilon protein is good in dissolvability and easy to purify and can be taken as a diagnostic antigen or prepared into a monoclonal antibody or used for further studying the protein function and the conformation relationship.

The invention relates to application of lactobacillus plantarum DP189 in preparation of medicine for preventing and / or treating Alzheimer's disease, and belongs to the field of biology, the lactobacillus plantarum DP189 is deposited in China Center for Type Culture Collection on March 25, 2019, and has a deposit number of CCTCC NO: M2019199. The lactobacillus plantarum DP189 is prepared into bacterial suspension with number of viable bacteria greater than or equal to 1 * 10 < 9 > CFU / ml. The lactobacillus plantarum DP189 can improve learning and memory ability, improve cognitive impairment, relieve damage degree of hippocampal neurons, reduce a level of malonaldehyde in serum, improve a level of superoxide dismutase in serum and improve levels of glutathione and glutathione peroxidase in serum; reduces levels of interleukin-1beta and tumor necrosis factor-alpha in serum, increases a level of interleukin-6 in serum, increases a level of dopamine in serum, and increases a level of 5-hydroxytryptamine in serum.

The invention relates to the technical field of food processing, in particular to tablets conducive to improving men's health and relieving fatigue. The tablets comprises raw materials in parts by weight as follows: 50-60 parts of sorbitol, 30-35 parts of oyster peptide, 20-25 parts of fruit powder, 5-10 parts of deer heart blood powder and 2-5 parts of ginseng. According to the tablets conduciveto improving men's health and relieving fatigue, with addition of the oyster peptide and the deer heart blood powder, the anti-fatigue effect of men can be greatly improved; with addition of the fruitpowder, taste can be improved, and the tablets are more easily eaten by men; with addition of sorbitol, storage time of the tablets can be prolonged; and the oyster peptide can enhance the sexual function of the men, effectively increase serum testosterone level and enhance the sexual function of the men, and meanwhile, the oyster peptide has dual functions of adjusting physiological functions ofthe body and improving nutrition of the body and further can protect the liver.

The invention provides novel 18F labeled substituted benzimidazole compounds, which are characterized in that: one end of each compound contains an 18F substituted alkoxy structure, and the other end contains a 6-carboxyl / H benzimidazole structure; a substituent group R1, located on the site 2 of benzimidazole matrix, is hydrogen, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, benzyl, 2-methylthio ethyl and phenyl; a substituent group R2, located on the site 4 of benzimidazole matrix, is hydrogen, methyl and ethyl; and the structure of the compound is shown in formula A, wherein n is between 1 and 5, R2 is H, Me and Et, X is COOH and H. Experiments show that the compounds have high bioactivity such as fast serum removal, high serum stability and relatively low intake in tissues or organs such as liver and relatively high enrichment and slow removal rate in tumor cells, and therefore the compounds have relatively high tumor / background value and are favorable for PET tumor imaging. Meanwhile, the labeled precursor of the compounds is easy to synthesize and the labeling rate is extremely high; and such advantages indicate that the compounds have the tremendous potential to become PET tumor imaging agents.

The present invention relates to the technical field of food and / or pharmaceuticals, and discloses a blood fat lowering composition and a preparation method and application thereof. The blood fat lowering composition comprises Echium vulgare seed oil, camelina seed oil and safflower seed oil. The blood fat lowering composition is a natural composition which comprises specific natural substances the Echium vulgare seed oil, the camelina seed oil and the safflower seed oil as active ingredients, and can significantly reduce total cholesterol and triglyceride and increase serum apolipoprotein A1content. The blood fat lowering composition has an obvious hypolipidemic effect and can be applied to the development and preparation of hypolipidemic drugs and foods, especially health foods.