129 results about "Brassica rapa" patented technology

The invention discloses nitrilase from brassica rapa, encoding genes and an application of the encoding genes to the preparation of pregabalin chiral intermediate (S)-3-cyan-5-methylhexanoic acid through biocatalysis, and an amino acid sequence of the nitrilase is shown as SEQ ID NO:1. The invention provides novel nitrilase for preparing the (S)-3-cyan-5-methylhexanoic acid with high region and high stereoselectivity through hydrolysis, the concentration of hydrolysis substrates of the nitrilase can reach more than 1 M, and an ee (enantiomeric excess) value is kept more than 99 percent. The pregabalin important chiral intermediate (S)-3-cyan-5-methylhexanoic acid can be prepared through the nitrilase; a preparation method of the (S)-3-cyan-5-methylhexanoic acid has the advantages of high atom economy, mild conditions, environmental friendliness and the like and has high industrial application potential.

The invention relates to a usage of Brassica rapa polysaccharide which is extracted from Brassica rapa plants in the preparation of anti-hypoxia drugs, the average molecular weight of the Brassica rapa polysaccharide is 30,000-80,000, and the total polysaccharide content is 60-95 percent by weight. The Brassica rapa polysaccharide can be prepared into pharmaceutical acceptable formulations, such as granules, oral liquid, capsules, tablets, injections or powder. The Brassica rapa polysaccharide obtained by the method has available raw materials, simple method, low production cost, high polysaccharide content in the product, stable quality and easy control. The Brassica rapa polysaccharide has obvious anti-hypoxia activity. The Brassica rapa polysaccharide can be used for preparing the drugs or health care foods for prevention or / and treatment of low oxygen, hypoxia, hemic hypoxia, circulatory hypoxia and toxic hypoxia.

The invention relates to a pesticide, and especially relates to a complex bactericide composed of bacillus subtilis and a chemical bactericide. The bactericide is used for controlling brassica rapa pekinensis downy mildew. The bactericide comprises the chemical bactericide, bacillus subtilis XM16 CGMCC No.0372, and auxiliary materials. The bactericide is characterized in that the chemical bactericide in the active components is any one selected from metalaxyl, propamocarb, and mancozeb. A biocontrol bacterium is bacillus subtilis XM16, with the strain collection number of CGMCC No.0372. A weight ratio of metalaxyl to bacillus subtilis XM16 is 1-8:1-10. A weight ratio of propamocarb to bacillus subtilis XM16 is 1-10:15-140. A weight ratio of mancozeb to bacillus subtilis XM16 is 1-15:1-10. A form of the bactericide is usually hydrophilic powder or aqueous solution. The complex agent of the biocontrol bacterium and the chemical bactericide has a substantial synergetic effect in controlling brassica rapa pekinensis downy mildew. The biocontrol bacterium bacillus subtilis XM16 is advantaged in high bacteriostatic activity, high stress resistance, and wide bactericidal spectrum. The biocontrol bacterium is complexed with the oomycetes chemical bactericide, such that the pesticide dosage is reduced, and controlling effect is improved. The bactericide is an environment-friendly bactericide. The bactericide is advantaged in high biological safety, low cost, and substantial economic benefit.

The invention discloses oral liquid which is mainly prepared from Tibetan medicine-food dual-purpose herbal materials, and in particular relates to oral liquid for treating high altitude disease (HAD), which overcomes defects that most of the medicines for treating AHAD need to be taken a few days in advance and have no effect on acute high altitude disease (AHAD) in the prior art. The oral liquid is mainly prepared from brassica rapa linn. juice, hippophae thibetana juice, rheum emodi wall juice, rhodiola crenulata extracting solution and potentilla anserine extracting solution; and the medicine-food dual-purpose herbal materials fully exert efficacy of adjusting physiological functions of a human body by means of reasonable matching of a plurality of components so that the oral liquid has the function of rapidly and efficiently treating HAD. The invention further provides a method for preparing the oral liquid for treating AHAD. The oral liquid can be used for rapidly and effectively relieving adverse symptoms of AHAD and improving sign index without any adverse reaction or toxic or side effect; and the oral liquid is easy to produce and the preparation method is very simple.

