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Reagent for overcoming self-incompatibility of brassica and raphanus and use method and application of reagent

A technology of the genus Radish and Brassica, applied in the field of breeding, can solve problems such as unfavorable promotion

Active Publication Date: 2020-12-08
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But these operations are not conducive to promoting

Method used

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  • Reagent for overcoming self-incompatibility of brassica and raphanus and use method and application of reagent
  • Reagent for overcoming self-incompatibility of brassica and raphanus and use method and application of reagent
  • Reagent for overcoming self-incompatibility of brassica and raphanus and use method and application of reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Take Chinese cabbage as an example

[0029] For Chinese cabbage, remove the flowers that have opened one day before spraying potassium iodide, and collect the seed bags. Before spraying, choose a black sunshade net with good shading effect and high mesh number to cover the top and surroundings of the plants with brackets or pull nets, etc., to create a weak light environment around the plants to avoid weakening due to the easy decomposition of potassium iodide when exposed to light. spray effect. Carry out potassium iodide solution spraying (potassium iodide solution solution contains 0.025% Tween20, potassium iodide concentration is 0 mM, 1 mM, 5 mM, 10 mM) to new inflorescence, each spraying until can see that there are drops of water on the stigma, totally Spray 3 times with an interval of 15 minutes. After the third spray, wait for the stigma to dry before self-pollinating, and collect the seed belts to prevent contamination of other pollen. After 20...

Embodiment 2

[0035] Embodiment 2: Taking radish as an example

[0036] The day before the radish is sprayed with potassium iodide, the flowers that have already opened are removed, and the seed bags are collected. Before spraying, choose a black sunshade net with good shading effect and high mesh number to cover the top and surroundings of the plants with brackets or pull nets, etc., to create a weak light environment around the plants to avoid weakening due to the easy decomposition of potassium iodide when exposed to light. spray effect. Spray potassium iodide solution on new inflorescences (potassium iodide solution contains 0.025% Tween20, potassium iodide concentration is 0 mM, 0.5 mM, 1 mM, 5 mM), spray each time until water droplets can be seen on the stigma, and spray Apply 3 times with an interval of 15 minutes. After the third spraying, wait for the stigma to dry before self-pollinating, and cover the seed belt to prevent contamination of other pollen. After 20 days, the fruit ...

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Abstract

The invention relates to the technical field of brassica and raphanus plant breeding, self-incompatible Chinese cabbages and self-incompatible radishes are taken as examples, when the Chinese cabbagesand the radishes enter an initial flowering stage and bloom in quantity, a potassium iodide solution of 1-10mM mixed with 0.025% Tween20 (for increasing the adhesiveness of the solution) is sprayed by a sprayer at 8: 00-10: 00 in sunny morning; the solution is sprayed on a cross-shaped stigma which is just unfolded until the surface of the stigma is stained with water drops. Spraying is performedfor the second time and the third time at an interval of 15 minutes. After spraying for the third time and the fog drops on the stigmas are completely dried, pollinating with fresh pollen, pinching off redundant unexpanded inflorescences, and sleeving with a seed collecting bag. The method is suitable for self-incompatible lines of brassica and raphanus plants. The potassium iodide solution is sprayed on stigmas, which are just developed into cross flowers, of inflorescences of self-incompatible Chinese cabbages (Brassica rapa L. Sp. Pekinensis) and self-incompatible radishes (Raphanus sativus) so that the self-affinity index can be increased to different degrees.

Description

technical field [0001] The invention belongs to the technical field of breeding, and more specifically relates to a reagent for overcoming the self-incompatibility between plants of the genus Brassica and the genus Radish, as well as its usage and application. Background technique [0002] Self-incompatibility is a genetic mechanism evolved by angiosperms to prevent self-pollination and promote cross-pollination, which plays an important role in preventing self-depression and promoting offspring fitness. Generally speaking, self-incompatibility can be divided into sporophytic self-incompatibility and gametophytic self-incompatibility. Brassica vegetable crops such as Chinese cabbage and Radish plants such as radish are typical sporophyte self-incompatibility. The tightly linked S-gene, that is, the SP11 / SCR signal molecule expressed by the anther tapetum carried by pollen and SRK expressed by stigma-mastoid cells completes self-pollen recognition. During self-pollination, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N59/12A01P21/00A01H1/02
CPCA01H1/02A01N59/12
Inventor 段巧红黄家保原玉香魏小春王茂帅张丽丽
Owner SHANDONG AGRICULTURAL UNIVERSITY
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