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Reagent for overcoming self-incompatibility between Brassica and Radish and its usage and application

A technology of the genus Radish and Brassica, applied in the field of breeding, can solve the problems of unfavorable promotion, etc., and achieve the effects of less plant damage, reduced active oxygen, and significant effects

Active Publication Date: 2021-05-25
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But these operations are not conducive to promoting

Method used

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  • Reagent for overcoming self-incompatibility between Brassica and Radish and its usage and application
  • Reagent for overcoming self-incompatibility between Brassica and Radish and its usage and application
  • Reagent for overcoming self-incompatibility between Brassica and Radish and its usage and application

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1: Take Chinese cabbage as an example

[0029] For Chinese cabbage, remove the flowers that have opened one day before spraying potassium iodide, and collect the seed bags. Before spraying, choose a black sunshade net with good shading effect and high mesh number to cover the top and surroundings of the plants with brackets or pull nets, etc., to create a weak light environment around the plants to avoid weakening due to the easy decomposition of potassium iodide when exposed to light. spray effect. Carry out potassium iodide solution spraying (potassium iodide solution solution contains 0.025% Tween20, potassium iodide concentration is 0 mM, 1 mM, 5 mM, 10 mM) to new inflorescence, each spraying until can see that there are drops of water on the stigma, totally Spray 3 times with an interval of 15 minutes. After the third spray, wait for the stigma to dry before self-pollinating, and collect the seed belts to prevent contamination of other pollen. After 20...

Embodiment 2

[0035] Embodiment 2: Taking radish as an example

[0036] The day before the radish is sprayed with potassium iodide, the flowers that have already opened are removed, and the seed bags are collected. Before spraying, choose a black sunshade net with good shading effect and high mesh number to cover the top and surroundings of the plants with brackets or pull nets, etc., to create a weak light environment around the plants to avoid weakening due to the easy decomposition of potassium iodide when exposed to light. spray effect. Spray potassium iodide solution on new inflorescences (potassium iodide solution contains 0.025% Tween20, potassium iodide concentration is 0 mM, 0.5 mM, 1 mM, 5 mM), spray each time until water droplets can be seen on the stigma, and spray Apply 3 times with an interval of 15 minutes. After the third spraying, wait for the stigma to dry before self-pollinating, and cover the seed belt to prevent contamination of other pollen. After 20 days, the fruit ...

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Abstract

The invention relates to the technical field of plant breeding of the genus Brassica and the genus Radish. Taking self-incompatible Chinese cabbage and self-incompatible radish as examples, when the Chinese cabbage and radish enter the initial flowering stage and bloom in large quantities, the radishes are planted at 8 am on a sunny day. : 00-10:00 time period, 1 mM-10 mM potassium iodide solution mixed with 0.025% Tween-20 (increasing the adhesion of the solution), using a sprayer, sprayed on the stigma that has just expanded into a cross, until the stigma Water beads on the surface. Spray the second and third times at intervals of 15 minutes. After spraying for the third time, the mist droplets on the stigma are all dry, and then fresh pollen is given, and the excess unexpanded inflorescences are pinched off, and the seed collection bag is put on. The method of the invention is applicable to the self-incompatibility lines of plants of the genus Brassica and the genus Radish. To self-incompatibility Chinese cabbage ( Brassica rapa L. ssp. pekinensis ) and self-incompatible radish ( Raphanus sativus ) Potassium iodide solution was sprayed on the stigma of the cruciferous inflorescence just unfolded, which can improve the self-compatibility index to varying degrees.

Description

technical field [0001] The invention belongs to the technical field of breeding, and more specifically relates to a reagent for overcoming the self-incompatibility between plants of the genus Brassica and the genus Radish, as well as its usage and application. Background technique [0002] Self-incompatibility is a genetic mechanism evolved by angiosperms to prevent self-pollination and promote cross-pollination, which plays an important role in preventing self-depression and promoting offspring fitness. Generally speaking, self-incompatibility can be divided into sporophytic self-incompatibility and gametophytic self-incompatibility. Brassica vegetable crops such as Chinese cabbage and Radish plants such as radish are typical sporophyte self-incompatibility. The tightly linked S-gene, that is, the SP11 / SCR signal molecule expressed by the anther tapetum carried by pollen and SRK expressed by stigma-mastoid cells completes self-pollen recognition. During self-pollination, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N59/12A01P21/00A01H1/02
CPCA01H1/02A01N59/12
Inventor 段巧红黄家保原玉香魏小春王茂帅张丽丽
Owner SHANDONG AGRICULTURAL UNIVERSITY
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