The invention provides Brassica rapa plants and seeds thereof that are self-compatible, rapid-cycling and lack inbreeding depression. For instance, the invention provides plants and seeds of the Brassica rapa line designated B3. The invention thus relates to plants, seeds and tissue cultures of Brassica rapa plants that are self-compatible, rapid-cycling and lack inbreeding depression, such as Brassica rapa line B3, and methods to produce and propagate said plants by crossing such a Brassica rapa plant with itself, or another Brassica rapa plant. The invention further relates to seeds and plants produced by such crossing. Educational materials, such as a kit comprising said Brassica rapa plants are also provided by the invention.

The invention relates to pesticide filed, and particularly discloses a new application of a cocklebur fruit water extract in a weeding aspect, namely the cocklebur fruit water extract plays a complete inhibition role for brassica rapa L.Chinensis group under a certain concentration, and can be used as plant biological pesticides and weed killers.

The invention relates to a solid beverage and particularly relates to a brassica rapa L. extract composite solid beverage with a health function. The brassica rapa L. extract composite solid beverage comprises the following components in percentage by mass: 5%-50% of brassica rapa L. extract, 5%-20% of momordica grosvenori powder, 0.5%-3% of tea powder, 20%-85% of fructo-oligosaccharide, 0.1%-0.5% of stevioside and 0.2%-2% of essence. According to the brassica rapa L. extract composite solid beverage, the brassica rapa L. extract is successfully used in the composite solid beverage for the first time; and brassica rapa L. has a health function similar with rhodiola rosea, and can replace rhodiola rosea to a certain extent, so that the strong earthy smell of rhodiola rosea is made up and the development of a food industry is promoted.

The invention provides a pollen-specific promoter and application thereof. The promoter is derived from Brassica rapa and is an upstream sequence of a Delta8 sphingolipid desaturase gene. The promoter provided by the invention is a stable pollen-specific promoter having high specificity. The promoter can be used to promote the expression of various genes in pollen, and can be used for cultivatingmale sterile crop varieties and cultivating landscaping plants and the like with pollen eliminated or greatly reduced.

Seed coat color is a very important trait in oilseed type Brassica crops. Identification of the genes controlling the seed coat color is essential to the manipulation of these genes to develop new yellow-seeded germplasm for oilseed breeding The Brassica rapa Transparent Testa Glabra (TTG1) and Transparent Testa (TT1) genes may be used to control seed coat color in Brassica plants.

The present invention provides a composition comprising a green-yellow vegetable (e.g., broccoli, spinach, parsley, komatsuna (Brassica rapa L.), Japanese radish leaves) and a light-colored vegetable (e.g., lettuce, cabbage, celery), the composition having the following effects: (1) inhibiting the generation of blood lipid peroxide; (2) lowering blood TBARS levels or suppressing the elevation of blood TBARS levels; (3) increasing blood vitamin E levels; (4) enhancing blood antioxidant activity; (5) enhancing blood TRAP levels; (6) lowering blood active oxygen levels or suppressing the elevation of blood active oxygen levels; and (7) preventing or treating diabetic complications.

The invention belongs to the field of molecular markers and detection thereof and in particular relates to a method for identifying authenticity of a brassica rapa variety and a special SSR (simple sequence repeat) primer combination of the method. An SSR locus for identifying authenticity of a brassica rapa variety is obtained through data digging according to a reference genome Brassica_rapa V1.5 of brassica rapa and a reference genome Brassica olerecea V2.1 of brassica oleracea together with whole genome resequencing data of 40 genetic resources; the SSR primer combination is selected froma first SSR primer pair to a twenty-third SSR primer pair which are respectively applied to PCR (polymerase chain reaction) amplification on a first SSR locus to a twenty-third SSR locus, and are respectively optimally selected as nucleotide sequences which have 85-100% of homology with nucleotide sequences of SEQ ID NO:1-46 in a sequence table. The method can be applied to authenticity identification on the brassica rapa variety for a whole life cycle from seeds, and technical support is provided for protection on genetic resources and new varieties of brassica rapa.

