Method for detecting redried tobacco varietal complexity

Abstract

The invention relates to a method for detecting redried tobacco varietal complexity. According to the technical scheme, the method comprises the following steps of 1, extraction of redried tobacoo DNA; 2, synthesis of primers; 3, a simple repetition inter-sequence polymorphism-polymerization enzyme-linked reaction, wherein the ISSR primer 826 or the ISSR primer 856 is subjected to an ISSR-PCR in redried tobacco to be detected; 4, comparison of a standard diagram, wherein strip comparison is performed on a standard fingerprint chromatogram and an imaging picture obtained after electrophoresis of the redried tobacco ISSR-PCR to be detected is performed, if strips can be matched, the refried tobacco is in one corresponding variety, and if the strips cannot be matched, the refried tobacco is in the mixed variety, and detection is completed. The method has the advantages that the method is provided for authentication and detection of redried tobacco, the primers obtained through screening of the experiment can be directly referred to in the redried tobacco experiment process and subjected to an ISSR-PCR directly at the corresponding annealing temperature, and then the fingerprint spectrum in the corresponding variety is obtained to be used as a follow-up comparison standard spectral band.

Description

technical field [0001] The invention relates to a detection method of recured tobacco, in particular to a method for detecting mixed varieties of recured tobacco. Background technique [0002] Flue-cured tobacco is the raw material after the first processing of plants, and re-cured tobacco is the preparatory process of sorting and processing tobacco leaves from agricultural products into industrial raw materials. It is another drying after tobacco leaves are prepared, so it is called re-cured. During the re-curing process of tobacco leaves, moldy and unqualified tobacco leaves should be removed to ensure the quality of re-cured tobacco. The pests of the tobacco leaves can be killed while heating and drying, and the moisture content of the rebaked tobacco leaves is very low, which is good for long-term storage, alcoholization or artificial fermentation. Leaf threshing and redrying is a key link in providing high-quality raw materials for cigarette companies. At present, tob...

AI Technical Summary

Problems solved by technology

So far, the identification of differences among tobacco leaf varieties has only been evaluated based on the appearance quality of tobacco leaves. In the process of tobacco leaf production, purchase, rebaking, and storage, a scientific tracking and monitoring system has not been formed, resulting in mixed varieties, wrong The phenomenon of standardization has seriously affected the stability of the quality and style of cigarettes produced in Yunzhong, such as: Red-flowered Jinyuan, Yun87, NC297, NC102, K326, etc.

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