Molecular marker of wheat powdery mildew disease-resistant genes Pm51 and application of molecular marker
A technology of wheat powdery mildew and powdery mildew resistance gene, which is applied in the field of plant molecular genetics and wheat breeding, can solve the problems of resistance gene loss and resistance, and achieve the effect of accurate identification and accelerated utilization
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[0022] Decaploid E. elongatum is an excellent resistance source for wheat disease resistance breeding. In order to utilize the powdery mildew resistance gene in E. elongatum, we investigated the powdery mildew resistance gene in the introgression line CH7086 derived from E. elongatum. Pm51 is used for localization, development and application of functional molecular markers.
[0023] 1. Identification and analysis of powdery mildew at seedling stage
[0024] Four physiological races of powdery mildew were used to identify the powdery mildew resistance of each test material in vitro at the 2-4 leaf stage. Greenhouse sowing experiment materials After the first leaf is fully unfolded, cut the leaf segment about 2-4cm, place it horizontally on the surface of the culture medium, with the leaf facing up, pick the spores of powdery mildew fungus with a dissecting needle and inoculate it on the leaf segment. Place the inoculated leaf segments in a light incubator (20-23° C.), add lig...
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