The invention relates to the technical field of brassica and raphanus plant breeding, self-incompatible Chinese cabbages and self-incompatible radishes are taken as examples, when the Chinese cabbagesand the radishes enter an initial flowering stage and bloom in quantity, a potassium iodide solution of 1-10mM mixed with 0.025% Tween20 (for increasing the adhesiveness of the solution) is sprayed by a sprayer at 8: 00-10: 00 in sunny morning; the solution is sprayed on a cross-shaped stigma which is just unfolded until the surface of the stigma is stained with water drops. Spraying is performedfor the second time and the third time at an interval of 15 minutes. After spraying for the third time and the fog drops on the stigmas are completely dried, pollinating with fresh pollen, pinching off redundant unexpanded inflorescences, and sleeving with a seed collecting bag. The method is suitable for self-incompatible lines of brassica and raphanus plants. The potassium iodide solution is sprayed on stigmas, which are just developed into cross flowers, of inflorescences of self-incompatible Chinese cabbages (Brassica rapa L. Sp. Pekinensis) and self-incompatible radishes (Raphanus sativus) so that the self-affinity index can be increased to different degrees.

The invention discloses an overexpressing brassica napus and application of MYB43 of parental species of the overexpressing brassica napus in improving plant type and increasing yield. The gene familyof the MYB43 of brassica napus comprises BnMYB43-1, BnMYB43-2, BnMYB43-3 and BnMYB43-4 member genes; the parental specie brassica rapa has BrMYB43-1 and BrMYB43-2 member genes; the parental specie brassica oleracea has BoMYB43-1 and BoMYB43-2 member genes. After overexpression in plants, the roots of the plants become more developed and longer, the number of branch roots is more, the ratio of root to crown is increased, the plants are slightly higher, the stems become thicker, the number of effective branches per plant is increased, the number of effective pods per plant is increased, the yield of seeds per plant is increased, and the folding resistance and lodging resistance are also improved significantly. According to the overexpressing brassica napus and the application of the MYB43 of the parental species of the overexpressing brassica napus in improving the plant type and increasing the yield, a new method is provided for application of the MYB43 in improving the plant type, lodging resistance and seed yield of brassica napus, brassica rapa, brassica oleracea and other plants.

The invention belongs to the technical field of agricultural production, and particularly relates to a cold-resistant agent for brassica rapa L. The cold-resistant agent for the brassica rapa L. seedlings is mainly characterized by comprising raw materials as follows: 15-25 mg / L of ABA (abscisic acid) and the balance of water, wherein the purity of the ABA (abscisic acid) is higher than or equal to 99.9%. The cold-resistant agent aims to solve the problem that the brassica rapa L. in the northern cold and arid regions is vulnerable to low-temperature freezing damage, growth of the overground part and the root system of the brassica rapa L. is promoted with chemical control means, especially the thickening growth of roots, the outstanding performance refers to increase of dry matter accumulation, and safe wintering of the brassica rapa L. is guaranteed; meanwhile, permeability (relative electrical conductivity) of leaf cell membranes as well as accumulation of membrane lipid peroxidation malonaldehyde can be reduced, so that the low-temperature freezing degree of leaves of the brassica rapa L. is reduced, the cold resistance of the brassica rapa L. is remarkably improved, and the cold-resistant agent has great practical significance on brassica rapa L. production.

The invention discloses an InDelmolecular marker KB1 co-separated with a brassica rapa pekinensis clubroot resistance gene Crr1a, a primer and application of the molecular marker. The invention provides a method for identifying or auxiliary identifying whether brassica rapa pekinensis contains the clubroot resistance gene Crr1a or not. The method comprises the steps of adopting a brassica rapa pekinensis genome to be detected as a template; carrying out PCR (Polymerase Chain Reaction) amplification by the primer provided by the invention; according to the size of an amplified product, judgingwhether the brassica rapa pekinensis to be detected contains the clubroot resistance gene Crr1a or not and is the brassica rapa pekinensis with clubroot resistance or not. The molecular marker provided by the invention is utilized for auxiliary selecting the brassica rapa pekinensis with clubroot resistance, is high in accuracy, low in cost, and time and labor saving.

