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Specific primer of molecular marker of low amylose content gene Wx-mq of rice and application of molecular marker specific primer

A technology of low amylose and molecular markers, applied in the field of crop molecular genetics and breeding, can solve the problems of high amylose content, increased gelatinization temperature, and reduced gel consistency, so as to speed up the breeding process, save costs, and identify Genetic Convenience Effects

Inactive Publication Date: 2018-06-01
天津市农作物研究所
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Problems solved by technology

Studies have shown that the amylose content in rice is high, the gelatinization temperature increases, and the gel consistency decreases, making the rice hard and poor in taste

Method used

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  • Specific primer of molecular marker of low amylose content gene Wx-mq of rice and application of molecular marker specific primer
  • Specific primer of molecular marker of low amylose content gene Wx-mq of rice and application of molecular marker specific primer
  • Specific primer of molecular marker of low amylose content gene Wx-mq of rice and application of molecular marker specific primer

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Experimental program
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Embodiment 1

[0024] (1) Materials and methods:

[0025] 1. Material: Contains homozygous recessive Wx-mq Genetic rice variety Nanjing 9108 (bred by the Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, available for sale), containing Wx-mq Allelic rice variety Longjing 31 (bred by Jiamusi Rice Research Institute of Heilongjiang Academy of Agricultural Sciences and Heilongjiang Longjing High-Tech Co., Ltd., available for sale), Nanjing 9108 / Longjing 31 / / Nanjing 9108 breeding materials (Breeding material of Tianjin Crop Research Institute).

[0026] 2. DNA extraction method: use CTAB method to extract the DNA of various varieties (lines) of rice. Take rice seedling leaves, add liquid nitrogen to grind into powder, and quickly transfer to 1.5 mL Eppendorf tube; add 800 μL of CTAB (1% w / v CTAB, 0.7 MNaCl, 10 mM EDT, 50 mM Tris-HCl, pH8. 0) Mix the extraction buffer, put it in a water bath at 65°C for 45 minutes, shake gently every 10 minutes, centrifuge at 12000 rpm for 10 ...

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Abstract

The invention discloses a specific primer of a molecular marker of a low amylose content gene Wx-mq of rice and application of the specific primer. An upstream primer WxF of the molecular marker is shown as SEQ ID NO.1, and a downstream primer WxR of the molecular marker is shown as SEQ ID NO.2; the size of an amplified product is 200bp. Through adoption of a detection method of the molecular marker of the low amylose content gene Wx-mq of the rice, which is provided by the invention, whether rice variety resources contain recessive and homozygous genes Wx-mq can be identified, and homozygousand heterozygous genotypes of the genes Wx-mq can be accurately distinguished, so that the selection efficiency of rice varieties containing the genes Wx-mq is greatly improved, and the rice eating quality breeding is quickened.

Description

technical field [0001] The invention belongs to the technical field of crop molecular genetics and breeding, and relates to rice low amylose content genes Wx- mq Molecular marker-specific primers and their applications. Background technique [0002] Rice is the largest food crop in my country and the staple food of our people. In recent years, my country's rice output has increased year by year. With the improvement of people's living standards, people's requirements for rice are not only reflected in the output, but also put forward higher requirements for the quality of rice, especially the quality of food. The eating quality of rice mainly includes aroma, palatability, rice stickiness, softness, etc.; cooking quality mainly includes amylose content, gel consistency, gelatinization temperature (alkali digestion value), etc. Studies have shown that the amylose content in rice is high, the gelatinization temperature increases, and the gel consistency decreases, making th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11C12Q1/686
CPCC12Q1/686C12Q1/6895C12Q2521/301C12Q2565/125
Inventor 王晓静孙林静孙玥张融雪闫双勇李军玲崔中秋苏京平王胜军
Owner 天津市农作物研究所
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