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InDel molecular marker for rapid screening of types of cucumber hard spine/soft spine fruits

A molecular marker, indel-ts1 technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problem of less research on cucumber epidermal hair genes

Pending Publication Date: 2017-07-11
XUZHOU NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

So far, relatively little research has been done on the genes of cucumber trichomes

Method used

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  • InDel molecular marker for rapid screening of types of cucumber hard spine/soft spine fruits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0016] Construction of a Genetic Segregation Population and Identification of Cucumber Softthorn Gene

[0017] 1. A variety of F 2 Group construction

[0018] Build F 2 The soft thorn variety used in the group is: NC073. Hard thorn varieties are: S77, SA419-2. In this example, these 3 parents were used to prepare a hybrid combination to obtain F 1 Generation, F 1 Generations are all hard thorn plants; F 1 F 2 Generation groups and BC 1 group. in F 2 and BC 1 Segregation of hard and soft spines appeared in the population, identification and statistics of hard and soft spines phenotypes, analysis of hard and soft spines in F 2 and BC 1 The segregation ratio in the experiment was verified by Chi-square analysis, and finally it was concluded that the soft thorn trait of cucumber was a recessive trait controlled by a single gene in the nucleus.

[0019] 2. Screening of Ts gene closely linked SSR markers and InDel markers

[0020] 2.1 Extraction of cucumber genomic DNA...

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Abstract

The invention provides an InDel molecular marker for rapid screening of types of cucumber hard spine / soft spine fruits. The InDel molecular marker is named as InDel-Ts1 and comprises 334 nucleotide sequences shown in the formula of SEQ ID NO. 1 in the sequence table. The InDel molecular marker is co-segregated from the soft-spine gene Ts. The InDel molecular marker has high stability and can realize simple, fast and high throughput screening of types of cucumber hard spine / soft spine fruits around the world.

Description

technical field [0001] The invention relates to molecular markers in the technical field of genetic engineering, in particular to an InDel molecular marker co-separated with cucumber fruit soft thorn gene Ts. Background technique [0002] Cucumber (Cucumissativus L.) is an annual herbaceous plant belonging to the Cucurbitaceae genus Cucumis. As one of the ten most important vegetable crops in the world, cucumber is also one of the main vegetable crops in my country. [0003] Fruit is the most important part of cucumber's economic traits. Cucumber fruit belongs to gourd, which is jointly developed by ovary and receptacle. An important character on cucumber fruit is fruit thorn, which is the specialized epidermis of cucumber. So far, there are relatively few studies on cucumber trichome genes. In 2015, Zhao J L et al. obtained 7936 strains of F 2 Population, using the BSA method to locate the Mict gene in the interval of 71.13kb genetic distance of cucumber No. 3 chromosome...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 杨绪勤蒋继宏袁博苏奕人孟来生薛羚伟王梦琪
Owner XUZHOU NORMAL UNIVERSITY
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