The invention relates to a method for preparing a natural pyrrole derivative, namely 4-(formyl-5-hydroxymethyl-1H-pyrrol-1-yl) butanoic acid, from a plant brassica rapa L. by extraction, purification and separation, belonging to the field of natural product extraction. Currently, all domestic and overseas methods for naturally extracting 4-(formyl-5-hydroxymethyl-1H-pyrrol-1-yl) butanoic acid have the defects of low yield, triviality and complexity, high cost and the like. The invention provides a method for obtaining 4-(formyl-5-hydroxymethyl-1H-pyrrol-1-yl) butanoic acid with the purity of greater than 98% from the plant brassica rapa L. by extraction, purification and separation. The method for preparing the natural pyrrole derivative from the plant brassica rapa L. by extraction, purification and separation is significant to the deep processing and the added value promotion of the brassica rapa L; and the extraction method is simple to operate, the yield and the purity of products are high, and the cost is low.

The present invention relates to a production method of a Brassica rapa L. anti-hypoxic fruit tea, and belongs to the technical field of food processing. To ease severe altitude sickness of tourists into the plateau and based on Tibetan medicine and modern medical research results, the Brassica rapa L. anti-hypoxic fruit tea is prepared by adopting Brassica rapa L., the plateau endemic anti-hypoxic plant, as a raw material and adding highland raw honey, fructose and cheese to achieve effects of alleviating altitude sickness and hypoxia resistance.

The invention relates to a production method of brassica rapa L. anti-hypoxia oral liquid, belonging to the technical field of food processing. In order to relieve serious altitude sickness of travelers when the travelers enter the plateau, the oral liquid for relieving altitude sickness is produced by taking brassica rapa L. which is an anti-hypoxia plant unique to Qinghai-Tibet Plateau as a raw material, and mixing the raw material with Tibetan yogurt naturally fermented in the Tibetan region, compound vitamin, a sweetening agent and drinking water according to the research results of Tibetan medicine and modern medicine.

The present invention relates to a method for extracting proteins and polysaccharides from brassica rapa. The method comprises the following steps: pretreatment is conducted: brassica rapa beating andultrasound treating are conducted to prepare slurry; enzymatic hydrolysis is conducted: snailase and iodoacetic acid are added to the slurry simultaneously and pH is also adjusted to be weakly acidic; heating is conducted, the weakly acidic slurry is slowly heated and then cooling to a normal temperature is conducted; centrifuging is conducted: the cooled slurry is centrifuged to obtain a clear solution; salting-out is conducted: then the pH of the clear solution is adjusted to be weakly acidic again, a mixed solution of a saturated solution of ammonium sulfate and a saturated solution of sodium sulfate is added at a suitable temperature, and even stirring and placing for layering are conducted; and protein and polysaccharide extraction is conducted: after the layering, precipitated liquid at a lower layer is subjected to multiple dialysis at a certain temperature to remove salts to obtain brassica rapa proteins, and a supernatant is dried to obtain brassica rapa polysaccharides. Themethod is a simple method to extract the proteins and polysaccharides from the brassica rapa simultaneously, besides, an average extraction rate of the proteins can reach 5.5% or more, and an averageextraction rate of the polysaccharides can reach 7.9% or more.

