36 results about "Simple Repetitive Sequences" patented technology

the invention discloses simple repeating sequence primer of three-wart swimming crab, which comprises the following parts: CAC ACA CAC ACA CAC AG,AGA GAG AGA GAG AGA GYT,AGA GAG AGA GAG AGA GYC,AGA GAG AGA GAG AGA GYA,GAG AGA GAG AGA GAG AYT,CAC ACA CAC ACA CAC ARC,CAC ACA CAC ACA CAC ARG, GTG TGT GTG TGT GTG TYA,GTG TGT GTG TGT GTG TYC,GTG TGT GTG TGT GTG TYG.

The invention belongs to the technical field of plant biotechnology, and specifically discloses a method for rapidly identifying chromosome ploidy of an avena plant. An SSR (Simple Sequence Repeats) molecule is utilized to mark characteristics that avena plants with different ploidy have different sizes and copy numbers, and the chromosome ploidy of the avena plants can be identified rapidly by combining a PCR amplification technology and a gel electrophoresis technology by virtue of different numbers of amplified product segments. The method for rapidly identifying chromosome ploidy of the avena plant has the advantage that ploidy of the plant can be identified rapidly under the condition that the ploidy of the avena plan is not known in advance, and the method is applicable to avena plants.

the invention discloses simple repeating sequence primer of three-wart swimming crab, which comprises the following parts: CAC ACA CAC ACA CAC AG,AGA GAG AGA GAG AGA GYT,AGA GAG AGA GAG AGA GYC,AGA GAG AGA GAG AGA GYA,GAG AGA GAG AGA GAG AYT,CAC ACA CAC ACA CAC ARC,CAC ACA CAC ACA CAC ARG, GTG TGT GTG TGT GTG TYA,GTG TGT GTG TGT GTG TYC,GTG TGT GTG TGT GTG TYG.

The invention discloses an exploring method for a biological genome simple repeat sequence, which is characterized by comprising the following steps of: forming a regular expression according to the characteristics of the biological genome SSR (Simple Repeat Sequence) needed to be explored; analyzing a to-be-analyzed sequence according to the regular expression, judging whether the to-be-analyzed sequence contains a target SSR meeting the requirement of the regular expression, if so, outputting the target SSR; if not, displaying information about that the to-be-analyzed sequence contains no target SSR. Thus, the exploring method and the exploring device for the biological genome simple repeat sequence cannot generate redundant result in a SSR exploring process, so that the configuration complexity of the SSR exploring process is reduced, SSR exploring efficiency is improved and difficulty in development of SSR exploring software is reduced.

The invention discloses a primer pair, a castanopsis hystrix SSR1 (Simple Sequence Repeat 1) marker and a preparation method and application thereof. According to the preparation method, the total DNA (Deoxyribonucleic Acid) of castanopsis hystrix is subjected to PCR (Polymerase Chain Reaction) amplification by using the specific primer pair HZ1, thereby obtaining the SSR1 marker. The method is simple and feasible and is simple and convenient in operation; shown by tests which are carried out in members of two natural castanopsis hystrix populations and among the members, the obtained SSR has relatively high polymorphism. The castanopsis hystrix SSR1 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to the genetic diversity evaluation of castanopsis hystrix, the genetic map construction of castanopsis hystrix, the research on the spatial distribution pattern of the genetic variation of castanopsis hystrix population and the origination and evolution of castanopsis hystrix and the standardization of target genes, and can also be applied to the molecular marker assisted breeding and QTL (Quantitative Trait Locus) research of castanopsis hystrix.

The invention discloses a primer pair, a castanopsis hystrix SSR5 (Simple Sequence Repeat 5) marker and a preparation method and application thereof. According to the preparation method, the total DNA (Deoxyribonucleic Acid) of castanopsis hystrix is subjected to PCR (Polymerase Chain Reaction) amplification by using the specific primer pair HZ5, thereby obtaining the SSR5 marker. The method is simple and feasible and is simple and convenient in operation; shown by tests which are carried out in members of two natural castanopsis hystrix populations and among the members, the obtained SSR has relatively high polymorphism. The castanopsis hystrix SSR5 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to the genetic diversity evaluation of castanopsis hystrix, the genetic map construction of castanopsis hystrix, the research on the spatial distribution pattern of the genetic variation of castanopsis hystrix population and the origination and evolution of castanopsis hystrix and the standardization of target genes, and can also be applied to the molecular marker assisted breeding and QTL (Quantitative Trait Locus) research of castanopsis hystrix.

The invention discloses hibiscus cannabinus L. drought response gene EST-SSR primers and a kit. 11 pairs of primers are provided, and one pair or multiple pairs of primers are used as EST-SSR polymorphism markers for identifying the genetic relationship of hibiscus cannabinus L., the genetic diversity or the differences of simple repeat sequence polymorphisms in specific exon regions of drought-related genes. The hibiscus cannabinus L. drought response gene EST-SSR primers and the kit disclosed by the invention have the benefits that a good marker reserve is provided for molecular marker-assisted breeding of the hibiscus cannabinus L., and a candidate molecular marker for detection is also provided for functional allelic variation of key genes screened in the natural variation of the hibiscus cannabinus L.

