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Molecular marker tightly linked with Cucumis sativus tumor gene Tu

A technology of molecular markers and fruit tumor genes, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of backward genomics research of cucumbers and the inability to effectively obtain molecular markers, and accelerate the process. Effect

Inactive Publication Date: 2009-07-15
SHANGHAI JIAO TONG UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the backwardness of cucumber genomics research, there have been no examples of successfully using the map-based cloning method to clone genes in cucumbers. The main reason is that molecular markers closely linked to target genes cannot be effectively obtained.

Method used

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  • Molecular marker tightly linked with Cucumis sativus tumor gene Tu
  • Molecular marker tightly linked with Cucumis sativus tumor gene Tu
  • Molecular marker tightly linked with Cucumis sativus tumor gene Tu

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Step 1: Acquisition of Tu gene closely linked SRAP markers

[0029] 1. F 2 Group construction

[0030] The European greenhouse type inbred line S06 (female parent) has smooth surface without fruit tumors and smaller white thorns; the South China type inbred line S52 (male parent) has fruit tumors on the surface and thicker black thorns. The S06 inbred lines and S52 The inbred line has been disclosed in the article "Construction of Cucumber SRAP Genetic Linkage Map and Gene Mapping of Initiation Node" in "Progress of Natural Science" in 2005 (Pan Junsong et al. Construction of Cucumber SRAP Genetic Linkage Map and Gene Location of Initiation Node Orientation. Advances in Natural Sciences, 2005, 15, 167-172). This embodiment utilizes these two parents to prepare the hybrid combination, F 1 F 1 F 2 generation groups. In 247 strains F 2 In individuals, the presence / absence of fruit tumor phenotype was identified and validated by Chi-square analysis.

[0031] 2. Scre...

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Abstract

The invention discloses a molecular marker which is closely linked with tuberculate trait gene Tu in cucumis sativus, belonging to the technical field of genetic engineering. Two SRAP markers are firstly obtained, wherein, the marker ME6EM9 has the sequence in SEQ ID No.1 in a sequence table, and the marker ME2EM4 has the sequences in SEQ ID No.3 and SEQ ID No.4 in the sequence table. The ME6EM9 and ME2EM4 are transformed into stable SCAR markers which respectively are C_SC69 and C_SC24. The C_SC69 is dominant marker, and 218bp products in SEQ ID No.2 are amplified; C_SC24 is co-dominant marker which has two amplified products that are respectively 514bp in SEQ ID No.5 and 455bp in SEQ ID No.6. The molecular marker of the invention can be applied to assisted selection of tuberculate trait in cucumis sativus and can speed up the breeding process of cucumber varieties.

Description

technical field [0001] The invention relates to molecular markers in the technical field of genetic engineering, in particular to molecular markers closely linked with cucumber fruit tumor gene Tu. Background technique [0002] Cucumber (Cucumis sativus L.) is a year-round herbaceous plant of Cucurbitaceae (Cucurbitaceae). It is native to the warm and humid northern India at the southern foot of the Himalayas. It has been cultivated in my country for more than 2,000 years. my country is the second largest cucumber plant. center of origin. As one of the ten most important vegetable crops in the world, cucumber is also one of the main vegetable crops in my country. [0003] Fruit is the most important part of cucumber's economic traits. Cucumber fruit belongs to gourd, which is jointly developed by ovary and receptacle. An important character on cucumber fruit is fruit tumor, which is a tumor-like protrusion on the surface of cucumber fruit. The study of tuberculate genes be...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 张微微蔡润潘俊松何欢乐姚丹青
Owner SHANGHAI JIAO TONG UNIV
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