The invention relates to a method for extracting brassica rapa l. seed oil by using supercritical carbon dioxide, specially used for deep processing of brassica rapa l. seeds. Taking brassica rapa l. (Brassica rapa L.) seeds as the raw material, the method comprises the following process steps: the brassica rapa l. (Brassica rapa L.) seeds are treated by impurity removal, crushing and filtering, and is extracted by the supercritical carbon dioxide under the conditions that the extraction temperature is 46.10 DEG C, the extraction time is 80.40min and the extraction pressure is 31.65MPa, the brassica rapa l. seed oil extraction yield is 31.893-32.554 percent, and the purity of the brassica rapa l. seed oil is above 99 percent. A process for extracting the brassica rapa l. seed oil by using the supercritical carbon dioxide provides a new method for deep processing of the brassica rapa l. seeds, can efficiently carry out research on extracting the brassica rapa l. seed oil by the supercritical carbon dioxide, has the advantages of strong operability, favorable safety, higher yield, favorable repeatability, high extration yield, simple process, high product purity, non pollution and the like and can be used for extracting the brassica rapa l. seed oil in production.

The invention relates to a nutritional breakfast beneficial for working personnel to protect eyes, keep fit and strengthen the spleen and the stomach. Raw materials of the nutritional breakfast include 30 food materials in six categories of cereals, beans, fruits, vegetables, nuts and fibers. The concrete form of the nutritional breakfast is nutritional rice slices and fruit and vegetable particles. The invention also relates to a preparation method of the nutritional breakfast beneficial for the working personnel to protect the eyes, keep fit and strengthen the spleen and the stomach. The nutritional breakfast disclosed by the invention is comprehensive in nutrition, easy to digest and absorb and convenient to carry and brew; the effects of protecting the eyes and enhancing vision are achieved through addition of blueberries, strawberries, applies, grapes and carrots; the effects of keeping fit and slimming are achieved through addition of apples, carrots, lentinus edodes, pumpkins, oats, buckwheat and sweet potatoes; and the effect of nourishing the spleen and the stomach is achieved through addition of rice, millet, barley, triticum aestivum, semen nelumbinis, pisum sativum, bananas, broccoli and brassica rapa.

The present invention discloses a brassica rapa health-care product which is prepared by the following raw materials (parts by mass): 4-10 parts of brassica rapa, 3-8 parts of poria cocos, 3-8 parts of red dates, 2-6 parts of liquorice, 1-5 parts of honey powder and 1-5 parts of chrysanthemums. The present invention provides the brassica rapa health-care product whose raw materials are medicinal and edible food, are safe, have non-toxic side effects, have certain effects these materials themselves and certain local conditioning effects on human body, can be comprehensively used through reasonable matches, enhance the single-use effect, and fully prevent and treat phlegmatic hygrosis body constitution and other diseases caused by spleen deficiency. The brassica rapa health-care product can not only tonify spleen, remove dampness through diuresis and improve phlegmatic hygrosis body constitution, but also comprehensively regulate internal organs of the body, and it is worth mentioning that long-term use of the product also can prolong life.

The invention relates to the technical field of application of neutral polysaccharide from Brassica rapa L., and particularly discloses application of the neutral polysaccharide from Brassica rapa L.in preparation of antioxidant drugs. Indexes in serum and whole brain and liver homogenate of mice in a D-galactose-induced aging mouse model and a control group are determined, respectively. It is found that the neutral polysaccharide from Brassica rapa L. has an antioxidant effect on the D-galactose-induced aging mouse model. The invention finds, for the first time, that the neutral polysaccharide from Brassica rapa L. significantly increases the activity of SOD, T-AOC, CAT, GSH and GSH-Px in serum and whole brain and liver homogenate, and significantly reduces the MDA content, thereby improving antioxidant ability and delaying senescence.

The invention relates to the technical field of Brassica rapa powder preparation, and specifically relates to a preparation method of Brassica rapa powder. The method comprises the following steps: S1, cutting fresh Brassica rapa into pieces, making the solid-liquid ratio of Brassica rapa pieces to water be 1:1 to 1:5, putting the mixture of Brassica rapa pieces and water in a tissue stamp mill for pulping to get Brassica rapa pulp; S2, pouring the Brassica rapa pulp into a colloid mill for colloid milling to get colloid-milled Brassica rapa pulp; and S3, adding anti-caking agent to the colloid-milled Brassica rapa pulp to get anti-caking Brassica rapa pulp. The invention discloses a preparation method of Brassica rapa powder. The Brassica rapa powder prepared by the method is yellow, has uniform particles, is non-caking and fragrant, and tastes of Brassica rapa. The Brassica rapa powder is easy to eat, and can be eaten directly after being mixed with water. Moreover, the Brassica rapa powder can be preserved at room temperature for a long time, so that the supply period of the product is prolonged, and the production and sale peaks can be balanced.

