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SSR (simple sequence repeat) marker for tomato yellow leaf curl disease resisting character co-segregation and application thereof

A technology for tomato yellow leaf curl and yellow leaf curl disease, which is applied to the SSR marker for co-separation of tomato yellow leaf curl disease resistance and its application field

Inactive Publication Date: 2016-10-26
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Claims
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Problems solved by technology

At present, there are few researches on ty-5 molecular marker assisted breeding in China

Method used

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  • SSR (simple sequence repeat) marker for tomato yellow leaf curl disease resisting character co-segregation and application thereof
  • SSR (simple sequence repeat) marker for tomato yellow leaf curl disease resisting character co-segregation and application thereof

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Embodiment Construction

[0013] The technical solution of the present invention will be further described below in conjunction with the accompanying drawings, but it is not limited thereto. Any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be covered by the present invention. within the scope of protection.

[0014] The invention provides a SSR marker for the co-separation of anti-yellow leaf curl disease traits of tomato and its application, the specific contents are as follows:

[0015] 1 Materials and methods

[0016] 1.1 Test material

[0017] The tested tomato disease-resistant parent CLN32120a-23 (provided by the Asian Vegetable Research and Development Center) and the susceptible parent Moneymaker (provided by the Institute of Vegetable and Flower Research, Chinese Academy of Agricultural Sciences) are both stable inbred lines of tomato varieties. T...

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Abstract

The invention discloses an SSR (simple sequence repeat) marker for tomato yellow leaf curl disease resisting character co-segregation and application thereof. The SSR marker includes primers: upstream primer T5-F: 5'-CAATCAATCGATGCACAAAACACC-3'; downstream primer T5-R: 5'-GACTGACTGCATTGGATTTGGCTT-3'. The marker can be amplified in CLN32120a-23 into a 580bp strip, can be amplified in Money marker into a 640bp strip and can be amplified in F1 into the above two strips, marking results are substantially consistent to that of field identification, it is proved that the marker can distinguish anti-disease materials, disease-sensitive materials and hybrid anti-disease materials and is a co-dominant marker in tight linkage with tomato yellow leaf curl disease virus resisting gene ty-5. Molecular marker detection is 90% matching with field detection in terms of detection results. The marker can be used in PCR (polymerase chain reaction) quick detection for tomato yellow leaf curl virus resisting ty-5 gene, and certain theoretical and practical basis is provided for the application of ty-5 molecular marker assisted selective breeding.

Description

technical field [0001] The invention relates to an SSR marker for co-separation of tomato yellow leaf curl resistance traits and a PCR method for assisted selection of TYLCV-resistant varieties by using the SSR marker. Background technique [0002] As a new type of viral disease, tomato yellow leaf curl virus has become one of the major diseases threatening tomato production in various countries due to its destructive characteristics and rapid disease spread. At present, cultivating disease-resistant varieties is still the most effective way to control yellow leaf curl virus disease. The resistance genes that have been excavated so far include Ty-1, Ty-2, Ty-3, Ty-3a, Ty-4, ty-5 and so on. The earliest identified anti-tomato yellow leaf curl virus (TYLCV) gene Ty-1 was derived from Chilean tomato LA1969, in which VERLAAN et al. showed that Ty-1 and Ty-3 are a pair of alleles, and their encoded RNA-dependent RNA polymerase (RDR) belongs to the RDRγ type, which has a typical...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68C12R1/94
CPCC12Q1/701C12Q2600/156
Inventor 杨欢欢许向阳李景富赵婷婷姜景彬刘冠
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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