The invention discloses a Beech tree SSR1 marker, a primer pair and a preparation method and application thereof. The inventor uses a specific primer pair JS1 to amplify the total DNA of the beech tree by PCR to obtain a simple repeat sequence SSR1 marker. The method is simple and easy to operate, and the obtained simple repeat sequences have high polymorphisms in the population and between populations of the three natural populations of Zelkova. Beech tree SSR1 marker is a co-dominant marker. Compared with other molecular markers, SSR marker has good repeatability and high reliability. It can be applied to the evaluation of genetic diversity of beech tree, the construction of genetic map of beech tree, and the spatial distribution of genetic variation of beech tree population. The study of pattern and origin and evolution of Beech trees, as well as the calibration of target genes, can also be applied to molecular marker-assisted breeding and QTL research of Beech trees.

The invention discloses a beech SSR2 marker, a primer pair, its preparation method and application. The inventor uses a specific primer pair JS2 to perform PCR amplification on the beech total DNA to obtain a simple repeat sequence SSR2 marker. The method is simple and easy to operate, and the obtained simple repeat sequence has high polymorphism detected in the population and between populations of three natural populations of beech. The beech SSR2 marker is a co-dominant marker. Compared with other molecular markers, the SSR marker has good repeatability and high reliability. It can be applied to the evaluation of genetic diversity of beech, the construction of beech genetic map, and the spatial distribution of genetic variation in beech populations. The research on pattern and origin and evolution of beech, as well as the calibration of target genes, can also be applied to molecular marker-assisted breeding and QTL research of beech.

The invention discloses a high-throughput typing technique universal to various molecular markers and belongs to the field of molecular biology. By the use of hybrid template production of 5' terminal biotin labeling, a target area having single-nucleotide polymorphism, simple sequence repeat, insertion deletion, copy number variation and the like and containing various molecular markers and target areas such as exon and species specific single-copy areas can be subjected to capturing and gene typing; meanwhile, allele quantitative typing and precise quantitative detecting are both considered; site selection flexibility of the solution hybridization is combined with the advantages of the high-throughput sequencing technique, such as high throughput and low cost. Compared with the use of the existing molecular marker typing technique, the use of the high-throughput typing technique solves the problems such that the existing fixed-point molecular marker typing technique is available for only typing single molecular markers and unavailable for typing various markers at the same time or precisely and quantitatively detecting alleles, and production of random markers or unlabeled hybrid templates is limited.

The invention relates to a simple repetitive sequence primer for Rainbow Moerella, which is characterized in that the simple repetitive sequence primer for the Rainbow Moerella is a primer group which is formed by 10 Rainbow Moerella primers. The screened amplified products of the primer are clear and stable, have good polymorphism, can be applied in idioplasm evaluation and idioplasm identification of the Rainbow Moerella, and diversity evaluation of colonies and remains of the Rainbow Moerella, and have the characteristics of quickness, economy, accuracy, simple and convenient operation andso on.

The invention discloses a primer pair, a castanopsis hystrix SSR2 (Simple Sequence Repeat 2) marker and a preparation method and application thereof. According to the preparation method, the total DNA (Deoxyribonucleic Acid) of castanopsis hystrix is subjected to PCR (Polymerase Chain Reaction) amplification by using the specific primer pair HZ2, thereby obtaining the SSR2 marker. The method is simple and feasible and is simple and convenient in operation; shown by tests which are carried out in members of two natural castanopsis hystrix populations and among the members, the obtained SSR has relatively high polymorphism. The castanopsis hystrix SSR2 marker is a co-dominant marker; compared with other molecular markers, the SSR marker is good in repeatability and high in reliability, can be applied to the genetic diversity evaluation of castanopsis hystrix, the genetic map construction of castanopsis hystrix, the research on the spatial distribution pattern of the genetic variation of castanopsis hystrix population and the origination and evolution of castanopsis hystrix and the standardization of target genes, and can also be applied to the molecular marker assisted breeding and QTL (Quantitative Trait Locus) research of castanopsis hystrix.

The invention relates to a method for detecting redried tobacco varietal complexity. According to the technical scheme, the method comprises the following steps of 1, extraction of redried tobacoo DNA; 2, synthesis of primers; 3, a simple repetition inter-sequence polymorphism-polymerization enzyme-linked reaction, wherein the ISSR primer 826 or the ISSR primer 856 is subjected to an ISSR-PCR in redried tobacco to be detected; 4, comparison of a standard diagram, wherein strip comparison is performed on a standard fingerprint chromatogram and an imaging picture obtained after electrophoresis of the redried tobacco ISSR-PCR to be detected is performed, if strips can be matched, the refried tobacco is in one corresponding variety, and if the strips cannot be matched, the refried tobacco is in the mixed variety, and detection is completed. The method has the advantages that the method is provided for authentication and detection of redried tobacco, the primers obtained through screening of the experiment can be directly referred to in the redried tobacco experiment process and subjected to an ISSR-PCR directly at the corresponding annealing temperature, and then the fingerprint spectrum in the corresponding variety is obtained to be used as a follow-up comparison standard spectral band.