The invention discloses high-calorific-value environment-friendly fuel and a preparation method thereof. The high-calorific-value environment-friendly fuel is prepared from the following raw materials in parts by weight: 20-30 parts of brassica rapa straw powder, 10-20 parts of rapeseed straw powder, 20-30 parts of hemp stem powder, 10-20 parts of sunflower straw powder, 30-40 parts of biomass block fuel, 1-3 parts of turpentine, 1-3 parts of ethylene glycol, 0.1-0.3 part of an antioxidant, 1-1.5 parts of an additive, 1-2 parts of a bio-enzyme additive and 3-5 parts of an additive. The high-calorific-value environment-friendly fuel is high in combustion heat and is environment-friendly; the purpose of waste resource utilization can be achieved; moreover, due to fermentation treatment in an oxygen-free environment, tissue structures of biomass raw materials can be improved in compactness.

The present invention discloses ginseng and semen cassiae tableted candy and a preparation method thereof. The product, namely the ginseng and semen cassiae tableted candy is prepared from the following raw materials: 10%-20% of ginsengs, 5%-10% of semen cassiae, 10%-20% of brassica rapa var. rapa, 25%-35% of brown sugar, 2%-10% of isomaltooligosaccharide, 5%-10% of hydroxypropyl starch, 10%-15% of cocoa powder and 1%-2% of magnesium stearate. The product is prepared by the following processing processes of raw and accessory material fine selecting, mixing, crushing, granulating, drying, granule separating, total mixing, tableting, sterilizing, packaging, etc. The tableted candy is rich in smell and comfortable in taste, has effects of clearing heat and relieving toxins, supplementing qi and strengthening the spleen, and moistening intestines and relaxing bowels, has advantages of being safe and effective, small in toxic and side effects, long in a storage life, easy to carry and eat, etc., and is simple in preparation method and conducive to industrial productions.

The invention relates to application of brassica rapa organic acid extracted from brassica rapa plants to the preparation of medicaments for reducing blood sugar. The brassica rapa organic acid has an obvious effect in aspects of reducing blood sugar, adopts readily available raw materials, and is low in production cost, high in content, stable in quality and easy to control, and a method is simple.

The invention discloses a locus specific co-dominant ASM (allele specific marker) directly related to identification of Brassica rapa L.ssp pekinensis eIF (iso) 4E.a wild type and mutant. The marker related to wild type locus detection is named as ASM-iso4E.a, has fragment size of 1636bp, and is shown as a SEQ ID No.1. The corresponding mutation locus detection related marker is named as ASM-iso4e.a, has fragment size of 640bp, and is shown as a SEQ ID No.2. In the invention, by making use of a homology based cloning technology, a mutant of the eIF (iso) 4E.a locus is discovered, and the molecular marker for identifying the locus is developed. The marker can be utilized for accurate selection of the genotype of backcrossing transformation progenies. Meanwhile, the marker also can be used for screening Brassica rapa L.ssp pekinensis germplasm resources so as to seek mutant materials with more abundant genetic backgrounds.

The invention discloses externally applied medicine for treating leucoderma, which comprises the following active ingredients by weight: 10 g musk, 25 g radix angelicae, 35 g vines of multiflower knotweed, 24 g carthamus tinctorius, 25 g cantharides, 55 g dittany bark, 25 g brassica rapa, 18 g radix paeoniae rubra, 55 g radix saposhnikoviae, 20 g croton, 24 g myrrh and 18 g campsis grandiflora. The liquid medicine has a short treat period for the leucoderma, takes effect rapidly, and can take effect when a patient takes the medicine for approximately 10 days generally. The leucoderma can be cured within a month, and the cure rate for various patients can be over 85%.