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Method for extracting proteins and polysaccharides from brassica rapa

An extraction method and protein technology, applied in the field of protein and polysaccharide extraction, can solve the problems of high polysaccharide content and unmanned research

Inactive Publication Date: 2019-04-12
河北中科汉禧生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been found through research that turmeric is high in protein and polysaccharide, and it contains a variety of effective ingredients that inhibit thyroxine synthesis and anti-distortion, but no one has studied the method of efficiently extracting these two ingredients at the same time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation method for simultaneously extracting protein and polysaccharide from mandarin greens provided by the present embodiment comprises the following steps:

[0023] (1) Pretreatment of the turmeric: after cleaning the vine with clean water, add water 5 times the weight of the vine, and perform beating treatment by mechanical method; and then ultrasonicate for 10 minutes under the condition of 20 Hz.

[0024] (2) Enzymolysis: add helicase with a mass ratio of 1:300 to the mandarin greens in the slurry, and simultaneously add iodoacetic acid with a mass ratio of 1:1000 to the mandarin greens, and adjust the pH to 5.

[0025] (3) Heat treatment: Pour the enzymatically hydrolyzed broccoli slurry into a container and heat for 30 minutes, set the temperature at 40° C., and cool for 12 hours after heating.

[0026] (4) Centrifugal treatment: put the heat-treated turmeric slurry mixture into a high-speed refrigerated centrifuge and centrifuge for 20 minutes with a ce...

Embodiment 2

[0031] The method for simultaneously extracting protein and polysaccharide from mandarin greens provided by the present embodiment comprises the following steps:

[0032] (1) Pretreatment of the turmeric: after cleaning the vine with clean water, add water 8 times the weight of the vine, and perform beating treatment by mechanical method; and then ultrasonicate for 15 minutes under the condition of 30 Hz.

[0033] (2) Enzymolysis: Add helicase with a mass ratio of 1:500 to the mandarin greens in the slurry, and simultaneously add iodoacetic acid with a mass ratio of 1:1000 to the mandarin greens to adjust the pH to 7.

[0034] (3) Heat treatment: Pour the enzymatically hydrolyzed broccoli slurry into a container and heat for 40 minutes, set the temperature at 50° C., and cool for 12 hours after heating.

[0035] (4) Centrifugal treatment: put the heat-treated turmeric slurry mixture into a high-speed refrigerated centrifuge and centrifuge for 20 minutes with a centrifugal forc...

Embodiment 3

[0040] The method for simultaneously extracting protein and polysaccharide from mandarin greens provided by the present embodiment comprises the following steps:

[0041] (1) Pretreatment of the turmeric: after cleaning the vine with clean water, add water 9 times the weight of the vine, and perform beating treatment by mechanical method; then, ultrasonicate for 15 minutes under the condition of 40 Hz.

[0042] (2) Enzymolysis: Add helicase with a mass ratio of 1:500 to the mandarin greens in the slurry, and simultaneously add iodoacetic acid with a mass ratio of 1:1000 to the mandarin greens to adjust the pH to 7.

[0043] (3) Heat treatment: Pour the enzymatically hydrolyzed broccoli slurry into a container and heat for 50 minutes, set the temperature at 60° C., and cool for 12 hours after heating.

[0044] (4) Centrifugal treatment: put the heat-treated turmeric slurry mixture into a high-speed refrigerated centrifuge and centrifuge for 25 minutes with a centrifugal force o...

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PUM

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Abstract

The present invention relates to a method for extracting proteins and polysaccharides from brassica rapa. The method comprises the following steps: pretreatment is conducted: brassica rapa beating andultrasound treating are conducted to prepare slurry; enzymatic hydrolysis is conducted: snailase and iodoacetic acid are added to the slurry simultaneously and pH is also adjusted to be weakly acidic; heating is conducted, the weakly acidic slurry is slowly heated and then cooling to a normal temperature is conducted; centrifuging is conducted: the cooled slurry is centrifuged to obtain a clear solution; salting-out is conducted: then the pH of the clear solution is adjusted to be weakly acidic again, a mixed solution of a saturated solution of ammonium sulfate and a saturated solution of sodium sulfate is added at a suitable temperature, and even stirring and placing for layering are conducted; and protein and polysaccharide extraction is conducted: after the layering, precipitated liquid at a lower layer is subjected to multiple dialysis at a certain temperature to remove salts to obtain brassica rapa proteins, and a supernatant is dried to obtain brassica rapa polysaccharides. Themethod is a simple method to extract the proteins and polysaccharides from the brassica rapa simultaneously, besides, an average extraction rate of the proteins can reach 5.5% or more, and an averageextraction rate of the polysaccharides can reach 7.9% or more.

Description

technical field [0001] The invention belongs to the technical field of biological material extraction, in particular to a method for extracting proteins and polysaccharides from mandarin greens. Background technique [0002] Manjing is the root or leaf of the Brassica plant Brassica (Brassicarapa L.), also known as turnip, which is a common edible vegetable. It can eliminate food and lower qi, detoxify and reduce swelling. Modern research has found that turmeric contains protein, polysaccharide, fat, calcium, phosphorus, riboflavin, niacin, vitamins, and organic acid compounds such as caffeic acid and ferulic acid. It has antibacterial, antiparasitic, and antithyroid properties. Vitamin synthesis, anti-distortion, anti-aging and other effects. There are many kinds of Manjing, and the components of Manjing produced in various places are often not the same. Although there are many types of active ingredients in Manjing, they have not been fully utilized, and there is a lack ...

Claims

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Application Information

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IPC IPC(8): C07K1/14C08B37/00
CPCC07K1/145C08B37/00
Inventor 康跻耀贺佩孔英俊张贵锋
Owner 河北中科汉禧生物科技有限公司
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