New application of cocklebur fruit water extract in weeding aspect
A technique of extracting and cocklebur, applied in the field of pesticides
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[0010] Test sample: Xanthium medicinal materials were purchased from Nanjing Pharmaceutical Co., Ltd.
[0011] Weeds tested: rape (Brassica rapa L. Chinensis Group), bermudagrass (Cynodon dactylon L.), duckweed (Lemna minor L.) seeds, provided by Hubei Academy of Agricultural Sciences.
[0012] Experimental instruments: high-speed universal pulverizer; vertical automatic pressure steam sterilizer; shaker; ultrasonic oscillator; rotary evaporator; constant temperature water bath; electronic balance; intelligent artificial climate box; vacuum pump.
[0013] experimental method:
[0014] When rapeseed and bermudagras seeds were used as screening targets for herbicidal activity screening, the agar medium surface coating method was used. Use a 96-well cell culture plate, add 300μL of water agar to each well, add about 10 seeds to each well, add 20μL of the drug solution, and the concentration of the primary screening sample is 1.0mg / mL, 0.3mg / mL, 0.1mg / mL . One week later, check the seed...
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