The invention discloses a preparation method of Hericium erinaceus coffee, comprising the following steps: slicing fresh mature Hericium erinaceus, baking and crushing processing. The technology is simple, and the product has rich nutritive value, mellow and fragrant taste and has fresh mouthfeel. health-care value of Hericium erinaceus is fully reflected. The Hericium erinaceus coffee can achieve effects of protecting liver, raising immunity, reducing blood sugar, preventing arteriosclerosis and arteriosclerosis complication, preventing hypertension, heart disease and heart failure, resisting senility, preventing cancer, improving human memory, improving the function of digestive system and the like, is suitable for any people, and is convenient to carry. In addition, mouthfeel and fragrance of coffee will not influenced, consumption of coffee beans is reduced, and the side effect of long-term use of plain coffee is overcome. The product will become a mainstream product of human healthy drinks in the future.