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InDel molecular marker realizing coseparation with rice xanthomonas oryzae resistant gene xa34(t) as well as detection primers and applications of InDel molecular marker

A technology of xa34-nv7indelrv and rice bacterial blight, applied in the field of agricultural biology, can solve the problems of low throughput, long time-consuming, expensive reagents, etc., and achieve the effects of high specificity, reduced pollution, and long detection time

Active Publication Date: 2018-06-01
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This detection method requires the use of toxic and harmful chemical reagents such as acrylamide and formaldehyde, and the reagents required for silver staining are also expensive
Moreover, the detection method has the disadvantages of long time consumption and low throughput.
The above problems have become the bottleneck restricting the wide application of xa34(t) gene in the field of rice disease resistance molecular breeding

Method used

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  • InDel molecular marker realizing coseparation with rice xanthomonas oryzae resistant gene xa34(t) as well as detection primers and applications of InDel molecular marker
  • InDel molecular marker realizing coseparation with rice xanthomonas oryzae resistant gene xa34(t) as well as detection primers and applications of InDel molecular marker
  • InDel molecular marker realizing coseparation with rice xanthomonas oryzae resistant gene xa34(t) as well as detection primers and applications of InDel molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Development of InDel molecular marker co-segregated with xa34(t) gene and verification of the marker in main rice varieties planted in South China and main rice bacterial blight resistant resource varieties.

[0037] (1) On the basis of the results of fine mapping of the xa34(t) gene, within the range between the RM10927 and BGID25 markers on chromosome 1, the resistant variety BG1222 of the xa34(t) gene and the susceptible control variety King Kong 30 Candidate gene sequencing analysis: RM10927 and BGID25 markers correspond to the physical location of the reference genome of indica rice variety Minghui 63 at Chr01:14750992..14920510, with an interval of 169.5kb, including 34 annotated genes. Candidate genes near the co-segregated markers BGID36 and BGID34 in this region were preferentially selected for sequencing analysis.

[0038] (2) Use the candidate gene sequence obtained in step (1) to perform nucleotide sequence alignment through the sequence compariso...

Embodiment 2

[0080] Example 2: Hybridization of F using King Kong 30 / BG1222 2 Genetic and disease resistance phenotype verification of xa34-nv7InDel molecular marker in population

[0081] (1)F 2 Segregation population construction: F 1 Generation; F 1 F 2 generational segregation groups.

[0082] (2)F 2 About 100 strains were cultivated on a single plant in the paddy field, and the "leaf-cutting method" was used to inoculate Xanthobacterium oryzae V-type bacteria (strain number 5226) at the booting stage. The published literature is: "Chen et al., 2011. Genetic analysis and molecular mapping of a novel recessive gene xa34(t) for resistance against Xanthomonasoryzae pv. Oryzae. Theor Appl Genet, 122:1331-1338.") for identification of disease resistance phenotypes. Carry out disease resistance phenotype investigation 21 days after inoculation, investigate and evaluate resistance with reference to the method of International Rice Institute INGER (can refer to " INGERGenetic Resources C...

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Abstract

The invention discloses an InDel molecular marker realizing coseparation with the rice xanthomonas oryzae resistant gene xa34(t) as well as detection primers and applications of the InDel molecular marker. The InDel molecular marker has the nucleotide sequence as shown in SEQ ID NO.1. The InDel molecular marker is the co-dominant marker, can distinguish homozygotes from heterozygotes, and can be used for identifying the rice xanthomonas oryzae resistant variety. The InDel molecular marker is high in specificity, the band differences are great, and clear distinguishing can be realized through agarose gel electrophoresis. The invention further provides detection primers of the InDel molecular marker, which are composed of the primer as shown in SEQ ID NO.3 and the primer as shown in SEQ ID NO.4. The invention further provides a detection kit of the InDel molecular marker, which comprises the detection primers. With the detection primers or the detection kit, the molecular breeding process for the broad-spectrum xanthomonas oryzae resistance of rice is greatly accelerated.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and particularly relates to an InDel molecular marker co-separated with rice bacterial blight resistance gene xa34(t), detection primers and application thereof. Background technique [0002] Bacterial blight (Xanthomonas oryzae pv. oryzae) is one of the main diseases of rice in my country and Southeast Asia, which is the main rice producing area in the world, and seriously threatens the safety of rice production. Bacterial blight infection can harm the growth of rice, generally reducing yield by 10%-30%, and can exceed 50% in severe cases, or even no harvest [Mew, 1987, Current Status and Future Prospects of Research on Bacterial Blight of Rice.Ann Rev Phytopathol , 25:359-382.]. Since the disease is a vascular bacterial disease, the control effect of common chemical agents is not ideal, and the use of host resistance is an effective means to control the disease [Wu Shangzhong, 1982, R...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 汪聪颖陈深冯爱卿苏菁汪文娟封金奇陈炳伍圣远张梅英杨健源曾列先朱小源
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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