The invention belongs to the field of innovation and research of
plant genetics breeding and apple
germplasm, in particular to an SSR (simple
sequence repeat)
molecular marker III for identifying descendant plants of Gala apple and application thereof. The SSR
molecular marker III is characterized in that in the detection process, the SSR molecular markers linked with No.4, No.6, No.12 and No.14 chromosomes are simultaneously used,
DNA (deoxyribonucleic acid) of an apple
gene group is used as a PCR (
polymerase chain reaction) amplification template, the SSR molecular markers are respectively used as primer pairs, and the PCR amplification and product detection are performed, so as to identify the
genetics linkage, anther culture plants and variety sources. The SSR
molecular marker III has the advantages that the SSR molecular marker is used for identifying the apple linkage group of the identifying material for the first time, and the SSR molecular markers linked with No.4, No.6, No.12 and No.14 chromosomes of the apple are disclosed; the molecular marker is a co-dominant marker, so that the Gala apple
genetic linkage group, the anther culture
plant and the Gala source
plant are quickly and accurately identified; the
molecular level support is provided for further accelerating the utilization of the Gala apple and important agronomic trait linkage
gene, and the genetic breeding of apple homozygous plants.